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EC number: 211-995-8 | CAS number: 734-32-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- not applicable
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling schedule:
- Control : at 24 hours
- Test concentrations : at 0, 24, 48, and 72 hours in the freshly prepared media and at 24, 48,72 and 96 hours in the media after 24 hours of exposure - Vehicle:
- no
- Details on test solutions:
- PRETREATMENT OF THE TEST SUBSTANCE:
- 1000 mg of the test item were added to 10 litres of dilution water
- treated for 1 h in an ultrasonic bath and stirred for 24 h on a magnetic stirrer
- Undissolved particles were removed by filtration using a folded filter with a pore size of 7-12 µm - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- - Source : Interaquaristik.de Shop (Germany)
- Date supplied : 2016-01-11
- Acclimatisation : Stock held since 2016-01-11 and acclimatised to the test conditions since then
- Temperature : 20 - 24 °C
- Dissolved oxygen : > 5 mg/L
- Feeding : Commercial fish food, daily. Feeding discontinued 24 h prior to test start.
- Mortalities during acclimatisation period : < 5 %
- Medication : none
- Mean standard length (n = 42) : 2.82 cm (S.D. = 0.19 cm) - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 12.7 °dH (227 mg/L CaCO3)
- Test temperature:
- 21.5-21.7 °C (concerning the highest test concentration)
- pH:
- 7.9 (concerning the highest test concentration)
- Dissolved oxygen:
- 8.6-8.8 mg/L (concerning the highest test concentration)
- Salinity:
- n.a.
- Nominal and measured concentrations:
- Effective concentrations ranged from 87.3 % to 101.1 % of nominal values in the freshly prepared media and from 71.3 % to 95.2 % of nominal values in the media after 24 hours of exposure.
- Details on test conditions:
- EXPOSURE CONDITIONS:
- Test vessels: glass aquaria holding 5 L of test media covered by glass plates
- Experimental design: 5 test concentration plus 1 control
- Number of test species: 7 fish per test concentration
- Feeding during the exposure period: none
- Method of initiation: fish were placed in prepared media
- Loading: 0.33 g body weight (wet weight) per litre
- Photoperiod: 16 h light: 8 h dark
- Temperature: 21.1 to 21.7 °C
- Aeration: gentle aeration via narrow glass tubes
- Test item concentration/s: 1.56, 3.13, 6.25, 12.5 and 25 mg/L
- Method of administration: stock solution
- Medium renewal: daily
- Criteria of effects: The criterion of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 2, 24, 48, 72 and 96 hours, type and incidence of sub-lethal effects compared with control fish were observed - Reference substance (positive control):
- not required
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 17.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- All results are expressed in terms of nominal concentrations, because the relevant test item concentrations for the calculation of LCx-values were within the range of 80%-120% recovery during the test. Effective concentrations ranged from 87.3 % to 101.1 % of nominal values in the freshly prepared media and from 71.3 % to 95.2 % of nominal values in the media after 24 hours of exposure.
- Validity criteria fulfilled:
- yes
- Remarks:
- (mortality in the controls < 10% by the end of the test; dissolved oxygen concentration above 60% of the air-saturation value throughout the exposure period; pH did not vary by more than 1 unit)
- Conclusions:
- After 96 hours of exposure a LC50 of 17.634 mg/L was determined. All results are expressed in terms of nominal concentrations.
- Executive summary:
A study was performed to assess the acute toxicity to Danio rerio under static conditions. The study was conducted in accordance with Council Regulation (EC) No 440/2008, Method C.1 'Acute toxicity for Fish' (2008) which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992). A group of seven fish of the recommended size was exposed to 5 test concentration (1.56, 3.13, 6.25, 12.5 and 25 mg/L) dissolved in dilution water. Auxiliaries used to prepare the test media were an ultra turrax, a magnetic stirrer and a folded filter. Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. After 96 hours of exposure a LC50 of 17.634 mg/L was determined. All results are expressed in terms of nominal concentrations, because the relevant test item concentrations for the calculation of ECx-values were within the range of 80%-120% recovery during the test. Effective concentrations ranged from 87.3 % to 101.1 % of nominal values in the freshly prepared media and from 71.3 % to 95.2 % of nominal values in the media after 24 hours of exposure. This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute fish study.
Reference
Description of key information
The acute toxicity of the substance to fish (Danio rerio) was investigated in a semi-static test according to EEC methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 1 "Acute toxicity for Fish". After 96 hours of exposure a LC50 of 17.6 mg/L was determined. All results are expressed in terms of nominal concentrations.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 17.6 mg/L
Additional information
The relevant test item concentrations for the calculation of ECx-values were within the range of 80%-120% recovery during the test. Effective concentrations ranged from 87.3 % to 101.1 % of nominal values in the freshly prepared media and from 71.3 % to 95.2 % of nominal values in the media after 24 hours of exposure.
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