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Diss Factsheets
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EC number: 915-768-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4 July to 25 November 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ethyl 2-ethyl-6,6-dimethylcyclohex-2-ene-1-carboxylate
- EC Number:
- 261-020-5
- EC Name:
- Ethyl 2-ethyl-6,6-dimethylcyclohex-2-ene-1-carboxylate
- Cas Number:
- 57934-97-1
- Molecular formula:
- C13H22O2
- IUPAC Name:
- ethyl 2-ethyl-6,6-dimethylcyclohex-2-ene-1-carboxylate
- Reference substance name:
- Ethyl 2,3,6,6-tetramethylcyclohex-2-ene-1-carboxylate
- EC Number:
- 278-776-7
- EC Name:
- Ethyl 2,3,6,6-tetramethylcyclohex-2-ene-1-carboxylate
- Cas Number:
- 77851-07-1
- Molecular formula:
- C13H22O2
- IUPAC Name:
- ethyl 2,3,6,6-tetramethylcyclohex-2-ene-1-carboxylate
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Test material name (as stated in report): Givescone
Batch No. VE00409039
Appearance: Colourless to pale yellow liquid
Expiration date: January 21, 2018
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Sample preparation and Extraction: Liquid -liquid partitioning with toluene
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test system used in this study was the freshwater algae Pseudokirchneriella subcapitata (SAG 86.81).
The organisms were originally supplied by Sammlung von Algenkulturen, Albrecht-von- Haller-Institut, Universität Göttingen, Germany.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21-24 °C, controlled at ±2 °C
- pH:
- 8.1
- Nominal and measured concentrations:
- Nominal 1.00, 3.16, 10.0, 31.6 and 100% of a saturated solution at a loading rate of 100 mg/L, and a control
- Details on test conditions:
- Test organism:Pseudokirchneriella subcapitata
Test medium: OECD-Medium (modified)
Endpoints: ECx, (e.g. EC50, EC20, EC10), NOEC/LOEC
Biological parameters: Inhibition of growth in relation to control (growth rate & yield)
Test duration: 72 hours
Temperature (target): 21-24 °C, controlled at ±2 °C
Light regime: Permanent illumination
Light intensity (target):60-120 microEm-2s-1, within ±15% over the incubation area
Test units:60 mL glass vials, air-tightly closed by a screwcap with PTFE lining
Test concentrations (nominal): 1.00, 3.16, 10.0, 31.6 and 100% of a saturated solution at a loading rate of 100 mg/L, and a control
No. of replicates in the control: 6
No. of replicates per test concentration:3
Renewal of test solution during exposure: None (static system)
Chemical analysis of test concentrations: At start (0 hours) and at the end of exposure (72 hours)
Data evaluation:Shapiro-Wilk's Test on Normal Distribution; Levene's Test on Variance Homogeneity; Williams Multiple Sequential t-test for homogeneous variances; Student Test, Weibull analysis - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (K2Cr207)
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 15.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.43 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.51 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.33 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.84 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
Any other information on results incl. tables
Samples from the test solutions were analysed to determine actual levels of the test item at start of the exposure period (time 0) and following 72 hours of exposure. At time 0 hours the measured concentrations range between 80-100% of the expected concentrations following dilution of the water saturated solution. After 72 hours exposure, the test concentrations remained stable at between 88-102% of the time 0 hours starting concentration. Therefore, the test item concentration based on initial measured concentration remained stable within ±20% throughout the exposure period.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The ErC50 (72 hours) has been determined to be 15.4 mg/L and NOErC 1.43 mg/l under these test conditions.
- Executive summary:
An OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test) test was conducted for the substance Givescone in a static conditions. The ErC50 (72 hours) has been determined to be 15.4 mg/L and the NOErC, 1.43 mg/L under these test conditions.
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