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EC number: 428-310-5 | CAS number: 225789-38-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Name of test material (as cited in study report): Exolit OP 1230 - Molecular formula: C12H30O6P3Al - Molecular weight: 390.3 g/mol - Physical state: Solid, white powder - Analytical purity: 98.7 % - Purity test date: 2008-03-04 - Lot/batch No.: DEH2005977 - Expiration date of the lot/batch: 2012-02-22 - Storage condition of test material: Room temperature protected from moisture and light
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: The limit concentration and the control were analytically verified in the fresh media (0 h) and old media (48 h) by determination of the Phosphor (Phosphate) content with a cuvette test via UV-spectrophotometry.
- Sampling method: For the old media, separate replicates without test organisms were prepared and incubated under test conditions until analysis after 48 h.
- Sample storage conditions before analysis: All samples were stored at 6 ± 2 °C until start of analysis, if necessary. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 100 mg/L was freshly prepared with dilution water, mixed with an ultraturrax for 10 minutes at 24000 rpm and stirred with approximately 1100 rpm for 48 h at room temperature. Finally the dispersion was adjusted with 1 M NaOH to pH 7 ± 0.2 and filtered with a membrane filter.
- Eluate: Dilution water
- Differential loading: 100 mg/L Limit concentration
- Controls: Dilution water without test item tested under the same conditions as the test groups. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (STRAUS)
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- Lufthygiene (WaBoLu), further own breeding
- Age at study initiation (mean and range, SD): 2 to 24 h
- Method of breeding: In 2-3 L glass vessels with appr. 1.8 L culture medium, at 20 +/- 2 °C, in an incubator, 16 h illumination, illumination strength max. 20 µExm-2 x s-1
- Feeding during test: No feeding
ACCLIMATION
- Acclimation period: 2 h
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: No feeding
- Health during acclimation (any mortality observed): Healthy, no mortality observed
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No
- Hardness:
- Dilution water at test start: 164 (mg CaCO3/L)
- Test temperature:
- 20.3 °C
- pH:
- Water Quality Parameters at Test Start (0 h)
(measured in one additional replicate of the limit concentration and the control)
Nominal
Test Item
Concentration
[mg/L] pH-Value
100 7.12
Control 7.91
Water Quality Parameters at the Test End (48 hours)
(measured in all replicates)
Nominal
Test Item
Concentration
[mg/L] pH-Values
Replicates
1 2 3 4
100 7.58 6.96 6.95 6.96
Control 7.50 7.57 7.59 7.58 - Dissolved oxygen:
- 8.6 - 8.7 mg/l
- Salinity:
- Not measured, freshwater
- Details on test conditions:
- - Renewal rate of test solution (frequency/flow rate): One application at test start, static test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): None
OTHER TEST CONDITIONS
- Adjustment of pH: No
TEST CONCENTRATIONS
- Range finding study: Yes
Immobilization Rates of the Preliminary Range Finding Test
(n = 20, divided into 2 replicates with 10 daphnids each)
Nominal
Test Item
Concentration
[mg/L] IMMOBILIZATION [%]
24 h 48 h
Replicates Replicates
1 2 MV 1 2 MV
100 0 0 0 10 0 5
Control 0 0 0 0 0 0
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: 0 %
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Reported statistics and error estimates:
- The effects of the limit concentration were evaluated directly from the observation data without mathematical calculation.
- Validity criteria fulfilled:
- yes
- Conclusions:
- At the limit concentration 100 mg/L (nominal) of the test item no effect was observed.
- Executive summary:
In the acute immobilization test toDaphnia magna(STRAUS) the effects of the limitconcentration 100 mg/L of the test item were determined according to OECD 202 (2004). The limit test was conducted under staticconditions over a period of 48 h from 2010-08-02 to 2010-08-06, with the definitiveexposure phase of 48 h from 2010-08-04 to 2010-08-06 at DR. U. NOACK-LABORATORIEN,Käthe-Paulus-Str. 1,D-31157 Sarstedt, Germany .20 test organisms were exposed to the limitconcentration and the control. The limitconcentration and the control were analyticallyverified in the fresh media (0 h) and oldmedia (48 h) by determination of the Phosphor(Phosphate) content witha cuvette test via UV-spectrophotometry. Details ofthe analytical method are given in part 9. The recoveriesof the test item in the limitconcentration were determined tobe 107 % of the nominal valuein the fresh media at test start (0 h) and109 % in the old media at test end (48 h),demonstrating that concentrations of the test item were successfully maintained for theduration of the test. All effect values given are based on the nominal test item concentration.Water quality parameters (pH-value and dissolved oxygen concentration), measured in the fresh (0 h) an old media (48 h), were determined to be within theacceptable limits. The validity criteria of the test guideline were fulfilled.
EC50 nominal > 100 mg/L
NOEC = 100 mg/L
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- test substance stirred with the Ultra-Turrax for 24 h
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 2.1 - 2.5 Ca2+ - Mg2+/l
- Test temperature:
- 21.2 - 21.4°C
- pH:
- 7.3 - 7.6
- Dissolved oxygen:
- 8.4 - 8.6 mg/l
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Loss of concentration of the test substance over the duration of the test>20%. The test solution revealed clouding. Substance particles were determined on the surface of the water. No immobilization was determined up to the solubility limit.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this test the EC50 of the test item after 48 hours was > 100 mg/L (nominal concentration). No immobility occured up to the solubility limit of the test item in the test water.
- Executive summary:
The test substance was tested in Daphnia magna over 48 hours in a static system.
The concentration tested were 100 mg/L and a negative control (0 mg/L).
Before insertion of the test organisms the chamber contents were stirred for approximately 24 hours with a magnetic stirrer to ensure that solubility limit of the test substance in the test water is reached.
The aqueous deposits were observed on the water surface.
The substance concentration was determined in the 100 mg/L group. Because of the low water solubility of the test substance in water a concentration of only 66 mg/L was determined at study start. The concentration decreased to approx. 1 mg/L at study and. Dissolved and dispersed undissolved parts of the test substance contributed to the analytical result. The immobility values given in this report are based on nominal concentrations. The results based on the mean of the analytically determined concentrations are given in the brackets.
EC50 (48 hours) > 100 mg/L (33.7)
No immobility occurred up to the solubility limit of the test substance in the test water.
Referenceopen allclose all
Immobilization Rates of the Definitive Test after 24 and 48 h of Exposure
(n = 20, divided into 4 replicates with 5 daphnids each)
IMMOBILIZATION [%] |
||||||||||
Nominal |
24 h |
48 h |
||||||||
Replicates |
Replicates |
|||||||||
1. |
2. |
3. |
4. |
MV |
1. |
2. |
3. |
4. |
MV |
|
100 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Description of key information
On Key and one supporting study are avaialbel. In both studies a limit concentration 100 mg/L (nominal) of the test item no effect was observed. No immobility occured up to the solubility limit of the test item in the test water.
In both studies the EC50 48 -hours is > 100 mg/L. The NOEC = 100 mg/L
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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