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EC number: 217-978-1 | CAS number: 2028-63-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October 20, 2015 to December 17, 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study carried out according to internationally recognised guidelines and performed according to GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ECHA Guidance for the implementation of REACH, Guidance on information requirements and chemical safety assessment, Chapter R.7b: Endpoint specific guidance, November 2014 (version 2.0)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- But-3-yn-2-ol
- EC Number:
- 217-978-1
- EC Name:
- But-3-yn-2-ol
- Cas Number:
- 2028-63-9
- Molecular formula:
- C4H6O
- IUPAC Name:
- but-3-yn-2-ol
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): But-3-yne-2-ol
- CAS Number: 2028-63-9
- Physical state: colourless liquid
- Lot/batch No.: SC00013259
- Expiration date of the lot/batch: May 15, 2017
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of activated sludge: Domestic sewage works, Bois-de-Bay, Satigny, Switzerland.
- Sludge was collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium).
- The sludge was kept under aerobic conditions and used the same day. - Duration of test (contact time):
- 42 d
Initial test substance concentration
- Initial conc.:
- 30 mg/L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- APPARATUS
- Respirometer: Oxitop Control System
WATER
- Deionised water containing less than 10 mg/L dissolved organic carbon was used during the study.
STOCK SOLUTIONS
- Solution A: KH2PO4 8.5 g; K2HPO4 21.75 g, Na2HPO4.2H2O 33.4 g; NH4Cl 0.5 g (dissolved in water and made up to 1 L).
- Solution B: CaCl2 27.5 g (dissolved in water and made up to 1 L).
- Solution C: MgSO4.7H2O 22.5 g (dissolved in water and made up to 1 L).
- Solution D: FeCl3.6H2O 0.25 g; concentrated HCl one drop (dissolved in water and made up to 1 L).
MINERAL MEDIUM
- Solution A (50 mL) and deionised water (2000 mL) were mixed and Solution B (5 mL), Solution C (5 mL) and Solution D (5 mL) added before making up to 5 L with deionised water.
DETERMINATION OF DRY WEIGHT OF SUSPENDED SOLIDS
- Two 50 mL samples of the homogenised sludge were taken and water was evaporated on a steam bath, dried in an oven at 105-110 °C for two hours, and weighing the residue.
FLASK PREPARATION
- Test substance samples (13.10 mg, corresponding to 30 mg/L in 437 mL) were weighed in small aluminium boats and added to the contents of the test flasks of the Oxitop.
- For reference substance samples 43.70 mg (corresponding to 100 mg/L in 437 mL of test medium) were weighed in small aluminium boats and added to the contents of the test flasks of the Oxitop.
- Flasks were filled with 432 mL of mineral medium.
- Suspended sludge diluted to a concentration of 2.62 g/L dry matter was added.
- pH of each flask was not measured but assumed to be the same as the mineral medium.
- Two sodium hydroxide pellets were placed in the quivers on top of the bottle.
- The flasks were then closed tightly with the measuring heads and allowed to equilibrate to the test temperature.
- After temperature equilibration, the controller of the instrument starts the data acquisition (time zero of the experiment).
TEST CONDITIONS
- Temperature (21.3°C - 21.8 °C) and stirring were checked daily.
- Oxygen consumption of each flask was recorded daily.
NOMINAL CONCENTRATIONS
- Test material: 30 mg/L
- Reference substance: 100 mg/L
TOXICITY CONTROL
- An optional toxicity control was not performed. The validity of the study was not adversely affected.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Test performance:
- - The activity of the inoculum was verified.
- The repeatability validity criterion (not more than 20% difference between replicates) was fulfilled. Therefore, the test was considered valid.
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 35
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 49
- Sampling time:
- 42 d
BOD5 / COD results
- Results with reference substance:
- Biodegradation of reference substance sodium benzoate exceeded 40 % (after 7 days) and 65 % (after 14 days). For details see Appendix 4 of attached study report 15 - E052.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- 3-Butyne-2-ol undergoes 35% biodegradation after 28 days (49% after 42 days) in the test conditions. 3-Butyne-2-ol did significantly inhibit the intrinsic respiration of the iniculum at the test concentration at the beginning of the test. Toxicity towards the inoculum at test concentration can therefore not be excluded.
3-Butyne-2-ol should be regarded as not readily biodegradable according to this test. A repeat test at lower test substance concentration is advisable to complete the biodegradability assessment. - Executive summary:
The Ready Biodegradability of 3-Butyne-2-ol was determined by the Manometric Respirometry Test.
3-Butyne-2-ol undergoes 35% biodegradation after 28 days (49% after 42 days) in the test conditions. 3-Butyne-2-ol did significantly inhibit the intrinsic respiration of the iniculum at the test concentration at the beginning of the test. Toxicity towards the inoculum at test concentration can therefore not be excluded.3-Butyne-2-ol should be regarded as not readily biodegradable according to this test. A repeat test at lower test substance concentration is advisable to complete the biodegradability assessment.
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