[2-[[4-[(2-chloro-4-nitrophenyl)azo]phenyl]ethylamino]ethyl]trimethylammonium chloride

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

May 29, 1998

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

abstract

Remarks:

No information on the composition is available

Principles of method if other than guideline:

Only short abstract available. No information about guideline followed.

GLP compliance:

not specified

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Water media type:

freshwater

Total exposure duration:

96 h

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 35 mg/L

Conclusions:

LC50 = 35 mg/l

Executive summary:

Method

The test on was conducted with an unspecified method. Only short abstract is available for this study.

Conclusion

LC50 = 35 mg/l

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

From November 23 to 27, 1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Remarks:

The test was conducted on a similar substance. Further details were reported in the attachment at section 13.

Qualifier:

according to guideline

Guideline:

other: DIN 38 412 "TESTVERFAHREN MIT WASSERORGANISMEN (GRUPPE L). ALLGEMEINE HINWEISE ZUR PLANUNG, DURCHFUEHRUNG UND AUSWERTUNG BIOLOGISCHER TESTVERFAHREN (Ll)"

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

other: "BESTIMMUNG DER WIRKUNG VON WASSERINHALTSSTOFFEN AUF FISCHE - FISCHTEST (L15)", JUNE 1982.

Deviations:

not specified

GLP compliance:

no

Details on sampling:

- Concentrations: 10.0, 21.5, 46.4 and 100 mg/l.

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Preparation of the test substance: The product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria.
- Reasons for the selection of the concentrations: based on the results of 2 range finding studies and 1 main study not evaluated (LC50 after 96 h: between 32 and 100 mg/l).

Test organisms (species):

other: Leuciscus idus l., golden variety

Details on test organisms:

TEST ORGANISM
- Common name: Golden Orfe
- Strain: Leuciscus Idus L., Golden Variety
- Source: Fischzucht Paul Eggers D-2354 Hohenwestedt, Frg
- Length at study initiation: 5.3 Cm (Range: 4.7 - 5.7)
- Weight at study initiation: 3.7 G (Range: 2.1 - 4.9)
- Food type: Growing Feed F/B 50 Ssniff Spezialdiaeten Gmbh D-4770 Soest, Frg ad Libitum

ACCLIMATION
- Acclimation period: about 8 Weeks

OTHER CONDITIONS
- Water Temperature: 20 Centigrade +-1
- Medical Treatment: Twice With 0.05 Mg/L Malachite Green Chloride
- Housing: The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air.
- Mortality during the last 2 weeks of housing: about 0.2%
- Mortality during the adaptation period: 0%
- Medical Treatment: twice with 0.05 mg/l malachite green chloride once with 10 mg/l tetracycline hydrochloride

Details on test conditions:

TEST SYSTEM
- Test vessel: All-glass aquarium (30 cm x 22 cm x 24 cm)
- Aeration: continuous aeration with oil-free air
- No. of organisms per test concentrations: 10
- Loading (g fish / l test water): 3.7
- Adaptation to test water and test temperature: 3 days
- Withdrawel of food before exposure: 1 day

TEST MEDIUM / WATER PARAMETERS
- Test Water: reconstituted freshwater according to din 38 412, part 11, draft sept. 198].preparation from fully demineralized tap water conductivity: max. 10 micro mho
- Total Hardness : about 2.5 mmol/l
- Acid Capacity : about 5.5 mmol/l
- Ratio Ca/Mg Ions : 4 :1
- Ratio Na/K Ions : 10: 1
- Oxygen Content : > 60% of maximum saturation
- Test Medium:
294.0 mg/l CaCl2 . 2 H2O
123.3 mg/l MgSO4 .7 H2O
64.8 mg/l NaHCO3
5.8 mg/l KCl

OTHER TEST CONDITIONS
- pH : about 8.0
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

yes

Remarks:

Chloroacetamide

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 46.4 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

LC50 (48 h)= about 26 mg/l

Validity criteria fulfilled:

not specified

Conclusions:

46.4 mg/l

Executive summary:

Method

The short-term toxicity to fish was determined according to DIN 38 412 "Testverfahren mit wasserorganismen (gruppe l). allgemeine hinweise zur planung, durchfuehrung und auswertung biologischer testverfahren (ll)". The test was conducted at four different concentration: 10.0, 21.5, 46.4 and 100 mg/l.

Conclusion

46.4 mg/l <LC50< 100 mg/l

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

March 25, 1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

no guideline available

Principles of method if other than guideline:

Internal Method 11

GLP compliance:

no

Test organisms (species):

other: Trout

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

45 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

not specified

Validity criteria fulfilled:

not specified

Conclusions:

LC50 = 45 mg/l

Executive summary:

Only short abstarct is available. The LC50 value calculated in this test was 45 mg/l.

Description of key information

LC50 = 35 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

35 mg/L

Additional information

The substance was tested in order to evaluate the short-term toxicity to fish. No information about the method used for the execution of the study is available. The LC50 value calculated was 35 mg/l.

To support this information a test on an analogue molecule, with a different salification of the same core structure (Similar Substance 01) is available. Further information are reported in the Read Across justification attached to section 13.

The test on the Similar Substance 01 was determined according to DIN 38 412 "Testverfahren mit wasserorganismen (gruppe l). allgemeine hinweise zur planung, durchfuehrung und auswertung biologischer testverfahren (ll)". The test was conducted at four different concentrations: 10.0, 21.5, 46.4 and 100 mg/l. The LC50 value calculated for this test was in a range of 46.4 mg/l and 100 mg/l.

This result is in line with a further test on the Similar Substance 01, where the LC50 value calculated was 45 mg/l.