Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 241-012-8 | CAS number: 16941-92-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- not stated
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Not to current international guidleines, with significant deviations from the protocol. For example no revertant data provided, no metabolic activation, and no controls used.
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The spot test, first described by Iyer and Sybalsky (1958) and later by Ames et al. (1975).
- GLP compliance:
- not specified
- Type of assay:
- other: Spot test
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Species / strain / cell type:
- E. coli, other: B/r WP2 try- and WP2 hcr- try-
- Metabolic activation:
- without
- Test concentrations with justification for top dose:
- 0.001-10 M
- Details on test system and experimental conditions:
- Bacterial strains were spread on plates containing broth-enriched agar. Filter-paper disks containing the test substance were placed onto the plates. Revertant colonies around the disk were counted. Reversion assays with the test substance in liquid were also carried out with the two E.coli strains.
- Species / strain:
- other: all strains
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
- Conclusions:
- Interpretation of results (migrated information):
negative without metabolic activation
In a limited assay (Spot test), hexachloroiridic acid failed to induce reverse mutations in bacteria. - Executive summary:
In a limited assay (the spot test), filter paper discs containing hexachloroiridic acid were place onto agar plates containing bacterial strains Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538 or Escherichia coli strains B/r WP2 try- and WP2 hcr- try-. Revertant colonies around the discs were counted. Reversion assays with the test substance in liquid were also carried out with the two E.coli strains.
The test substance (at up to 10 M) failed to induce reverse mutations in bacteria.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
The genotoxicity data available on hexachloroiridic acid are limited.
In a non-guideline study - a rec assay for differential killing (as an indicator of DNA damage) using Basillus subtilis - gave a result which the investigators described as indicative of a mild positive rec effect (Kanematsu et al., 1980). [Likely the same study reported in Kada et al. (1980) and Leifer et al. (1981).]
In a limited bacterial mutation assay (the spot test), filter paper discs containing hexachloroiridic acid were place onto agar plates containing Salmonella typhimurium TA98, TA100, TA1535, TA1537 and TA1538 or Escherichia coli strains B/r WP2 try- and WP2 hcr- try-. Revertant colonies around the discs were counted. Reversion assays with the test substance in liquid were also carried out with the two E.coli strains. The test substance (at up to 10 M) failed to induce reverse mutations in bacteria (Kanematsu et al., 1980).
References not included elsewhere in IUCLID
Kada T, Hirano K and Shirasu Y (1980). Screening of environmental chemical mutagens by the rec-assay system with Bacillus subtilis. Chemical Mutagens - Principles and Methods for their Detection 6, 149-173.
Leifer Z, Kada T, Mandel M, Zeiger E, Stafford R and Rosenkranz HS (1981). Evaluation of tests using DNA repair-deficient bacteria for predicting genotoxicity and carcinogenicity: report of the U.S. EPA's Gene-Tox program. Mutation Research 211-297.
Justification for selection of genetic toxicity endpoint
No single, good-quality guideline study available. Limited in vitro data are available ("spot test" with Salmonella typhimurium and Escherichia coli; and rec assay with Bacillus subtilis).
Justification for classification or non-classification
Data are insufficient on which to confidently base classification. Classification, according to EU CLP critiera (1272/2008), for germ cell mutagenicity generally requires positive results from appropriate in vivo studies. As such, no classification is considered neccesary.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.