Benzenemethanaminium, N,N,N-trimethyl-, 2-hydroxy-2-methylpropanoate (1:1)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

benzyltrimethylazanium 2-hydroxy-2-methylpropanoate

EC Number:

805-580-6

Cas Number:

1431696-36-4

Molecular formula:

C14 H23 O3 N

IUPAC Name:

benzyltrimethylazanium 2-hydroxy-2-methylpropanoate

Details on test material:

- Identification: BTMA-HIB
- Chemical name: N,N,N-trimethyl -, 2-hydroxy- 2-methylpropanoate (1:1)
- Physical form: solid
- Water solubility: Miscible
- Purity: >90%; treated as 100% pure
- Storage conditions: at room temperature, under N2

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Observations of immobile Daphnia were made after 24 and 48 h of exposure.

Test solutions

Vehicle:

no

Details on test solutions:

Prior to the definitive test, a non-GLP range finding test with nominal concentrations
of BTMA-HIB of 1, 10 and 100 mg/l was performed.
Based on the results of this range finding test, the following nominal test
concentrations of BTMA-HIB for the definitive test were selected: 5, 10, 20, 40, 80
and 160 mg/l.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS
- Age: less than 24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.52 mmol/l

Test temperature:

22°C

pH:

7.1-7.4

Dissolved oxygen:

6.0-6.5 mg/L

Details on test conditions:

- Light: 16 h photoperiod a day, supplied by oberhead white fluorescent tubes
- Medium: Contious aerated Elendt M4 medium; prepared with deionised water (conductivity <1.5 µS/cm)
- Feed: Suspension of Desmodesmus subspicatus in Elendt M4 medium with an optical density OD680 of about 15 units; 400 μl per Daphnia per day, except on weekends
- Control of sensitivity: Acute reference test with potassium dichromate conducted twice a year
- Number of Daphnia: 40 individuals per test concentration, 20 per vessel
- Test vessel: 100 ml flasks, all-glass, with 50 ml of test medium
- Replicates: 2 replicates with test animals (20 animals per replicate); 3 replicates as blank controls

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

93.6 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 72.4-132

Applicant's summary and conclusion

Validity criteria fulfilled:

yes