Registration Dossier
Registration Dossier
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EC number: 258-964-5 | CAS number: 54079-53-7
Endpoint summary
Administrative data
Description of key information
Short term toxicity to fish:
A study was performed to assess the acute toxicity of test material to Danio rerio under static conditions. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity in Directive 92/69/EEC (O.J. No. L383A, 1992) Part C, Method 1 'Acute toxicity for Fish' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992).
A group of seven fish of the recommended size was exposed to a limit test concentration of nominally 1 mg/L of Macrolex Yellow 6G dissolved in water. Auxiliaries used to prepare the test media were an ultrasonic bath, a magnetic stirrer and a folded filter. The water solubility of test material is very low i.e
< 0.0007 mg/L. Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. The median lethal effect concentration LC50 was observed to be >1 mg/l . No toxic effect at against fishes at the limit of water solubility under exposure conditions. The water solubility of test material is < 0.0007 mg/L , hence indicating that aquatic toxicity is unlikely to occure.
Short term toxicity to aquatic invertebrate:
The test material is highly insoluble in water , the solubility was observed to be < 0.0007 mg/L , hence indicating that aquatic toxicity is unliky to occure.
Toxicity to aquatic algae and cyanobacteria:
The test material is highly insoluble in water , the solubility was observed to be < 0.0007 mg/L , hence indicating that aquatic toxicity is unliky to occure.
Toxicity to microorganism:
Effect of test material on activated sludge was evaluated according to Official Journal of the EC L 133 Part C: Biodegradability: examination of the respiratory Inhibition (largely corresponds to the test method OECD 209).A defined amount of activated sludge is made with synthetic Medium offset and measured the respiratory rate. These Respiration rate is compared with those used in test with different concentrations of the test substance be measured. The sensitivity of the activated sludge used is checked with 3,5-dichlorophenol as reference substance. Activated sludge (mixed population different microorganisms)were obtained from Laboratory plant mainly with municipal sewage. The EC50 valueof test material was observed to be >10000 mg/l after 30 min of respiration inhibition test.
The test material is highly insoluble in water , the solubility was observed to be < 0.0007 mg/L , hence indicating that aquatic toxicity is unliky to occure.
Additional information
Short term toxicity to fish:
A study was performed to assess the acute toxicity of test material to Danio rerio under static conditions. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity in Directive 92/69/EEC (O.J. No. L383A, 1992) Part C, Method 1 'Acute toxicity for Fish' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992).
A group of seven fish of the recommended size was exposed to a limit test concentration of nominally 1 mg/L of Macrolex Yellow 6G dissolved in water. Auxiliaries used to prepare the test media were an ultrasonic bath, a magnetic stirrer and a folded filter. The water solubility of test material is very low i.e
< 0.0007 mg/L. Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. The median lethal effect concentration LC50 was observed to be >1 mg/l . No toxic effect at against fishes at the limit of water solubility under exposure conditions. The water solubility of test material is < 0.0007 mg/L , hence indicating that aquatic toxicity is unlikely to occure.
Short term toxicity to aquatic invertebrate:
The test material is highly insoluble in water , the solubility was observed to be < 0.0007 mg/L , hence indicating that aquatic toxicity is unliky to occure.
Toxicity to aquatic algae and cyanobacteria:
The test material is highly insoluble in water , the solubility was observed to be < 0.0007 mg/L , hence indicating that aquatic toxicity is unliky to occure.
Toxicity to microorganism:
Effect of test material on activated sludge was evaluated according to Official Journal of the EC L 133 Part C: Biodegradability: examination of the respiratory Inhibition (largely corresponds to the test method OECD 209).A defined amount of activated sludge is made with synthetic Medium offset and measured the respiratory rate. These Respiration rate is compared with those used in test with different concentrations of the test substance be measured. The sensitivity of the activated sludge used is checked with 3,5-dichlorophenol as reference substance. Activated sludge (mixed population different microorganisms)were obtained from Laboratory plant mainly with municipal sewage. The EC50 valueof test material was observed to be >10000 mg/l after 30 min of respiration inhibition test.
The test material is highly insoluble in water , the solubility was observed to be < 0.0007 mg/L , hence indicating that aquatic toxicity is unliky to occure.
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