1,3,4,5-tetrahydroxycyclohexanecarboxylic acid

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study is not done according to OECD guideline. But it is a well documented study.

Data source

Reference

Reference Type:

publication

Title:

Untersuchungen über Thromboseprophylaxe mit organischen Salzen seltener Erden.

Author:

Vincke, E. and Never, H. E.

Year:

1944

Bibliographic source:

Zeitschrift fuer die Gesamte Experimentelle Medizin. 113:536

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

White mice (15 - 20 g each) were used as test animals. The route of administration was subcutaneous. The solution of the tested substance (concentration between 1 and 10 %) was applied under the dorsal skin of the mice and the duration of observation accounted for up to 6 days after injection.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,3,4,5-tetrahydroxycyclohexanecarboxylic acid

EC Number:

201-072-8

EC Name:

1,3,4,5-tetrahydroxycyclohexanecarboxylic acid

Cas Number:

77-95-2

Molecular formula:

C7H12O6

IUPAC Name:

(1S, 3R, 4S, 5R)- 1,3,4,5-Tetrahydroxy-cyclohexanecarboxylic acid

Test material form:

solid: crystalline

Details on test material:

- Name of test material (as cited in study report): quinic acid
- Other: The solution of quinic acid was neutralized with sodium hydroxide before application.

Test animals

Species:

mouse

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

White mice were used as test animals.
- Weight at study initiation: average weight between 15 g and 20 g

Administration / exposure

Route of administration:

subcutaneous

Vehicle:

not specified

Details on exposure:

The solution of the tested substance was applied under the dorsal skin of the mouse.

Doses:

Solutions with concentrations between 1 and 10% of the tested substance were applied.

No. of animals per sex per dose:

no data

Control animals:

not specified

Details on study design:

White mice (15 - 20 g each) were used as test animals. The route of administration was subcutaneous. The solution of the tested substance (concentration between 1 and 10 %) was applied under the dorsal skin of the mice and the duration of observation accounted for up to 6 days after injection. Clinical signs were briefly documented.

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LD50

Effect level:

10 other: g/kg bw

Based on:

test mat.

Mortality:

Mortality occurred under strong presyncope and exhaustion.

Clinical signs:

Strong presyncope and exhaustion leading to death were observed. The mice showed no signs of seizures.

Applicant's summary and conclusion

Conclusions:

The tested sodium salt of the quinic acid was tested for subcutaneous toxicity to mice. The subcutaneous toxicity is 10 g/kg bw.

Executive summary:

Neutralized quinic acid was tested for subcutaneous toxicity in mice. The sodium salt of the quinic acid showed a subcutaneous toxicity of 10 g/kg bw. From this result it can be concluded, that the subcutaneous toxicity of quinic acid is relatively low.