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EC number: 940-742-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- from October 22, 2007 to November 14, 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was carried out in accordance with internationally valid GLP principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Humic acids, potassium salts
- EC Number:
- 271-030-1
- EC Name:
- Humic acids, potassium salts
- Cas Number:
- 68514-28-3
- IUPAC Name:
- 68514-28-3
- Details on test material:
- - Name of test material (as cited in study report): Humic acids, potassium salts
- Molecular formula: not known - UVCB substance
- Molecular weight: not known - UVCB substance
- Substance type: technical product
- Physical state: solid
- Lot/batch No.: 16. 5. 2007/R
- Expiration date of the lot/batch: 05/2022
- Stability under test conditions: stable
- Storage condition of test material: dry conditions
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not required
Test solutions
- Details on test solutions:
- The test substance is soluble in water in concentrations needed for the test.
The stock solution was prepared by dissolution of 2.5 g of test substance in 500 mL deionised water on the day of testing.
In the preliminary test the concentrations were in the range of 46 - 1000 mg.L-1 with a geometric progression factor of 2.2.
In the main test the concentrations were in the range of 100 - 1000 mg.L-1 with a geometric progression factor of 1.8.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The activated sludge was collected two days before the day of testing.
After the sample collection the sludge was washed with potable water for 0.5 hour and subsequently decanted for 0.5 hour. This procedure was repeated three times in total.
Further the sludge was modified by addition of 50 mL of cultivation medium per 2 L of diluted sludge suspension at permanent aeration till the day of test.
The dry weight was determined from 10 mL of sludge suspension after 0.5-hour sedimentation.
Before the test the sludge was suspended in water up to concentration about 4000 mg of sludge dry weight per litre. The pH adjustment to 6.0 was carried out. In that way modified sludge suspension was aerated until the use.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 +/- 2°C
- pH:
- The pH value during the main test was in the range of 7.1 to 7.9.
- Nominal and measured concentrations:
- The preliminary test was performed using 5 concentrations in range 46 - 1000 mg.L-1 with a geometric factor of 2.2 to determine the concentrations to be used in the main test.
In the main test, 5 concentrations of the test substance in geometric progression with factor of 1.8 (100, 180, 320, 560, 1000 mg.L-1) were used together with two control experiments (without the test substance), one at the beginning and one at the end of the test. - Details on test conditions:
- Temperature: 20 +/- 2°C
Lighting: daily
Stirring and aeration: with filtered pressured air, airflow rate 0.8 L/min.
The pH value of reaction mixtures during the main test: approx. 7 – 8.
The deionised water was used for the preparation of solutions. Analytical grade reagents were used.
The stock solution of cultivation medium (synthetic nutrient medium):
Component Mass
peptone 16 g
meat extract 11 g
urea (NH2)2CO 3 g
sodium chloride NaCl 0.7 g
calcium chloride dihydrate 0.4 g
magnesium sulphate heptahydrate MgSO4.7H2O 0.2 g
anhydrous dipotassium hydrogen phosphate K2HPO4 2.8 g
water up to volume of 1000 mL
The pH value of this solution was 7.0-8.0.
The cultivation medium for sludge adaptation was always prepared fresh on the day of the use. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Under the current experimental conditions, all tested concentrations in the main test provided no significant inhibition of respiration rate. Therefore it was not possible to calculate the EC50 value.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
The 3h-EC50 value of reference substance, 3,5-dichlorophenol: EC50 = 11.4 mg.L-1 (95 % confidence interval: 10.6 – 12.3 mg.L-1) - Reported statistics and error estimates:
- Calculation of EC values
The values of EC50, EC20 and EC80 for the reference substance were calculated from equation of regression line prepared by commercial software Microsoft® Excel 2000.
The confidence intervals of EC50, EC20 and EC80 for the reference substance were calculated by the software QC.Expert 2.5 © 1998-2000 (product of TriloByte Ltd., Czech Republic).
Any other information on results incl. tables
Validity of test
The test met the following validity criteria:
- the respiration rates of two control experiments should not differ from each other by more than 15 %
- the EC50 value of the reference substance should be in the range of 5 – 30 mg.L-1
The difference of two respiration rates of control experiments at the beginning and the end of the main test from each other was:
1.74 mg.L-1.h-1 = 6.58 % (if the value of lower control 26.46 mg.L-1.h-1 = 100 %)
The 3h-EC50 value of reference substance, 3,5-dichlorophenol: EC50 = 11.4 mg.L-1 (95 % confidence interval: 10.6 – 12.3 mg.L-1)
Since all criteria for acceptability of the test were met, this study is considered to be valid.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
The influence of the test substance on the respiration rate of activated sludge was investigated after a contact time of 3 hours.
Test was performed according to:
Method C.11 - Activated Sludge Respiration Inhibition Test, Directive 88/302/EEC. Published in OJ L 133 1988.
The preliminary test was performed using 5 concentrations in range 46 - 1000 mg.L-1 with a geometric factor of 2.2 to determine the concentrations to be used in the main test.
In the main test, 5 concentrations of the test substance in geometric progression with factor of 1.8 (100, 180, 320, 560, 1000 mg.L-1) were used together with two control experiments (without the test substance), one at the beginning and one at the end of the test.
3,5-Dichlorophenol was used as reference substance. Five concentrations in geometric progression with a factor of 1.4 (5.2, 7.2, 10, 14 and 19 mg.L-1) were selected.
The abiotic decomposition was not detected even at the highest concentration of the test substance.
The pH value during the main test was in the range of 7.1 to 7.9.
On the basis of the effective concentrations describing the respiration inhibition of activated sludge caused by the test substance the EC50was found to be:
EC50> 1000 mg.L-1
Under the current experimental conditions, all tested concentrations in the main test provided no significant inhibition of respiration rate Therefore it was not possible to calculate the EC50 value.
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