Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 224-218-2 | CAS number: 4252-78-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented study according to international accepted guidelines and GLP compliant.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Deviations:
- yes
- Remarks:
- Two steps of treatment procedure was inverted. (Human error.) Presumed Effect on the Study: NONE
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- 2,2',4'-trichloroacetophenone
- EC Number:
- 224-218-2
- EC Name:
- 2,2',4'-trichloroacetophenone
- Cas Number:
- 4252-78-2
- Molecular formula:
- C8H5Cl3O
- IUPAC Name:
- 2-chloro-1-(2,4-dichlorophenyl)ethan-1-one
- Test material form:
- solid: crystalline
- Details on test material:
- Test item: DICHLOROPHENACYL CHLORIDE (CAS 4252-78-2)
Batch No. T-5427
Colour: beige
Physical state: solid, crystalline powder
Active Ingredient Content (GC): 98.9 %
Storage: on cool and dry place, protected from light
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- Species and strain: Crl:(WI)Br rats
Source: TOXI COOP Zrt.
Hygienic level at arrival: SPF
Hygienic level during the study: Good conventional
Number of animals: 3 animals/group
Sex: Female, nulliparous and non pregnant animals
Age of animals: Young adult rat, 8 weeks old in first, second and third step
Body weight range at starting (first step): 164 - 168 g
Body weight range at starting (second step): 155 - 173 g
Body weight range at starting (third step): 158 - 165 g
Animal health: Only healthy animals were used for the study. Health status was certified by the study director.
Housing: Group caging (3 animals/cage)
Light: Artificial light, from 6 a.m. to 6 p.m.
Temperature: 22 ± 3 °C
Relative humidity: 30 - 70 %
Ventilation: 10-15 air exchanges/hour by central air-condition system.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- acetone
- Remarks:
- Helianthi annui oleum raffinatum
- Details on oral exposure:
- A single oral administration - followed by a fourteen-day observation period - was performed by gavage.
Starting dose of 300 mg/kg bw was selected on the basis of the available information about the test item. The acute toxic class method was carried out involving a stepwise procedure with the use of 300 mg/kg bw as the starting dose in three female rats. No animal died in the first step at 300 mg/kg bw dose level the test was continued at 2000 mg/kg bw dose level on further three female rats. Since two animals died in the second step by Day 1, three further female rats were treated with the first dose (300 mg/kg bw). No animal died in the third step, so the test was finished. (Deviation: The step 2 and step 3 was inverted as follows: the animals of group 2 were treated with 2000 mg/kg bw dose and the animals of group 3 was treated with 300 mg/kg bw dose, but it doesn't have any presumed effect on the study.)
All doses were formulated in the vehicle. Concentration of formulations was adjusted to maintain a treatment volume of 10 mL/kg bw. The test item was applied in a concentration of 30 and 200 mg/mL. Formulations were prepared just before the administration and stirred continuously during the treatment.
The day before treatment the animals were fasted. The food but not water was withheld overnight. The food was given back 3 hours after the treatment. - Doses:
- 300, 2000 mg/kg bw
- No. of animals per sex per dose:
- 3 female/dose
- Control animals:
- no
- Details on study design:
- The observation period was 14 days. Animals were observed individually after dosing at least once during the first 30 minutes, then 1 h, 2 h, 3 h, 4 h, 5 h and 6 h after the treatment and twice each day for 14 days thereafter.
The body weights were recorded on day 0 (just before the treatment), on day 7 and on day 15 with a precision of 1 g.
At the end of the observation period rats were sacrificed and necropsy were performed.
Results and discussion
Effect levels
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 300 - 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No death occurred at 300 mg/kg bw single oral dose of the test item. All female rats in step 1 survived until the end of the 14-day observation period.
All rats dosed at 2000 mg/kg bw DICHLOROPHENACYL CHLORIDE (CAS 4252-78-2) died during the study. Two rats died on Day 1 and one animal died on Day 8. All deaths seemed to be consequences of systemic toxic effect of the test item. No death occurred at 300 mg/kg bw single oral dose of the test item. All female rats in step 3 survived until the end of the 14-day observation period. - Clinical signs:
- other: In group 1 treated with 300 mg/kg bw dose: Increased activity (score +1), irritability (score +1), blood around the nose (score +1) and piloerection (score +1; +2) occurred in all animals. Vocalization (score +1), incoordination (score +1) and diarrhea (s
- Gross pathology:
- All rats treated with 2000 mg/kg bw dose of the test item spontaneously died during the study. An external necropsy findings as undernourishing, piloerection, blood around the nose and anus contaminated with feces was detected in one animal. An internal necropsy finding as autolysis was observed in all animals. It is normal physiological process after death.
The animals treated with 300 mg/kg bw survived until the scheduled necropsy on Day 15. Internal necropsy finding as pale kidneys was observed in two animals of group 1 and in two animals of group 3. This alteration could not be related to the test item toxic effect, but was regarded an individual variation. Most likely the observation is a congenital anomaly. No pathological changes were found related to the effect of the test item during the macroscopic examination of survivor animals.
Any other information on results incl. tables
Groups |
Treatment |
Lethality |
|
Test item |
Dose(mg/kgbw) |
Females |
|
1 |
“dichlorophenacyl chloride” |
2000 |
3/3 |
2 |
“dichlorophenacyl chloride” |
300 |
2/3 |
3 |
“dichlorophenacyl chloride” |
300 |
0/3 |
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Remarks:
- Migrated information
- Conclusions:
- The method used, was not intended for the precise calculation of a precise LD50 value.
The test item was ranked into classes of Globally Harmonized Classification System (GHS) described in the OECD Guideline No. 423.
Hazard Category: Acute Tox. 4 - Executive summary:
Dose (mg/kg bw) Mortality (dead/treated) LD50 (mg/kg bw) GHS category 300 0/6 between 300 and 2000 4
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.