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EC number: 203-450-8 | CAS number: 106-99-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- hematoxicity
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-guideline experimental study with clearly reported methods and results, no indication as to GLP status. Adequate for assessment.
Data source
Reference
- Reference Type:
- publication
- Title:
- Comparative toxicity of known and putative metabolites of 1,3-butadiene in human CD34+ bone marrow cells.
- Author:
- Irons RD, Pyatt DW, Stillman WS, Som DB, Claffey DJ, Ruth JA.
- Year:
- 2 000
- Bibliographic source:
- Toxicology, 150:99–106.
Materials and methods
- Principles of method if other than guideline:
- This study evaluated the effect of several BD metabolites on colony formation and cytotoxicity in human CD34+ haematopoietic progenitor cells. The metabolites tested were: 3,4-epoxybutene (EB); two enantiomers of diepoxybutane (BDE), d,l-butane-bis-oxide and meso-butane-bis-oxide; and (2S,3R)-3-epoxybutane-1,2-diol (BDO).
- GLP compliance:
- not specified
- Type of method:
- in vitro
Test material
- Reference substance name:
- 3,4-epoxybutene
- IUPAC Name:
- 3,4-epoxybutene
- Reference substance name:
- D,L-butane-bis-oxide
- IUPAC Name:
- D,L-butane-bis-oxide
- Reference substance name:
- meso-butane-bis-oxide
- IUPAC Name:
- meso-butane-bis-oxide
- Reference substance name:
- (2S,3R)-3-epoxybutane-1,2-diol
- IUPAC Name:
- (2S,3R)-3-epoxybutane-1,2-diol
- Details on test material:
- the test materials are metabolites of 1,3-butadiene found both in vivo and in vitro
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Test animals
- Species:
- human
Results and discussion
Applicant's summary and conclusion
- Executive summary:
This study evaluated the effect of several BD metabolites on colony formation and cytotoxicity in human CD34+ haematopoietic progenitor cells. The metabolites tested were: 3,4-epoxybutene (EB); two enantiomers of diepoxybutane (BDE), d,l-butane-bis-oxide and meso-butane-bis-oxide; and (2S,3R)-3-epoxybutane-1,2-diol (BDO). Procedures involving volunteers were reviewed and approved by the institutional internal review board, and volunteers were recruited by informed consent.
Human CD34+ cells (>95% purity) were isolated from bone marrow obtained from volunteers and exposed to test substances (10-8–10-4M) and cytokines (granulocyte-macrophage/colony-stimulating factor, GM-CSF; erythropoietin, EPO; interleukin-3, IL-3; stem cell factor, SCF) for 16h at 37°C. Colonies were scored after 14 days as multi-lineage myeloid, i.e. granulocyte erythroid-megakaryocyte-macrophage (GEMM), granulocyte-macrophage (CFU-GM) or erythroid burst forming units (BFU-E).
The two BDE enantiomers caused a concentration-dependent suppression of clonogenic response, but EB and BDO were without effect. It is concluded that, in contrast to murine haematopoietic progenitor cells, human cells are insensitive to EB, and are only sensitive to BDE.
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