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EC number: 310-154-3 | CAS number: 121158-58-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1992-02-27 until 1992-03-27
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: partly not well documented: Inoculum concentration not mentioned; composition of test medium not specified
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Phenol, dodecyl-, branched
- EC Number:
- 310-154-3
- EC Name:
- Phenol, dodecyl-, branched
- Cas Number:
- 121158-58-5
- Molecular formula:
- C18H30O
- IUPAC Name:
- 4-(3,4,5,6-tetramethyloctan-2-yl)phenol
- Reference substance name:
- Dodecylphenol, branched
- IUPAC Name:
- Dodecylphenol, branched
- Details on test material:
- - Name of test material (as cited in study report): Dodecylphenol T
- Substance type: technical product
- Physical state: liquid
- Analytical purity: 100%
- Impurities (identity and concentrations): not mentioned
- Composition of test material, percentage of components: not mentioned
- Purity test date: not mentioned
- Lot/batch No.: continuously production; 3630/81325
- Date of production: 1991-04-25
- Stability under test conditions: 1 year
- Storage condition of test material: not mentioned
Constituent 1
Constituent 2
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: domestic sewage plant (Marl-East, Germany)
- Laboratory culture: no
- Storage conditions: not mentioned
- Storage length: not mentioned
- Preparation of inoculum for exposure: according to prescipt (not specified)
- Pretreatment: not mentioned
- Concentration of sludge: suspended solids and dry weight of inoculum not mentioned
- Initial cell/biomass concentration: 33*10E04 cfu/ml (colony forming units/ml)
- Water filtered: not mentioned - Duration of test (contact time):
- 29 d
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: not specified
- Additional substrate: none
- Solubilising agent (type and concentration if used): not used
- Test temperature: 21.0 - 22.8 °C
- pH: not mentioned
- pH adjusted: no
- Aeration of dilution water: not specified
- Suspended solids concentration: not mentioned
- Continuous darkness: not mentioned
- Other: none
TEST SYSTEM
- Culturing apparatus: 5000 ml glass vessels containing culture samples
- Number of culture flasks/concentration: 2 bottles, one concentration
- Method used to create aerobic conditions: not mentioned
- Method used to create anaerobic conditions: not applicable
- Measuring equipment: carbon analyzer (Shimadzu)
- Details of trap for CO2: collection of carbon dioxide as sodium carbonate with sodium hydroxide solution (no further details mentioned)
- Other: none
SAMPLING
- Sampling frequency: on days 0, 3, 7, 12, 17, 26, 28 and 29
- Sampling method: not mentioned
- Sample storage before analysis: not mentioned
- Other: none
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes (1 vessel with inoculum without test substance)
- Abiotic sterile control: not performed
- Toxicity control: not performed
- Other: none
STATISTICAL METHODS: no statistics performed
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 20 mg/l final test medium (11.66 mg DOC/l)
Results and discussion
- Preliminary study:
- not performed
- Test performance:
- Procedure: The test substance was poured into a defined, liquid mineral medium, which was inoculated with an inoculum from activated sludge and aerated at 21.0 - 22.8 °C. The released CO2 is bound in caustic soda solution in the form of sodium carbonate. The degradation was followed over the duration of the test of 29 days at the days of sampling as stated above via a TIC analysis (total inorganic carbon) of the bound CO2. One day before the end of the test (28th day) the remaining dissolved CO2 was driven out by acidification of the test preparation, the degradation values ascertained on the 29th day (end of incubation) are therefore related to the 28th day.
Preparation of the mineral medium: Two days before the beginning of the test (addition of the test substance) 35 l of the mineral medium were prepared and aerated overnight. On the following day 2400 ml were filled into the individual test containers and, after inoculation with the inolculum, filled with the mineral medium up to 3 l.
Dosage of the test material: The test material was weighed exactly by means of a weighing aid (reaction vessel from Eppendorf, cut-off cover) and was put into the test vessels together with the weighing aid.
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 25
- Sampling time:
- 28 d
- Remarks on result:
- other: test substance (10 mg/l)
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 6
- Sampling time:
- 28 d
- Remarks on result:
- other: test substance (20 mg/l)
- Details on results:
- see below
BOD5 / COD results
- Results with reference substance:
- see below
Any other information on results incl. tables
Table: Degradation kinetic
sampling time [days] | degradation [%] | |||
test substance 10 mg/l | test substance 20 mg/l | sodium benzoat 20 mg/l | ||
0 | 0 | 0 | 0 | |
3 | 4 | 1 | 58 | |
7 | 6 | 1 | 83 | |
12 | 10 | 3 | 90 | |
17 | 15 | 4 | 95 | |
21 | 19 | 5 | 96 | |
26 | 23 | 6 | 96 | |
28 (after acifidation) | 25 | 6 | 95 |
Applicant's summary and conclusion
- Interpretation of results:
- under test conditions no biodegradation observed
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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