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EC number: 218-216-0 | CAS number: 2082-79-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline with acceptable restrictions (neither TA102 or WP2uvrA strains were used to detect cross-linking mutagens, positive control with S9-mix tested only with TA100, no result of positive control was reported)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- neither TA102 or WP2uvrA strains were used to detect cross-linking mutagens, positive control with S9-mix tested only with TA100, no result of positive control was reported
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate
- EC Number:
- 218-216-0
- EC Name:
- Octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate
- Cas Number:
- 2082-79-3
- Molecular formula:
- C35H62O3
- IUPAC Name:
- octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate
Constituent 1
Method
- Target gene:
- Salmonella typhimurium: histidine operon
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- with S9 mix (S9 fraction of liver from rats induced with Aroclor 1254 plus co-factors)
- Test concentrations with justification for top dose:
- without S9 mix: 10, 25, 50, 100 and 250 µg/0.1 ml;
with S9 mix: 5, 10, 25, 50 and 100 µg/0.1 ml. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- cyclophosphamide
- Remarks:
- with S9 mix
Migrated to IUCLID6: 100 and 250 µg/0.1 ml physiological saline for TA100
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
NUMBER OF REPLICATIONS: 3
OTHER: Positive control experiments in the form of Spot Tests were carried out simultaneously with the following substances: N-methyl-N'-nitro- N-nitrosoguanidine (2µg/0.1 ml for TA1535), 9-aminoacridine (100 µg/0.1 ml for TA1537), daunoblastin (50 µl/0.1 ml for TA98), 4-nitroquinoline-n-oxide (10 µl/0.1 ml for TA100). - Evaluation criteria:
- When the colonies had been counted, the arithmetic mean was calculated. The test substance was considered to be non-mutagenic if the colony count in relation to the negative control was not doubled at any concentration.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Mean number of colonies of histidine-prototrophic back-mutants (without S9 mix)
Dose group |
TA98 |
TA100 |
TA1535 |
TA1537 |
Control |
21 |
116 |
13 |
5 |
10 µg/0.1 ml |
27 |
96 |
18 |
5 |
25 µg/0.1 ml |
25 |
103 |
20 |
7 |
50 µg/0.1 ml |
28 |
95 |
14 |
6 |
100 µg/0.1 ml* |
22 |
107 |
15 |
3 |
250 µg/0.1 ml* |
24 |
94 |
16 |
7 |
Mean number of colonies of histidine-prototrophic back-mutants (with S9 mix)
Dose group |
TA98 |
TA100 |
TA1535 |
TA1537 |
Control |
34/29 |
208/190 |
22/19 |
10/9 |
5 µg/0.1 ml |
30/36 |
187/169 |
19/19 |
6/7 |
10 µg/0.1 ml |
26/30 |
194/172 |
16/22 |
8/7 |
25 µg/0.1 ml |
35/30 |
197/196 |
20/22 |
8/10 |
50 µg/0.1 ml* |
39/31 |
193/197 |
17/16 |
8/10 |
100 µg/0.1 ml* |
32/36 |
162/188 |
16/20 |
8/9 |
n/n: first figures are values without, second figures values with pre-incubation.
* Precipitation
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
No evidence of the induction of point mutations by the test compound or by the metabolites of the substance formed as a result of microsomal activation was detectable in the strains of S. typhimurium used in these experiments.
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