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EC number: 204-622-5 | CAS number: 123-35-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 09 December 2013 - 12 December 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Well conducted and well described study in accordance with GLP and OECD Guideline No 202.
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not applicable - Analytical monitoring:
- yes
- Details on sampling:
- Concentrations: single samples for analysis were taken from the control and all test concentrations from replicates without daphnids (except at the end of the test : replicates with daphnids).
Sampling method: samples were taken at the start (t = 0 h) and every 24 h thereafter in old and new solutions and the control.
Storage: samples were analysed directly after sampling. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
Method: slow-stirring in a closed vessel with minimum headspace
The test sample formed a clear colourless solution and appeared to be completely soluble and stable when mixed with test water at the concentrations prepared.
Controls: test water without test substance but treated in the same way as the test substance solutions. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
Common name: Daphnia magna (Straus)
Strain: Clone 5
Source: LIEBE - CNRS UMR 7146 - UFR SciFA - Université de Lorraine Campus Bridoux - Bât. IBISE, 8, rue du Général Delestraint - 57070 METZ, bred in the Laboratoires des Pyrénées et des Landes
Age at study initiation: less than 24 h
Method of breeding: parthenogenesis
Feeding during the test: no
ACCLIMATION
Acclimation period: 48 h
Acclimation conditions: same as test
Type and amount of food: mix of 3 algae strains: Chlorella vulgaris = 2.5x10^6 cells/mL/day/daphnid, Desmodesmus subspicatus = 2.5x10^6 cells/mL/day/daphnid and Pseudokirchneriella subcapitata = 5x10^6 cells/mL/day/daphnid
Feeding frequency: daily
Only daphnids not older than 24 hours were used for the test
VALIDITY OF BATCH OF DAPHNIDS
Health during acclimation (any mortality observed): daphnids originated from a healthy stock, showing no signs of stress such as mortality, presence of males, ephippia or discoloured animals. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- not applicable
- Hardness:
- 250 mg/L (CaCO3)
- Test temperature:
- 20°C +/- 2°C
- pH:
- 7.73-7.97 (whole test period)
- Dissolved oxygen:
- 8.21-8.82 mg/L (whole test period)
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 1.0, 1.3, 1.7, 2.3, 3.0 and 4.0 mg/L
Measured concentrations: 0.655, 0.928, 1.161, 1.552, 1.968 and 2.994 mg/L (averaged) - Details on test conditions:
- TEST SYSTEM
Test vessel: glass test tubes sealed with screwcaps
Volume: 20 mL (without headspace)
Aeration: no
Renewal rate of test solution: daily
No. of organisms per vessel: 5
No. of vessels per concentration (replicates): 4
No. of vessels per control (replicates): 4
3 abiotic replicates for control and test concentration
Biomass loading rate: 5/20 mL
TEST MEDIUM / WATER PARAMETERS
Source/preparation of dilution water: reconstituted water, as prescribed by OECD Guideline 202
Conductivity: < 10µS/cm
Intervals of water quality measurement: water temperature was recorded continuously throughout the test. Dissolved oxygen concentrations and pH were recorded at the start of the test (t = 0 h), at t = 24 h (new and old solutions) and at the end of the test (t = 48 h) in all vessels.
OTHER TEST CONDITIONS
Adjustment of pH: no
Photoperiod: 16h light and 8h dark
EFFECT PARAMETERS MEASURED
Immobility and abnormal behaviour were determined by visual observation after 24 and 48 h. The daphnids were considered to be immobile if they were not able to swim within 15 seconds after gentle agitation of the test vessels.
TEST CONCENTRATIONS
Spacing factor for test concentrations: 1.3
Range finding study
Test concentrations: 0.1, 1.0 and 2.0 mg/L
Results used to determine the conditions for the definitive study: 70% inhibition was observed at 2.0 mg/L and 100% inhibition was observed at the maximum solubility. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.47 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95%CL: 1.366-1.578
- Details on results:
- no details
- Results with reference substance (positive control):
- Results with reference substance are valid.
EC50: 0.98 mg/L - Reported statistics and error estimates:
- The software ToxRat® Professional was used for the determination of the effective concentrations. The EC50 value was determined by Probit analysis using linear max. likelihood regression.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48h-EC50 of MYRCENE on Daphnia magna was 1.47 mg/L, based on mean measured concentrations.
Based on the results of this study, MYRCENE is classified hazardous to the aquatic environment under Regulation (EC) No 1272/2008 (CLP) - Category Acute 1 and Category Chronic 2. - Executive summary:
An acute aquatic toxicity study was performed on Daphnia magna in accordance with GLP and OECD Guideline No 202. Following a preliminary range-finding test, twenty daphnids (four replicates, five daphnids per replicate) were exposed to an aqueous solution of MYRCENE at the required nominal test concentrations of 1.0, 1.3, 1.7, 2.3, 3.0 and 4.0 mg/L. Immobilisation of daphnids was determined in a semi-static 48-h test by visual observation after 24 and 48 h.
The concentrations of the test item were measured at the start (t = 0 h), at t = 24 h (new and old solutions) and at the end of the test (t = 48 h). Concentrations measured in freshly prepared solutions and in 24-h old solutions revealed that the concentrations of the test item were not satisfactorily maintained within ± 20 % of the initial concentrations. Thus, the evaluation of the effects on Daphnia magna was based on the geometric mean of the measured concentrations: 0.655, 0.928, 1.161, 1.552, 1.968 and 2.994 mg/L.
The lowest observed effect concentration based on immobilisation was 1.161 mg/L (measured concentration). At 1.968 mg/L (measured concentration), 100% of daphnids were immobilised. No sublethal effects were reported.
Therefore, the 48h-EC50 of MYRCENE for Daphnia magna was estimated to be 1.47 mg/L, based on mean measured concentrations.
Based on the results of this study, MYRCENE is classified hazardous to the aquatic environment under Regulation (EC) No 1272/2008 (CLP) - Category Acute 1 and Category Chronic 2.
Reference
Description of key information
An acute aquatic toxicity study was performed on Daphnia magna in accordance with GLP and OECD Guideline No 202.
Immobilisation of daphnids was determined in a semi-static 48-h test by visual observation after 24 and 48 h.
The evaluation of the effects on Daphnia magna was based on the geometric mean of the measured concentrations: 0.655, 0.928, 1.161, 1.552, 1.968 and 2.994 mg/L.
The lowest observed effect concentration based on immobilisation was 1.161 mg/L (measured concentration). At 1.968 mg/L (measured concentration), 100% of daphnids were immobilised. No sublethal effects were reported.
The 48hr-EC50 was 1.47 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1.47 mg/L
Additional information
Key sudy conducted under GLP according to OECD 202 Guideline and EU Method C2.
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