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Diss Factsheets
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EC number: 246-690-9 | CAS number: 25167-70-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant, guideline study, available as unpublished report, no restrictions, fully adequate for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1300 (Acute inhalation toxicity)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 2,4,4-trimethylpentene
- EC Number:
- 246-690-9
- EC Name:
- 2,4,4-trimethylpentene
- Cas Number:
- 25167-70-8
- Molecular formula:
- C8H16
- IUPAC Name:
- 2,4,4-trimethylpent-1-ene
- Details on test material:
- - Name of test material (as cited in study report): Diisobutylene
- Sample ID Number ID# GCRD10401R (to reference the other Lyondell DIB studies).
- Physical state: Clear light brown liquid
- Chemical composition of sample of diisobutylene: approximately 70% 2,4,4-trimethylpentene (detailed chemical composition information provided in Table 1).
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Crl:CD®(SD)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories, Raleigh, North Carolina, USA
- Weight at study initiation: males 261-276 g; females 202-213 g
- Age at study initiation: males Approximately 8 weeks
- Fasting period before study:
- Housing: Individual suspended wire-mesh cages except during inhalation when they were placed in a wire-mesh battery containing 10 separate cages
- Diet: PMI Nutrition International, LLC, Certified Rodent Lab Diet® 5002 ad libitum except during exposure
- Water: Municipal water ad libitum except during exposure
- Acclimation period: Minimum of 7 days
ENVIRONMENTAL CONDITIONS
- Temperature: 21.2-21.7°C
- Humidity: 41.7-53.7%
- Air changes (per hr): Not reported
- Photoperiod: 12 hrs dark / 12 hrs light
IN-LIFE DATES: From: 8 November 2005 To: 22 November 2005
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
Exposure apparatus: 130 L glass and stainless steel whole-body inhalation chamber operated at a minimum of 10 air changes per hour. Exposure atmosphere conditions were recorded approximately every 30 minutes during the exposure. Oxygen content was measured pre-exposure. The time required to attain 99% of the equilibrium concentration (or clearance time for the concentration to decrease from the equilibrium concentration) was calculated.
A vapour atmosphere of the test article was generated and piped to the inlet of the whole body chamber where it was mixed with the chamber supply air. Exhaust atmosphere was filtered (particulate) prior to entering the in-house exhaust system. Test article flow rate = 0.75 mL/min; supply airflow rate 26.9 L/min; chamber ventilation rate = 30.6 L/min.
TEST ATMOSPHERE
- Brief description of analytical method used: Two primary compounds of the test article, DIB-1 (2,4,4-trimethyl-1-pentene) and DIB-2 (2,4,4-trimethyl-2-pentene), were analyzed independently with each sample obtained by gas chromatography.
- Samples taken from breathing zone: yes
- Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 4185 ppm
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Mortality - at the approximate midpoint of exposure, immediately following exposure on study day 0 and twice daily thereafter for 14 days; Clinical obs: observed for mortality at the approximate midpoint of exposure, immediately following exposure on study day 0 and twice daily thereafter for 14 days; Bodyweights: Immediately prior to exposure on day 0 and on days 7 and 14.
- Necropsy of survivors performed: yes - Statistics:
- None (limit test, no mortalities)
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 19 171 mg/m³ air
- Remarks on result:
- other: >4185 ppm
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 4 185 ppm
- Remarks on result:
- other: No mortalities
- Mortality:
- No mortalities
- Clinical signs:
- other: Clinical observations immediately following exposure included red material around the nose, mouth and eye, white and clear material around the mouth and yellow material on the urogenital area. Yellow material on the urogenital area was noted during the 14
- Body weight:
- There was no effect on bodyweight
- Gross pathology:
- There were no gross findings for any animals at the scheduled necropsy
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The acute LC50 of diisobutylene was greater than 4185 ppm when male and female albino rats were exposed via whole-body to a vapour of the test
article for a single, 4-hour period. - Executive summary:
The acute inhalation toxicity of diisobutylene was evaluated in a 4-hour, single exposure study in male and female albino rats. There were no mortalities and no overt signs of toxicity. The acute LC50 of diisobutylene was greater than 4185 ppm, equivalent to 19171 mg/m3 air.
Diisobutylene does not require classification under DSD or CLP.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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