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EC number: 700-422-1 | CAS number: 61320-65-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: according to guideline and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD TG 431 and EC B40
- GLP compliance:
- yes
Test material
- Reference substance name:
- methyl 5-amino-4-cyano-3-methylthiophene-2-carboxylate
- EC Number:
- 700-422-1
- Cas Number:
- 61320-65-8
- Molecular formula:
- C8 H8 N2 O2 S
- IUPAC Name:
- methyl 5-amino-4-cyano-3-methylthiophene-2-carboxylate
- Details on test material:
- content 99.1 %
batch no. BOS 2589
Constituent 1
Test animals
- Species:
- other: in vitro
- Strain:
- other: in vitro
- Details on test animals or test system and environmental conditions:
- in vitro test
Test system
- Controls:
- other: in vitro test: negative control 0.9 % NaCl
- Amount / concentration applied:
- 50 µl
- Duration of treatment / exposure:
- 3 min and 60 min (3 inserts per period)
- Observation period:
- after the exposure period of 3 min or 60 minthe inserts were washed carefully in PBS and MTT reduction assay was performed
- Number of animals:
- in vitro test
- Details on study design:
- The experiment was carried out in accordance with tje OECD 431 guideline as well as with an EC guideline (amending Council Directtive 67/548/EEC B 46 skin corrosion) using commercially available reconstructed human epidermis(RHS) model EST-1000. Undiluted diazokomponente Rot B was applied topically to the RHS model: 25 mg of the test item per insert ( plus 30µl 0.9 % NaCl to moisten and ensure good contact wtih the skin; RT: three inserts per period of incubation).Aafter the exposure period of 3 min or 60 min the inserts were washed carefully in PBS. Afterwards the MTT reduction assay was performed to measure the cell viability.
Results and discussion
In vitro
Resultsopen allclose all
- Irritation / corrosion parameter:
- other: other: absorption: at 570 nm
- Value:
- 2.32
- Remarks on result:
- other:
- Remarks:
- Basis: other: negative control. Time point: 3 min. Max. score: 2.32. Reversibility: no data. Remarks: Optical Density (OD):2.32 = 100 % viability. (migrated information)
- Irritation / corrosion parameter:
- other: other: absorption at 570 nm
- Value:
- 2.26
- Remarks on result:
- other:
- Remarks:
- Basis: other: test item. Time point: 3 min. Max. score: 2.32. Reversibility: no data. Remarks: OD mean 2.32 = 100 % viability; OD mean 2.26 = 95.38 % viability. (migrated information)
- Irritation / corrosion parameter:
- other: other: absorption at 570 nm
- Value:
- 2.32
- Remarks on result:
- other:
- Remarks:
- Basis: other: negative control. Time point: 60 min . Max. score: 2.32. Reversibility: no data. Remarks: Optical Density (OD):2.32 = 100 % viability. (migrated information)
- Irritation / corrosion parameter:
- other: other: absorption at 570 nm
- Value:
- 2.16
- Remarks on result:
- other:
- Remarks:
- Basis: other: test item. Time point: 60 min. Max. score: 2.32. Reversibility: no data. Remarks: OD mean 2.32 = 100 % viability; OD mean 2.16 = 97.88 % viability. (migrated information)
Applicant's summary and conclusion
- Interpretation of results:
- other: not corrosive
- Executive summary:
An in-vitro study for predicting non-specific, corrosive potential of Diazokomponente Rot B by using reconstructed human skin (RHS) EST-1000 in accordance with OECD TG 431 and with an EC guideline (amending Concil Direcrive 67/548/EEC B 40 Skin corrosion). Diazokomponente Rot B was tested undissolved (25 mg per insert, plus 50 µl 0.9 % NaCl to moisten and to ensure good contact with the skin) and an incubation time of 3 min or of 60 min was used to determine time related toxic effects. After incubation the MTT reduction assay was performed to measure the cell viability which was 95 % and 98 % , respectively. Thus, the results show that no corrosive property of the test inem was determined by the assay used (Bayer AG 2011).
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