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EC number: 202-808-0 | CAS number: 99-99-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From: 18/03/1986 To: 21/03/1986
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
- Reference Type:
- secondary source
- Title:
- 4-Nitrotoluene CAS 99-99-0
- Author:
- OECD SIDS
- Year:
- 2 003
- Bibliographic source:
- SIDS Initial Assessment Report for 17th SIAM, UNEP Publications
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 99-99-01
- IUPAC Name:
- 99-99-01
- Reference substance name:
- 4-nitrotoluene
- EC Number:
- 202-808-0
- EC Name:
- 4-nitrotoluene
- Cas Number:
- 99-99-0
- Molecular formula:
- C7H7NO2
- IUPAC Name:
- 1-methyl-4-nitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): p-nitrotoluol
- Physical state: solid (yellow-green crystals)
- Analytical purity: 99%
- Storage condition of test material: in the dark at 22 °C
Constituent 1
Constituent 2
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Hoechst AG, Kastengrund, Germany. Conventional breeding
- Age at study initiation: 3-5 months
- Weight at study initiation: 2.7-3.7 kg
- Housing: the animals were housed in individual cages in air conditioned room
- Diet (e.g. ad libitum): Altromin 2123 Haltungsdiät-Kaninchen (Altromin GmbH, Lage/Lippe, Germany) and hay as well
- Water (e.g. ad libitum): deionised and chlorinated water from automatic drinking trough
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20+/-3 °C
- Humidity (%):50+/-20 %
- Photoperiod (hrs dark / hrs light): 12 hrs dark / 12 hrs light
IN-LIFE DATES: From: 18/03/1986 To: 21/03/1986
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: the untreated eye served as control
- Amount / concentration applied:
- Amount applied: 100 mg
- Duration of treatment / exposure:
- 24 hour(s)
- Observation period (in vivo):
- 24, 48, and 72 hours
- Number of animals or in vitro replicates:
- 3
- Details on study design:
- REMOVAL OF THE TEST SUBSTANCE
Washing (if done): yes, with physiologic saline solution
- Time after start of exposure: at 24 hours after exposure, and at all the other time points. The washing was performed also when excretions were observed in the treate eyes and when the fluorescein test was performed on the cornea of the treated eyes.
SCORING SYSTEM: Draize Method
TOOL USED TO ASSESS SCORE: fluorescein
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0.3
- Max. score:
- 3
- Reversibility:
- fully reversible within: 48 hours
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0.7
- Max. score:
- 3
- Reversibility:
- fully reversible within: 48 hours
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0.3
- Max. score:
- 3
- Reversibility:
- fully reversible within: 48 hours
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: not applicable
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: not applicable
- Irritation parameter:
- iris score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: not applicable
- Irritant / corrosive response data:
- One hour post exposure all the animals presented a light and whit discharge. The conjunctiva was swollen in all the animals and showed a clear hyperaemia of the vasculature.
24 hours post exposure the animals showed still a clear hyperaemia of the vasculature up to carmine red coloration of the conjunctiva.
The effect described above were completely reversible within 48 hours.
One hour post exposure all the animals presented a light and whit discharge. The conjunctiva was swollen in all the animals and showed a clear hyperaemia of the vasculature.
24 hours post exposure the animals showed still a clear hyperaemia of the vasculature up to carmine red coloration of the conjunctiva.
The effects described above were completely reversible within 48 hours.
Any other information on results incl. tables
Timeendpoint after application | 24h | 48h | 72h | Average (24-72 h)animal 1 | Average (24-72h) animal 2 | Average(24-72 h)animal 3 | Total average | ||||||
Animal number | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | - |
Conjuctiva: Chemosis | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Conjuctiva: redness | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0.3 | 0.7 | 0.3 | 0.4 |
Iris | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Corneal opacity | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Fluoresceintest | 0 | 0 | 0 |
|
|
| 0 | 0 | 0 |
Applicant's summary and conclusion
- Executive summary:
Hofmann T (1986)
In a test according to OECD TG 405, 100 mg of 4-nitrotoluene was applied into the conjunctival sac of the left eye of each of three rabbits. 24 hours later the eyes were rinsed. One hour post exposure all the animals presented a light and whit discharge. The conjunctiva was swollen in all the animals and showed a clear hyperaemia of the vasculature.
24 hours post exposure the animals showed still a clear hyperaemia of the vasculature up to carmine red coloration of the conjunctiva.
The effects described above were completely reversible within 48 hours.
4-Nitrotoluene is not irritating to the eyes of rabbits.
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