Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-824-2 | CAS number: 74-95-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study reasonably well reported and adequately validated therefore results can be considered reliable 2.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 994
Materials and methods
- Principles of method if other than guideline:
- The partition coefficient (PC) of a chemical in skin is an indicator of the capacity for the chemical in the skin and may reflect the rate at which a chemical penetrates the skin and enters into systematic circulation. This method measures the skin:air PC for volatile organic chemicals.
Clipped whole-thickness skin was obtained from dorsal surface of 8- to 16- week-old male F-344 rats. After removal of the hypodermis, skin was cut into strips and placed on the side of a glass vial. An organic chemical vapor was introduced into a sealed sample vial (initial concentration before equilibrium was 203ppm) and a corresponding reference vial, which were equilibrated at 32oC. Headspace concentrations at equilibrium were used to determine the skin:air PC value. - GLP compliance:
- no
Test material
- Reference substance name:
- Dibromomethane
- EC Number:
- 200-824-2
- EC Name:
- Dibromomethane
- Cas Number:
- 74-95-3
- Molecular formula:
- CH2Br2
- IUPAC Name:
- dibromomethane
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- rat
- Strain:
- Fischer 344
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- Rats were group-housed (3/ cage) in clear plastic cages with wood chip bedding. Water and feed Purina Formula (No. 5008) were available ad libitum. the ambient temp was maintained at 22+/- 2oC and light was regulated on a 12-hour light /dark cycle (starting at 0600 hour).
Administration / exposure
- Details on in vitro test system (if applicable):
- Pieces of skin, either miced to 1-mm cubes or cut to 1x0.5-cm strips, were the best sizes to compare. Dorsal skin of a rat was clipped using an elecric clipper immediately after euthanasia with carbon dioxide. Afetr cooling the skin, the hypodermis was removed, the skin was cut into pieces with a razor blade, and the pieces were placed on the walls of scintillation vials (24.65 ml volume) without saline.
Results and discussion
Applicant's summary and conclusion
- Conclusions:
- Dibromomethane quickly enters the skin as shown by the high Skin:air PC value 68.3.
- Executive summary:
The partition coefficient (PC) of a chemical in skin is an indicator of the capacity for the chemical in the skin and may reflect the rate at which a chemical penetrates the skin and enters into systematic circulation. In this simple method to measure the skin:air PC for volatile organic chemicals is presented.
Important considerations in the development of this method for a skin:air PC were the effect of size and shape of skin sample, initial chemical concentration, and time to equilibrium in the skin. Clipped whole-thickness skin was obtained from dorsal surface of 8- to 16- week-old male F-344 rats. After removal of the hypodermis, skin was cut into strips and placed on the side of a glass vial. An organic chemical vapor was introduced into a sealed sample vial (initial concentration before equilibrium was 203ppm) and a corresponding reference vial, which were equilibrated at 32oC. Headspace concentrations at equilibrium were used to determine the skin:air PC value.
The skin:air PC values correlated with previously determined vapor permeability constants but correlated poorly with octanol/water PC values.
After incubating samples for various times, the equilibration time for dibromomethane is skin was determined to be 4 hours. There was no statistical between minced skin and stripes of skin. Dibromomethane quickly enters the skin as shown by the high Skin:air PC value 68.3.
This method provides screening technique for predicting skin penetration of volatile chemicals. Further studies, in which the stratum corneum and viable epidermis are separated, are needed to account for partitioning into these discrete physiological compartments.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.