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Diss Factsheets
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EC number: 210-180-4 | CAS number: 609-15-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to OECD Guideline for Testing of Chemicals 431: In vitro Skin Corrosion: Human Skin Model Test (Original Guideline adopted April 13, 2004).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline for Testing of Chemicals 431: In vitro Skin Corrosion: Human Skin Model Test (Original Guideline adopted April 13, 2004).
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- EACA
- IUPAC Name:
- EACA
- Details on test material:
- Identity: EACA
Batch-No.: 001008
Purity: 93.9%
Stability in Solvent: Not relevant
Storage: At 2 8 °C
Retest Date: July 26, 2010
Constituent 1
Test system
- Amount / concentration applied:
- Each two tissues of the human skin Model EST1000 were treated with either the test item, the negative or the positive control for 3 minutes and 1 hour
50 µL of the test item and the controls were applied to each tissue
the test item was tessted neat
the negative control was deionised water
the positive control was 8.0 N potassium hydroxide - Duration of treatment / exposure:
- the tissues were tretaed for 3 minutes and 1 hour, respectively
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- % tissue viability
- Value:
- ca. 10.7
Any other information on results incl. tables
Table 1: Results after treatment with EACA
Dose Group |
Ex-posure Inter-val |
Absorbance 570 nm |
Absorbance 570 nm |
Mean Absorbance of 2 Tissues |
Rel. Absorbance [% of Negative Control]** |
Negative Control |
3 min |
2.036 |
2.066 |
2.051 |
100.0 |
Positive Control |
3 min |
0.118 |
0.092 |
0.105 |
5.1 |
EACA |
3 min |
0.506 |
0.411 |
0.458 |
22.3 |
Negative Control |
1 hour |
1.646 |
1.939 |
1.792 |
100.0 |
Positive Control |
1 hour |
0.030 |
0.025 |
0.028 |
1.5 |
EACA |
1 hour |
0.187 |
0.195 |
0.191 |
10.7 |
* Mean of three replicate wells after blank correction (0.037 for the 3 minutes treatment, 0.036
for
the 1 hour treatment)
** relative
absorbance [rounded values]:
The optical evaluation of the MTT-reducing capacity of the test item after a 1 hour incubation with MTT-reagent did not show evidence of a blue colour and thereby was not considered to be an MTT reducer.
The test item is considered to be corrosiveto skin, since
· the viability after 3 minutes exposure is less than 50%, and
· the viability after 1 hour exposure is less than 15%.
Applicant's summary and conclusion
- Interpretation of results:
- corrosive
- Remarks:
- Migrated information Criteria used for interpretation of results: other: OECD Guideline for Testing of Chemicals 431: In vitro Skin Corrosion: Human Skin Model Test (Original Guideline adopted April 13, 2004).
- Conclusions:
- In conclusion, it can be stated that in this study and under the reported experimental conditions, the test item EACA was corrosive to skin. According to EC Regulation 1272/2008 the test item is classified as corrosive (category 1).
- Executive summary:
This in vitro study was performed to assess the corrosive potential of EACA by means of the Human Skin Model Test. This test consists of topical application of the test material to the skin tissue for 3 minutes and 1 hour, followed by immediate determination of the cytotoxic effect. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity measured by formazan production from MTT at the end of the exposure period. Produced amount of formazan is measured photometrical at 570 nm.
Independent duplicate tissues of the human skin model EST-1000 were exposed to either the test item, the negative control or the positive control for 3 minutes and 1 hour, respectively.
The liquid test item (50 µL) was applied to each tissue and spread evenly over the surface of the tissue.
A volume of 50 µL of either the negative control (deionised water) or the positive control (8.0 N KOH) was applied to each tissue.
After exposure to the negative control the absorbance values exceeded the required acceptability criterion of mean OD570= 0.8 for both treatment intervals thereby confirming the acceptable quality of the tissues. Both absorbance values exceeded the minimum range of at least 20 times blank value(based on blank values of 0.037 for the 3 minutes treatment and 0.036 for the 60 minutes treatment the threshold for the 3 minutes treatment is 0.74 and 0.72 for the 60 minutes treatment).
Exposure to the positive control induced a decrease in the relative absorbance as compared to the negative control, both for the 3 minutes exposure period and for the 1 hour exposure period thus confirming the validity of the test system.
After exposure to the test item EACA the relative absorbance values decreased to 22.3% after 3 minutes. After the 1 hour exposure relative absorbance values were reduced to 10.7%. Both values exceeded the threshold for corrosivity of 50% for the 3 minutes exposure and 15% for the 1 hour exposure. Therefore, the test item was considered to be corrosive.
In conclusion, it can be stated that in this study and under the reported experimental conditions, the test item EACA was corrosive to skin. According to EC Regulation 1272/2008 the test item is classified as corrosive (category 1).
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