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EC number: 203-924-4 | CAS number: 111-96-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 7 Sept - 12 Sept 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well performed study equivalent to current OECD Guidelines with quality inspection; E. coli not tested
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- E. coli not tested
- GLP compliance:
- no
- Remarks:
- study performed before GLP
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Bis(2-methoxyethyl) ether
- EC Number:
- 203-924-4
- EC Name:
- Bis(2-methoxyethyl) ether
- Cas Number:
- 111-96-6
- Molecular formula:
- C6H14O3
- IUPAC Name:
- 1-methoxy-2-(2-methoxyethoxy)ethane
- Reference substance name:
- Diglyme
- IUPAC Name:
- Diglyme
- Reference substance name:
- Diethylenglycol-Dimethylether
- IUPAC Name:
- Diethylenglycol-Dimethylether
- Details on test material:
- Colorless liquid
Constituent 1
Constituent 2
Constituent 3
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella thyphimurium TA98, TA100, TA1535, TA1537, TA1538
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 0.005, 0.01, 0.1, 1.0, 5.0, 10.0, 25.0 and 50.0 µL/plate
- Vehicle / solvent:
- Distilled water
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Distilled water
- Positive controls:
- yes
- Positive control substance:
- other: without S9 mix: TA98/1538 - 2-Nitrofluorene; TA100/1535 - Sodium azide; TA1537 - 9-Aminocaridine; with S9 mix: 2-Anthramine
- Details on test system and experimental conditions:
- - Dosage Selection
All tests are run at a minimum of 4 concentration. In the standard plate test, at least 6 dose levels of the test material, dissolved in a suitable solvent, are added to the test system. The standard test doses are 0.005, 0.01, 0.1, 1.0, 5.0 and 10.0 µL/plate.
- Mutagenicity Testing
the procedure is based on the Ames publication (1975) and performed as follows:
(1) Nonactivation Assay
To a sterile test tube placed in a 43°C water bath the following is added in order:
(a) 2.00 mL of 0.6% agar containing 0.05 mM Histidine and 0.05 mM Biotin
(b) 0.05 mL of a solution of Diethylene glycol dimethyl ether to give approximate dose
(c) 0.1 mL - 0.2 mL of indicator organisms
(d) 0.5 mL of 0.01M Phosphate buffer, pH 7.4
This mixture is swirled gently and then poured into minimal agar plates . After the top agar has set, the plates are incubated at 37°C for approx. 2 days. The number of his+ revertant colonies growing on the plates is counted.
(2) Activation assay
The activation assay is run concurrently with the nonactivation assay. The only difference is the addition of 0.5 mL of S9 mix (S9 supernatant from Aroclor 1254 induced adult male Sprague-Dawley rats) to the tubes in place of 0.5 mL of Phosphate buffer which is added in nonactivation assays. All other details are similar to the procedure for nonactivation assays.
- Control compounds
A negative control consisting of the solvent (distilled water) used for the test material is performed in all cases. For negative controls, step (b) of Nonactivation assays is replaced by 0.05 mL of the solvent. The negative controls are employed for each indicator strain and are performed in the absence and presence of S9 mix.
Specific positive control compounds known to revert each strain are also used in the assays:
(a) Nonactivation
- Sodium azide (1 µg/plate): TA100, TA1535
- 2-Nitrofluorene (10 µg/plate): TA98, TA1538
- 9-Aminocridine (50 µg/plate): TA1537
(b) Activation
- 2-Anthramine (2.5 µg/plate): all strains
- Evaluation criteria:
- (1) Strains TA1535, TA1537 and TA1538
If the solvent control value is within the normal range, a test material that produces a positive dose response over three times concentrations with the highest increase equal to three times the solvent control values will be considered to be mutagenic.
(2) strains TA98 and TA 100
If the solvent control vallue is within the normal range, a test material that produces a positive dose response over three concnetrations with the highest increase equal to twice the solvent control value for TA98 and TA 100 will be considered to be mutagenic.
(3) Reproducibility
If a test material produces a response in a single test that cannot be reproduced in additional runs, the initial positive test data lose significance.
The preceding criteria are not absolute, and other extenuating factors may enter into a final evaluation decision. However, these criteria will be applied to the majority of situations. - Statistics:
- None
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Summary of mean number of revertants in Salmonella typhimurium with and without metabolic activation (negative and positive controls)
Concentration |
TA 98 |
TA 100 |
TA1535 |
TA 1537 |
||||||||
[µL/plate] |
- S9 mix |
+ S9 mix |
- S9 mix |
+ S9 mix |
- S9 mix |
+ S9 mix |
- S9 mix |
+ S9 mix |
||||
0 |
25 |
42 |
121 |
121 |
21 |
13 |
13 |
21 |
||||
0.05 |
24 |
36 |
126 |
104 |
10 |
8 |
19 |
16 |
||||
0.01 |
22 |
37 |
128 |
98 |
15 |
10 |
13 |
11 |
||||
0.1 |
34 |
36 |
109 |
109 |
15 |
10 |
15 |
16 |
||||
1.0 |
25 |
27 |
127 |
130 |
16 |
6 |
7 |
14 |
||||
5.0 10.0 25.0 50.0 |
27 30 35 36 |
35 32 40 45 |
130 137 173 173 |
110 146 109 146 |
15 18 - - |
14 5 - - |
9 10 - - |
9 14 - - |
||||
|
|
|
|
|
|
|
|
|
||||
Positive: |
|
|
|
|
|
|
|
|
||||
9-Aminoacridine |
|
|
|
|
|
|
518 |
|
||||
Sodium azide |
|
|
1433 |
|
1119 |
|
|
|
||||
2-Nitrofluorene |
1170 |
|
|
|
|
|
|
|
||||
2-Anthramine |
|
2109 |
|
2631 |
|
592 |
|
499 |
||||
|
||||||||||||
Concentration |
TA 1538 |
|
|
|
||||||||
[µL/plate] |
- S9 mix |
+ S9 mix |
|
|
|
|
|
|
||||
0 |
21 |
23 |
|
|
|
|
|
|
||||
0.05 |
14 |
29 |
|
|
|
|
|
|
||||
0.01 |
11 |
19 |
|
|
|
|
|
|
||||
0.1 |
19 |
22 |
|
|
|
|
|
|
||||
1.0 |
14 |
33 |
|
|
|
|
|
|
||||
5.0 10.0 25.0 50.0 |
19 20 - - |
36 22 - - |
|
|
|
|
|
|
||||
|
|
|
|
|
|
|
|
|
||||
Positive: |
|
|
|
|
|
|
|
|
||||
9-Aminoacridine |
|
|
|
|
|
|
|
|
||||
Sodium azide |
|
|
|
|
|
|
|
|
||||
2-Nitrofluorene |
1367 |
|
|
|
|
|
|
|
||||
2-Anthramine |
|
|
|
|
|
|
|
|
||||
|
|
1876 |
|
|
|
|
|
|
||||
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results:
negative
Diethylene glycol dimethyl ether was not genotoxic in any of the bacterial reverse mutation assays (Ames test) performed with Salmonella th. TA98, TA100, TA1535, TA1537 and TA1538. - Executive summary:
Diethylene glycol dimethyl ether was examined for mutagenic activity in Salmonella th. The compound was tested directly and in the presence of liver microsomal enzyme preparations from Aroclor-induced rats.The dose range employed for the evaluation of this compound was from 0.005 to 50 µL/plate.
Diethylene glycol dimethyl ether exhibited slight toxicity in the activation assay with the strain TA1535 at 10 µL/plate.
The results of the tests conducted on Diethylene glycol dimethyl ether in the presence and absence of metabolic activation were all negative.
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