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Diss Factsheets
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EC number: 481-150-8 | CAS number: 500011-86-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 3-15-2006 to 318-2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented, GLP and OECD 201 guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Remarks:
- The study was conducted according to the guideline in effect at time of study conduct.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Remarks:
- The study was conducted according to the guideline in effect at time of study conduct.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- DBC80
- IUPAC Name:
- DBC80
- Reference substance name:
- -
- EC Number:
- 481-150-8
- EC Name:
- -
- Cas Number:
- 500011-86-9
- Molecular formula:
- C9H5BrClN3O2
- IUPAC Name:
- 3-bromo-1-(3-chloropyridin-2-yl)-1H-pyrazole-5-carboxylic acid
- Reference substance name:
- 1H-Pyrazole-5-Carboxylic Acid, 3-Bromo-1-(3-Chloro-2-Pyridinyl)-
- IUPAC Name:
- 1H-Pyrazole-5-Carboxylic Acid, 3-Bromo-1-(3-Chloro-2-Pyridinyl)-
- Details on test material:
- - Name of test material (as cited in study report): IN-CBD80
- Molecular formula: C9H5BrCIN3O2
- Physical state: Tan powder
- Analytical purity: 96.75
- Lot/batch No.: 008
- Expiration date of the lot/batch: 7/22/08
- Stability under test conditions: Stable
- Storage condition of test material: Stored in the dark at room temperature.
- Other: Supplied by DuPont Company, Stine- Haskell Research Center Crop Protection Products, Newark, Deleware.
Constituent 1
Constituent 2
Constituent 3
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: High and low concentrations, positive and negative controls.
- Sampling method: Collected in glass containers at the beginning and end of the study.
- Sample storage conditions before analysis: Not specified, sent to Department of Analytical Chemistry for analysis.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: Negative and vehicle controls.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): <0.1 mL/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): No
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Source (laboratory, culture collection): In-house culture, original source Marinco Bioassy Laboratory.
- Age of inoculum (at test initiation): 3 days
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- Not applicable
- Test temperature:
- 20.9-23.6° Celcius
- pH:
- 8.01-9.54
- Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal- Control, Vehicle control, 3.5, 6.3, 11.4, 20.6, 37.1, 66.7, and 120 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): Closed, with non-absorbant cotton plugs.
- Material, size, headspace, fill volume: Glass 250 mL conical flasks, 100 mL fill volume
- Initial cells density: 71667 cells/mL
- Control end cells density: 1,876,667 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Distilled water
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: yes, to 8.1 with 0.1 N HCl or 0.1 N NaOH
- Photoperiod: Continuous
- Light intensity and quality: 7900-7985 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Growth rate and percent inhibition, at 0, 24, 48 and 72 hours.
- Determination of cell concentrations: Visual count using an Improved Neubaur Haemocytometer and microscope.
TEST CONCENTRATIONS
- Range finding study concentrations: 15, 30, 60 and 120 mg/L
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- other: EyC50
- Effect conc.:
- 54.49 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks on result:
- other: 95% CL 54.063-54.917 mg/L
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 3.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Reported statistics and error estimates:
- EyC50 and ErC50 were calculated using regression equations. NOEC Confidence limits were calculated using NCSS 2000, and Duncan's Multiple Comparison Test.
Applicant's summary and conclusion
- Conclusions:
- The 72 hour 50% yield inhibition concentration of IN-DCB80 to P. subacapitata is 54.49 mg/L, with 95% confidence limits of 54.063-54.917 mg/L, and a NOEC of 3.5 mg/L. The 72 hour 50% rate inhibition concentration of IN-DCB80 to P. subcapitata is > 120 mg/L, with a NOEC of 6.3 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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