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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: guideline study (with GLP, no analytics)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report date:
1997

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: EG guideline 88/302/EWG (equivalent to OECD209)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Methoxyammonium chloride
EC Number:
209-798-7
EC Name:
Methoxyammonium chloride
Cas Number:
593-56-6
Molecular formula:
CH5NO.ClH
IUPAC Name:
methoxyammonium chloride

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly industrial sewage
Details on inoculum:
Activated sludge from the industrial wastewater treatment plant of BASF, production site Tarragona, Spain.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
30 min

Test conditions

Test temperature:
20 ± 2 °C
pH:
7.0-8.0 (before addition of the sludge sample)
Nominal and measured concentrations:
1000 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- No. of vessels per concentration (replicates; test substance, TS): 1
- No. of vessels per control (replicates; blanc, BC): 3
- No. of vessels per concentration (replicates; positive control, reference substance, RS): 3

TEST MEDIUM / WATER PARAMETERS
- Culture medium: OECD test medium

Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
EC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Relevant effect levels: EC20 (30 min): 8.5 mg/L (nominal); EC50 (30 min): 38 mg/L (nominal).

Applicant's summary and conclusion