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EC number: 439-590-3 | CAS number: 12158-75-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from March 13, 2002 to March 25, 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study (OECD)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 439-590-3
- EC Name:
- -
- Cas Number:
- 12158-75-7
- Molecular formula:
- Cu2H3NO6
- IUPAC Name:
- copper(2+) hydroxide nitrate
- Details on test material:
- batch n°: 060248
solubility in water: 153 mg/L (20°C, based on dissolved copper), 300 mg/L (20°C, based on test substance)
vapour pressure: < 0.00052 Pa at 25°C
percent purity: > 99%
Cu content (measured): 52.6%
storage conditions: ambient temp, dark, dry
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Range-finding test:
Five concentrations of Basic Copper Nitrate were used, spaced by a factor of 10 from 0.01 to 100 mg/L. A stock solution of 300 mg/L was prepared and was diluted in steps of 0.1. From each dilution step, 166.7 mL were added to each reaction vessel and were made up to 500 mL with tap water, synthetic wastewater and sludge. Measurements of oxygen uptake of the activated sludge were performed 30 min. and 3 hours after starting aeration.
Main test:
The main test was performed with 6 concentrations of Basic Copper Nitrate from 300 mg to 9.38 mg/L, spaced by a factor of 0.5, including 2 controls and 3 concentrations of the toxic standard dichlorophenol (5, 15 & 30 mg/L). A stock solution of 528.17 mg/L in tap water was prepared and was diluted by steps by a factor of 2. 284 mL of the solutions were transferred into the test vessels to give the calculated find test concentration after addition of synthetic wastewater, tap water and activated sludge.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Activated sludge from the municipal treatment plant of Pforzheim, Germany was used as a microbial inoculum for the test. This plant predominantly is treating domestic sewage.
2 L of sludge with an initial content of MLSS (mixed liquid suspended solids) of 8 g/L was collected prior one day to the test. It was washed with tap water by centrifugation, resuspended in 4 L of tap water and 200 mL of synthetic wastewater and aerated with an air pump over night.
The MLSS were adjusted to a final concentration of 1.6 g/L in the test medium.
Synthetic water: the test was performed using a synthetic wastewater which was prepared with the following amounts of substances in 1 litre of water:
peptone: 16.0 g
meat extract: 11.0 g
urea: 3.0g
NaCl: 0.7g
CaCl2.2HO: 0.4g
Mg.SO4.7H2O: 0.2g
K2HPO4: 2.8g
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 +/- 2°C
- Nominal and measured concentrations:
- nominal concentrations of copper nitrate (mg/L): 9.38 - 18.75 - 37.5 - 75 - 150 - 300
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 130.34 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Remarks:
- copper nitrate
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The respiration rates were calculated from the slopes of the linear part of the recorder trace as mg O2/L/h between approximately 6.5 mg O2/L and 2.5 mg O2/L over a 10 minutes period.
- Results with reference substance (positive control):
- The EC50 of 3,5-dichlorphenol was in the accepted range 5 to 30 mg/L after 3 hours.
Any other information on results incl. tables
Respiration inhibition in the main test:
30 min. | R | RC1 + RC2 | % inhibition* |
Copper Nitrate (mg/L) | (mgO2/L/h) | (mgO2/L/h) | |
0(1) | 29,038 | 61,855 | 6,11 |
9,38 | 31,14 | 61,855 | -0,68 |
18,75 | 31,57 | 61,855 | -2,13 |
37,5 | 28,60 | 61,855 | 7,52 |
75 | 23,03 | 61,855 | 25,52 |
150 | 17,46 | 61,855 | 43,55 |
300 | 15,88 | 61,855 | 48,67 |
0(2) | 32,817 | 61,855 | -6,11 |
DCP 5 | 23,16 | 61,855 | 25,12 |
DCP 15 | 12,28 | 61,855 | 60,29 |
DCP 30 | 6,33 | 61,855 | 79,53 |
3 hours | R | RC1 + RC2 | % inhibition* |
Copper Nitrate (mg/L) | (mgO2/L/h) | (mgO2/L/h) | |
0(1) | 27,826 | 54,480 | -2,15 |
9,38 | 29,40 | 54,480 | -7,94 |
18,75 | 26,21 | 54,480 | 3,78 |
37,5 | 22,52 | 54,480 | 17,35 |
75 | 14,91 | 54,480 | 45,28 |
150 | 11,57 | 54,480 | 57,53 |
300 | 9,49 | 54,480 | 65,16 |
0(2) | 26,654 | 54,480 | 2,15 |
DCP 5 | 21,94 | 54,480 | 19,46 |
DCP 15 | 9,83 | 54,480 | 63,91 |
DCP 30 | 4,98 | 54,480 | 81,70 |
* a negative value means a value > average of controls.
The inhibition data showed toxic effects of Basic Coppe Nitrate from 37.5 to 300 mg/L after 30 min. and from 18.75 to 300 mg/L after 3 hours.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The 2 control respiration rates are within 15% of each other. The EC50 of 3,5-dichlorophenol was in the accepted range 5 to 30 mg/L after 3 hours.
- Conclusions:
- The effect of Basic Copper Nitrate on a sewage treatment was tested with the activated sludge respiration inhibition test. In a range-finding test which preceeded the main test, effects of the test substance were detected after 3 hours at 100 mg/L. In the main test, which was performed with 6 concentrations between 9.38 and 300 mg/L, spaced by a factor of 2, effects were detected from 18.75 to 300 mg/L after 3 hours. As the effect after 30 min. was < 50% at any test concentration, an EC50 could be calculated only for 3 hours values. It was determined to be 130.34 mg/L test substance (nominal concentration of Copper nitrate in the test solutions).
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