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EC number: 605-717-8 | CAS number: 174333-80-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 217 (Soil Microorganisms: Carbon Transformation Test)
- Deviations:
- yes
- Remarks:
- Test temperature (25 °C) is slightly higher as recommended by OECD guideline (20 ° C +/- 2 °C). Test duration (40d) is longer than recommended by OECD guideline (28d). these deviations are not thought to compromise the test results.
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 and 1000 mg/kg
- Sampling method: On removal from the incubator, the replicates were mixed thorougly and half the quantity was freeze-dried. After freeze-drying three sampßles of each treatment were analysed. - Vehicle:
- yes
- Details on preparation and application of test substrate:
- AMENDMENT OF SOIL
- Type of organic substrate: Reconstituted soil was used comprising a mixture of mature sewage sludge compost (1/3 d.m.) and sandstone (2/3 d.m.). The compost made from a mixture of digested sludge and sawdust was provided by the Sieg-Roche (Canton de Vaud, Switzerland) pilot plant after 9 months of maturation (Hubert 1985) and was mixed with a Permian sandstone without calcium carbonate from the Vosges massif (France).
APPLICATION OF TEST SUBSTANCE TO SOIL
- Method: Test substance was dissolved in ethanol at 0.4 mL/g spiked compost. The dissolved substances were mixed with part of the compost (4g d.m.) nd the ethanol was left to evaporate. The spiked compost was then mixed thoroughly in an incubation cell with the rest of the 60g sample (16g d.m. compost + 40g d.m. sandstone).
VEHICLE:
- Chemical name of vehicle: Ethanol
- Evaporation of vehicle before use: Yes - Test organisms (inoculum):
- other: aged mixture of sewage sludge compost and sandstone
- Total exposure duration:
- 40 d
- Test temperature:
- 25 °C
- Moisture:
- 60% water-holding capacity
- Details on test conditions:
- TEST SYSTEM
- Test container (type, material, size): Incubation cell in a multipurpose assembly incubator ( with intermittent aeration)
- No. of replicates per concentration: 1 (triplicates were mixed on removal from test cells)
- No. of replicates per vehicle control: 1 (triplicates were mixed on removal from test cells)
- Other: Incubated in darkness.
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographical reference of sampling site (latitude, longitude): Reconstituted soil was used comprising a mixture of mature sewage sludge compost (1/3 d.m.) and sandstone (2/3 d.m.). The compost made from a mixture of digested sludge and sawdust was provided by the Sieg-Roche (Canton de Vaud, Switzerland) pilot plant after 9 months of maturation and was mixed with a Permian sandstone without calcium carbonate from the Vosges massif (France).
- Soil texture of the sandstone
- % sand: 75%
- % silt: 12%
- % clay: 13%
- pH (in water): 6.80 (mixed soil)
- C:N ratio: 20 (mixed soil)
Total P: 1% (mixed soil)
- Maximum water holding capacity (in % dry weight): 51% WHC of mixed soil and adjusted to 60% WHC for the test
- Cation exchange capacity (mmol/kg): 22.1 meq/100g of the mixed soil
EFFECT PARAMETERS MEASURED: Respiration (CO2 evolution) was recorded daily throughout the test period of 40 days. The cells were flushed periodically with a stream of CO2-free air to remove accumulated CO2 and restore the O2 content, the effluent air being bubbled in 15 mL 0.1 N NaOH. The absorbed CO2 was determined by a conductivity method.
VEHICLE CONTROL PERFORMED: yes - Nominal and measured concentrations:
- Nominal: 100 and 1000 ppm
- Reference substance (positive control):
- no
- Key result
- Duration:
- 40 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- respiration rate
- Details on results:
- Respiration started at the same rate (37 µg C/g organic C per h) in control and treatments. The rates then decreased progressively, indicating depletion of easily mineralizable C and N. CO2 evolution did not differ significantly between the 100 ppm samples and the controls. In contrast, in 1000 ppm samples, CO2 evolution was significantly depressed by the 4th day (F< 0.05). Respiration was depressed by an average o f 64% compared with controls between the 5th and the 40th days.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a long-term toxicity test performed similar to OECD guideline 217, a 40d-NOEC value of 100 mg/kg dw was measured for inhibition of microbial respiration of a mixture of sewage sludge compost and sandstone.
- Executive summary:
In a long-term toxicity test performed similar to OECD guideline 217, soil microbes were incubated with 100 and 1000 ppm branched 4-nonylphenol (NP) over an exposure time of 40 days. An aged mixture of sewage sludge compost and sandstone was used as inoculum. Respiration (CO2 evolution) was recorded daily throughout the test period. Respiration started at the same rate (37 µg C/g organic C per h) in control and treatments. The rates then decreased progressively, indicating depletion of easily mineralizable C and N. CO2 evolution did not differ significantly between the 100 ppm samples and the controls. In contrast, in 1000 ppm samples, CO2 evolution was significantly depressed by the 4th day. Thus, the 40d-NOEC value is determined to be 100 mg/kg dw.
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Analogue justification attached (chapter 13).
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 40 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- respiration rate
Referenceopen allclose all
Description of key information
NOEC (40d) = 100 mg/kg (nominal) for respiration inhibition of mixture of sewage sludge compost and sandstone (read across)
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 100 mg/kg soil dw
Additional information
Since no study assessing the toxicity of Benzaldehyde, 2-hydroxy-5-nonyl, oxime, branched (CAS 174333-80-3) to soil microorganisms is available, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 Grouping of substances, a read-across to Phenol, 4-nonyl-,branched was conducted, which is a secondary component and structurally similar to the main component of the substance. The only structural difference between the source substance and the target substance is the lack of an aldoxime group at the phenol ring of the molecule.
The read across is justified due to the following reasons:
1) The similarity of structure and functional groups and accordingly similar physico-chemical properties result in similar environmental behavior and fate.
2) Based on a profound ecotoxicological data set available for Phenol, 4-nonyl-,branched it can be concluded that Phenol, 4-nonyl-,branched exhibit an equal to higher aquatic toxicity compared to Benzaldehyde, 2-hydroxy-5-nonyl, oxime, branched (see table below). Therefore, using data from Phenol, 4-nonyl-,branched to assess the intrinsic hazard of Benzaldehyde, 2-hydroxy-5-nonyl, oxime, branched is considered to be a very conservative approach (worst case scenario).
Substance |
Benzaldehyde, 2-hydroxy-5-nonyl, oxime, branched |
Phenol, 4-nonyl, branched* |
CAS number |
174333-80-3 |
84852-15-3 |
Structure |
see attachment (chapter 6.3) |
see attachment (chapter 6.3) |
Molecular formula |
C16O2NH25 |
C15H24O |
Molecular weight |
~ 263 g/mole |
~ 220.35 g/mole |
PC parameter |
|
|
Water solubility |
0.4 mg/L (EU method A.6) |
5.7 mg/L (ASTM E 1148-02) |
Partition coefficient |
5.5 (EU method A.8) |
5.4 (OECD 117) |
Vapour pressure |
0.37 Pa at 20 °C (OECD 104) |
~1 Pa at 20 °C (ASTM-D 2879) |
Environmental fate |
|
|
Biodegradability |
0 % in 28 days (OECD 302c) |
non-adapted inoculum: 0 % in 28 days (OECD 301B)
adapted inoculum: 48.2-62 % in 28 days (OECD 301B) |
Adsorption [log KOC] |
3.7 (OECD 121) |
4.35 - 5.69 (EPA OTS 796.2750) |
Hydrolysis |
not relevant |
|
Ecotoxicology |
|
|
Short-term toxicity to fish [96h-LC50] |
1.1 mg/L (EU method C.1) |
0.05 – 0.22 mg/L (different methods) |
Long-term toxicity to aquatic invertebrates [NOEC] |
0.005 mg/L (OECD 210) |
0.006 mg/L (ASTM E 1241-05) |
Short-term toxicity to aquatic invertebrates [48h-EC50] |
2.7 mg/L (EU method C.2) |
0.08 – 0.14 mg/L (different methods) |
Long-term toxicity to aquatic invertebrates [21d-NOEC] |
0.189 mg/L (OECD 211) |
0.024 - 0.116 mg/L (different methods) |
Short-term toxicity to algae [72h-EC50] |
36.3 mg/L (OECD 201) |
0.33 - 1.3 mg/L (different methods) |
Long-term toxicity to algae [72h-NOEC/EC10] |
14.9 mg/L (OECD 201) |
0.5 mg/L (Algal growth inhibition test according to UBA 1984) |
Toxicity to microorganisms [EC50] |
200.4 mg/L (OECD 209) |
950 mg/L (OECD 209) |
* Data were taken from Phenol, 4-nonyl-,branched (CAS 84852-15-3) dossier published on the ECHA data base
In a long-term toxicity test performed similar to OECD guideline 217, soil microbes were incubated with 100 and 1000 ppm branched 4-nonylphenol (NP) over an exposure time of 40 days (Trocme et al. 1988). An aged mixture of sewage sludge compost and sandstone was used as inoculum. Respiration (CO2 evolution) was recorded daily throughout the test period. Respiration started at the same rate (37 µg C/g organic C per h) in control and treatments. The rates then decreased progressively, indicating depletion of easily mineralizable C and N. CO2 evolution did not differ significantly between the 100 ppm samples and the controls. In contrast, in 1000 ppm samples, CO2 evolution was significantly depressed by the 4th day. Thus, the 40d-NOEC value is determined to be 100 mg/kg dw. Based on the reasons given above this value is considered to be sufficiently conservative to be used for the hazard assessment of Benzaldehyde, 2-hydroxy-5-nonyl, oxime, branched (CAS 174333-80-3).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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