N-methylaniline

Hydrolysis

Administrative data

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Study well documented, meets generally accepted principles of measurement of biodegradation using a method different from those recommended by international guidelines.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1986

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

yes

Remarks:

Use of an automated headspace method.

Principles of method if other than guideline:

A chemical control was tested simultaneously in a biodegradation test with activated sludge to determine degradation in the absence of inoculum. In fact this control evaluates the hydrolytical stability of the test substance under the test conditions applied.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

N-methylaniline

EC Number:

202-870-9

EC Name:

N-methylaniline

Cas Number:

100-61-8

Molecular formula:

C7H9N

IUPAC Name:

N-methylaniline

Radiolabelling:

no

Study design

Analytical monitoring:

yes

Details on sampling:

Samples had been acidified by injection of 0.5 ml 2 N phosphoric acid and filtered through 0.22-pm membrane filters prior to analysis.

Buffers:

Not applicable.

Estimation method (if used):

Not applicable.

Duration of test

Duration:

21 d

Initial conc. measured:

20 mg/L

Number of replicates:

3 for each time point.

Positive controls:

no

Negative controls:

no

Statistical methods:

Not applied.

Results and discussion

Preliminary study:

Not applicable.

Test performance:

The results refer to abiotic degradation, which may include hydrolysis, but determaination of hydrolysis was not the ultimate endpoint of the experiment.

Transformation products:

no

Total recovery of test substance (in %)

% Recovery:

ca. 92

pH:

7

Temp.:

25 °C

Duration:

21 d

Dissipation DT50 of parent compound

Key result

pH:

7

Temp.:

25 °C

Remarks on result:

other: the test substance was not degraded under the test conditions

Applicant's summary and conclusion

Conclusions:

The test substance showed no significant abiotic degradation in water during 21 days of exposure at pH of 7.0 ± 0.2 and a temperature of 25 ± 2 °C.