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EC number: 235-837-2 | CAS number: 13001-46-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Carbon disulphide is both a reagent in the manufacture, as well as a decomposition product of xanthates. Potassium isobutyl xanthate readily decomposes to carbon disulphide, especially in the presence of moisture/water. Therefore, the health effects of carbon disulphide (CS2) need to be considered in the assessment of Potassium isobutyl xanthate.
Data source
Reference
- Reference Type:
- publication
- Title:
- Carbon disulphide induced impairments in male reproductive system in rats.
- Author:
- Patel KG, Gautam AK, and Vaghasia YV
- Year:
- 1 999
- Bibliographic source:
- Ind. J. Physiol. & Allied Sci., 53(1): 22-28
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- CS2 was administer intraperitoneally to male rats in order to examine alterations in reproductive hormones, testicular tissue and epididymis.
- GLP compliance:
- not specified
- Type of method:
- in vivo
Test material
- Reference substance name:
- Carbon disulphide
- EC Number:
- 200-843-6
- EC Name:
- Carbon disulphide
- Cas Number:
- 75-15-0
- Molecular formula:
- CS2
- IUPAC Name:
- dithioxomethane
- Test material form:
- solid: compact
- Details on test material:
- Carbon disulphide is both a reagent in the manufacture, as well as a decomposition product of xanthates. Potassium isobutyl xanthate readily decomposes to carbon disulphide, especially in the presence of moisture/water. Therefore, the health effects of carbon disulphide (CS2) need to be considered in the assessment of Potassium isobutyl xanthate.
- Name of test material (as cited in study report): carbon disulfide
- Molecular formula (if other than submission substance): CS2
- Physical state: liquid
- Analytical purity: 99.99%
- Stability under test conditions: yes
- Storage condition of test material: temperature room
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Charles-Foster
- Sex:
- male
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- cotton seed oil
- Details on exposure:
- Different dosages of CS2 dissolved in maruti micro reined cotton seed oil viz. 25,50,100 and 200 mg/kg body weight daily were administered intraperitoneally over a period of 30 days
- Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 30 days
- Frequency of treatment:
- daily
- Duration of test:
- 30 days
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 25, 50, 100 mg
Basis:
nominal conc.
- No. of animals per sex per dose:
- 10
- Control animals:
- yes, concurrent no treatment
- yes, concurrent vehicle
- Details on study design:
- Different dosages of CS2 dissolved in maruti micro reined cotton seed oil viz. 25,50,100 and 200 mg/kg body weight daily were administered intraperitoneally over a period of 30 days. Pathomorphological changes and functional impairments were observed in male reproductive organs. Significant decrease in serum testosterone levels and marked degenerative changes in testicular tissue were observed specially in 100 and 200 mg/kg CS2 treated rats.
Results and discussion
Effect levels
- Dose descriptor:
- LOAEC
- Effect level:
- 25 mg/kg bw/day
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- other: decreased serum testosterone levels, histologic findings
Observed effects
Any other information on results incl. tables
Table 1: Effects of different doses of CS2 on serum testosterone levels
and tissue weights of rats.
Dose (mg/kg bw) |
Testosterone (ng/dl) |
Testis (mg) |
Epididymis (mg) |
Control |
540±37.32 |
1.35±0.08 |
33±2 |
Vehicle control |
504.5±52.22 |
1.32±0.07 |
36.4±1.4 |
25 |
224.3±17.35* |
1.27±0.07 |
35.3±2.2 |
50 |
207.77±23.32* |
1.32±0.13 |
33.2±2.3 |
100 |
84±29.88* |
1.37±0.12 |
32.7±2.4 |
200 |
31.6±3.09* |
1.34±0.06 |
33.3±0.3 |
*p < 0.001
CS2 exposure exerted reductions in body weights, that were remarkable after treatment with the 2 highest doses. Exposure to all concentrations resulted in a significant dose-dependent decline in serum testorone levels, while no alterations were recorded in the testicular and epididymal weights. The histologic examinations revealed no changes in the epididymis. The testis were evidently affected with ' thickening of peritubular membrane, rupturing of seminiferous basement membrane, degeneration and disorganization of spermatogonial cells and less number or absence of sperms in the lumen'. Such effects were much more pronounced at the last two groups.
Applicant's summary and conclusion
- Conclusions:
- The present observations clearly indicate effects of CS2 on the male reproduction system. Nonetheless, the relevance of the study is questionable due to the invasive route of exposure.
Carbon disulphide is both a reagent in the manufacture, as well as a decomposition product of xanthates. Potassium isobutyl xanthate readily decomposes to carbon disulphide, especially in the presence of moisture/water. Therefore, the health effects of carbon disulphide (CS2) need to be considered in the assessment of Potassium isobutyl xanthate. - Executive summary:
Deleterious effects of short term exposure of CS2 were investigated on reproductive organs of male albino rats. Different dosages of CS2 dissolved in maruti micro reined cotton seed oil viz. 25,50,100 and 200 mg/kg body weight daily were administered intraperitoneally over a period of 30 days. Pathomorphological changes and functional impairments were observed in male reproductive organs. Significant decrease in serum testosterone levels and marked degenerative changes in testicular tissue were observed specially in 100 and 200 mg/kg CS2 treated rats. Diminution of serum testosterone levels and degeneration in Leydig ceils indicate a definite alterations in the process of steroidogenesis after CS2treatment. Pronounced changes in testicular structure indicated plausible effect on spermatogenesis. Further, androgenic deficiency, evident by decrease in testosterone level after CS2 treatment produced alterations in epididymis.
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