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EC number: 244-599-9 | CAS number: 21829-50-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The 72-h EC50 value and the 72-h NOEC for monoethanolamine in Pseudokirchneriella subcapitata are 2.5 and 1 mg/L respectively. The 3-h toxicity threshold (equivalent to an EC3) for citric acid in Scenedesmus quadricauda is 640 mg/L.
After correction for molecular weight the effect concentrations for tris[(2-hydroxyethyl)ammonium] citrate are 15.4, 6.15 and 1,250 mg/L respectively. The values of 10.4 and 4.14 mg/Lwill be used in the assessment.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 15.4 mg/L
- EC10 or NOEC for freshwater algae:
- 6.15 mg/L
Additional information
No studies with algae are available for tris[(2-hydroxyethyl)ammonium] citrate. Tris[(2-hydroxyethyl)ammonium] citrate is the neutral salt of monoethanolamine and citric acid. Monoethanolamine and citric acid are the starting materials for the synthesis of tris[(2-hydroxyethyl)ammonium] citrate, and the only difference is that in tris[(2-hydroxyethyl)ammonium] citrate the substances are present in their ionic forms as a monoethanolamine cation and a citrate anion. It is expected that the ecotoxicological properties of tris[(2-hydroxyethyl)ammonium] citrate shall be governed by the properties of monoethanolamine and citric acid (for more details see reporting format for the analogue approach in Appendix A.2).
A GLP-compliant OECD 201 test with Pseudokirchneriella subcapitata is available for monoethanolamine (NITE, 1996b). The72-h EC50 value and the 72-h NOEC for monoethanolamine are 2.5 and 1 mg/L respectively, a safety factor need not to be applied to these values. Instead, the effect concentration will be corrected for molecular weight. The effect concentrations for tris[(2-hydroxyethyl)ammonium] citrate are 15.4 and 6.15 mg/L.
For citric acid a non-GLP growth inhibition study with Scenedesmus quadricauda is available (Bringmann and Kühn, 1977). The 8-d toxicity threshold (equivalent to an EC3 value) is 640 mg/L, a safety factor need not to be applied to this value. Instead, the effect concentration will be corrected for molecular weight. The effect concentration for tris[(2-hydroxyethyl)ammonium] citrate is 1,250 mg/L.
The effect concentrations of 10.4 and 4.14 mg/L will be used in the assessment.
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