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EC number: 248-211-9 | CAS number: 27076-30-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993-03-08 to 1993-04-07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study is an OECD 401 study under GLP and well documented
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- ACMEO
- IUPAC Name:
- ACMEO
- Reference substance name:
- 3-[(2-hydroxyethyl)amino]propionamide
- EC Number:
- 248-211-9
- EC Name:
- 3-[(2-hydroxyethyl)amino]propionamide
- Cas Number:
- 27076-30-8
- Molecular formula:
- C5H12N2O2
- IUPAC Name:
- N~3~-(2-hydroxyethyl)-beta-alaninamide
- Test material form:
- other: colourless slightly viscous liquid
- Details on test material:
- - Name of test material (as cited in study report): ACMEO
- Physical state: colourless slightly viscous liquid
- Analytical purity: 58% aqueous solution
- Lot/batch No.: 3S1005
- Storage condition of test material: +4°C
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Spraque Sawley strain rats. Weight at start:
TEST ANIMALS
- Source: supplied by Charles River (UK) Ltd.
- Age at study initiation: approx. five to eight weeks
- Weight at study initiation: males 156-188 g, females 142-154 g
- Fasting period before study: overnight fast immediately before dosing
- Housing: in groups of five by sex in solid polypropylene cages with sawdust bedding
- Diet (e.g. ad libitum): yes
- Water (e.g. ad libitum): yes
- Acclimation period: minimum 5 day period
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-22°C
- Humidity (%): 42- 54 %
- Air changes (per hr): approx. 15/ hr
- Photoperiod (hrs dark / hrs light): 12 hours light, 12 hours dark
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- all animals (5/sex) were dosed once only with the undiluted test substance by oral gavage using a metal cannula. The volume administered to each animal was calculated according to its fasted body weight at the time of dosing (Specific gravity: 1.129; dose volume 1.78 mL/kg)
- Doses:
- 2000 mg/kg body weight
- No. of animals per sex per dose:
- 5/ sex /dose
- Control animals:
- no
- Details on study design:
- A range finding study was performed with one male and one female at a dose of 2000 mg/kg body weight to establish a dosing regime. The animals were observed for deaths or overt signs of toxicity 0.5, 1, 2 and 4 hours after dosing and subsequent once daily for 5 days.
Based on the results of the range finding study in the main study all animals (5/sex) were dosed once only by gavage at a dose of 2000 mg/kg body weight using a metal cannula. The volume administered to each animal was calculated according to its fasted body weight at the time of dosing. The animals were observed for deaths or overt signs of toxicity 0.5, 1, 2 and 4 hours after dosing and subsequent once daily for 14 days. Individual bodyweights were recorded prior to dosing on day 0 and on Days 7 and 14. At the end of the study the animals were killed by cervical dislocation and subjected to gross pathological examination. No tissues were retained.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortality occurred.
- Clinical signs:
- other: No signs of systemic toxicity were noted during the study
- Gross pathology:
- No abnormalities were noted at necropsy.
Applicant's summary and conclusion
- Interpretation of results:
- practically nontoxic
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The acute oral median lethal dose (LD 50) of the test material ACMEO in the spraque Dawley strain rat was found to be greater than 2000 mg/kg body weight.
- Executive summary:
The acute oral toxicity of ACMEO was tested in 10 Spraque Dawley strain rat (5/sex) according to OECD test guideline 401. The test substance was applied once by oral gavage undiluted at a dose level of 2000 mg/kg body weight (limit test). Symptoms were recorded dayily and the animals were weight once a week upon an observation period of 14 days. No lethality occurred during the subsequent observation period. The animals killed at the end of the observation period showed no macroscopically visible changes.
Based on the study results the acute oral median lethal dose (LD 50) of the test material ACMEO in the spraque Dawley strain rat was found to be greater than 2000 mg/kg body weight.
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