Pent-3-enenitrile

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Adopted April 13, 2004.
In addition, the following guidance document was followed:
¿ Guidance document on aqueous-phase aquatic toxicity testing of difficult test chemicals,
OECD series on testing and assessment number 23, 2018.

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Samples for possible analysis were taken from all test concentrations and the control
according to the schedule below.
Frequency: at t=0 h and t=48 h
Volume: 2.0 mL from the approximate centre of the test vessels
Storage: Samples were stored in a freezer (=-15°C) until analysis
at the analytical laboratory of the Test Facility.

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

The hardness of test medium expressed as CaCO3: 180 mg/L with a
pH between 6 and 9

Test temperature:

19-20 degr C

pH:

7.8

Dissolved oxygen:

8.2-9.0 mg/L

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: In conclusion, the 48h-EC50 for Daphnia magna exposed to 3-PENTENENITRILE was beyond the range of concentrations tested, i.e. exceeded the analytically confirmed regulatory limit concentration of 100 mg/L

Results with reference substance (positive control):

The 24h-EC50 in this reference test with K2Cr2O7 was within the expected range of 0.6 and
2.1 mg/L as specified in ISO International Standard 6341, October 2012. The 48h-EC50 was
within the expected range of 0.28 and 0.75 mg/L, which is based on reference tests performed
at the Test Facility during the last ten years.
The 24h-EC50 was 0.92 mg/L with a 95% confidence interval between 0.78 and 1.1 mg/L.
The 48h-EC50 was 0.55 mg/L with a 95% confidence interval between 0.47 and 0.64 mg/L.
In conclusion, the sensitivity of this batch of Daphnia magna was in agreement with ISO
International Standard 6341, October 2012 and the historical data collected at Charles River
Den Bosch.

Validity criteria fulfilled:

yes

Conclusions:

In conclusion, the 48h-EC50 for Daphnia magna exposed to 3-PENTENENITRILE was
beyond the range of concentrations tested, i.e. exceeded the analytically confirmed regulatory
limit concentration of 100 mg/L.

Description of key information

Based on reliable data from closed vessel study, after 48 hours of exposure to the test substance of daphnids a EC50 = > 100 mg/L was detrmined.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L