Registration Dossier
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EC number: 261-619-1 | CAS number: 59130-69-7
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
No toxic effects up to the limit of water solubility (< 0.05 mg/L) for for Danio rerio (OECD 203)
Key value for chemical safety assessment
Additional information
No studies investigating the short-term toxicity of Hexadecyl 2-ethylhexanoate (CAS No. 59130-69-7) to freshwater fish are available. Therefore, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 a read-across to the structurally related source substance Hexanoic acid, 2-ethyl-, C16-18 alkyl esters (CAS No. 90411-68-0) is applied.
A read across from Hexanoic acid, 2-ethyl-, C16-18 alkyl esters (CAS No. 90411-68-0) to Hexadecyl 2-ethylhexanoate (CAS No. 59130-69-7) to cover the short-term toxicity to freshwater fish endpoint is acceptable as first, both substances have common functional groups being esters of 2-ethylhexanoic acid and fatty alcohols with carbon chain lengths of C16 - C18 and C14 - C18, respectively. Second, both substances have the common precursor 2-ethylhexanoic acid and fatty alcohol and common breakdown products as both are expected to be rapidly metabolised in the body via enzymatic hydrolysis to the starting materials (e.g., see Barron et al. 1999 for metabolism of esters in fish), which then fed into body’s natural catabolic processes, such as ß-oxidation and citric acid cycle (e.g., Rahn et al., 1973; Henderson, 1996).
Based on their structural similarity the physico-chemical properties are also similar. Both substances are insoluble in water (< detection limit of used method), feature similar partition coefficients of 5.82 (CAS No. 90411-68-0) and 6.15 (CAS No. 59130-69-7) and the main components of the UVCB substances have similar molecular weights of 368.6 - 396.7 (CAS No. 90411-68-0) and 368.6 (CAS No. 59130-69-7). The volatility of both substances is low (< 0.01 Pa at 20 °C). Hence, the behaviour, the fate, the bioaccumulation and thus the toxicity of the substances in the aquatic environment are considered to be identical.
The study with Hexanoic acid, 2-ethyl-, C16-18 alkyl esters (CAS No. 90411-68-0) was conducted under static conditions according to OECD guideline 203 using Danio rerio as test organism (Bouillon, 2012); however, was not performed under GLP conditions. A 100 mg/L loading rate WAF was tested in a limit test. No mortality was observed in the treatment and the control throughout the test period of 96 h. Hence, a 96 h-LL50 is determined to be > 100 mg/L based on the nominal test concentration.
Due to structural and property similarities to the analogue substance, Hexanoic acid, 2-ethyl-, C16-18 alkyl esters (CAS No. 90411-68-0), as explained above, this results is also applicable to read across to Hexadecyl 2-ethylhexanoate (CAS No. 59130-69-7).
A detailed reference list is provided in the technical dossier (see IUCLID, section 13) and within the CSR.
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