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Diss Factsheets
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EC number: 249-535-3 | CAS number: 29253-36-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Bis(isopropyl)naphthalene
- EC Number:
- 254-052-6
- EC Name:
- Bis(isopropyl)naphthalene
- Cas Number:
- 38640-62-9
- Molecular formula:
- C16H20
- IUPAC Name:
- Bis(isopropyl)naphthalene
- Details on test material:
- - Name of test material (as cited in study report): KMC
- Physical state: colorless and transparent oily liquid
- Analytical purity: ≥ 99.5%
- Isomers composition: no data
- Lot/batch No.: TR-
- Stability under test conditions: more than 1 year at room temperature
- Storage condition of test material: no data
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Crjh : CD
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Japan Inc.
- Age at study initiation: no data
- Weight at study initiation: males 115 - 166 g, females 102 - 127 g
- Fasting period before study: yes
- Housing: 5 per cage in polycarbonate cages
- Diet (e.g. ad libitum): pelletized rat diet (rat.MF, Oriental Yeast Co.), ad libitum
- Water (e.g. ad libitum): tap water through polycarbonate bottles, ad libitum
- Acclimation period: more than a week
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 - 24
- Humidity (%): 50 - 60
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): no data
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- olive oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 0.18 - 0.7 mL TS/mL olive oil according to dose
- Amount of vehicle (if gavage): 1 mL/100 g bw
- Justification for choice of vehicle: no data
MAXIMUM DOSE VOLUME APPLIED: 1 mL/100 g bw - Doses:
- males: 1.73, 2.21, 2.78, 3.46, 4.32, 5.38, and 6.72 g/kg bw (1.8, 2.3, 2.9, 3.6, 4.5, 5.6, 7.0 mL/kg)
females: 2.78, 3.46, 4.32, 5.38, 6.72 (2.9, 3.6, 4.5, 5.6, 7.0 mL/kg) - No. of animals per sex per dose:
- 10
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: several times at day of administration, once or more times daily thereafter until 14 days
(time of sacrifice). Body weights were measured shortly before, 7 and 14 days after administration.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Statistics:
- LD50 values were determined using the method of Litchfield and Wilcoxon.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 4 130 mg/kg bw
- 95% CL:
- 3 550 - 4 800
- Remarks on result:
- other: LD50 is reported as 4.3 mL/kg bw
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 4 320 mg/kg bw
- 95% CL:
- 3 650 - 5 090
- Remarks on result:
- other: LD50 is reported as 4.5 mL/kg bw
- Mortality:
- 1.58 g/kg group: 0/10
2.20 g/kg group: 1/10 (day 2)
2.78 g/kg group: 1/20 (day 3)
3.46 g/kg group: 6/20 (days 2-4)
4.32 g/kg group: 11/20 (days 1-5)
5.38 g/kg group: 14/20 (days 1-5)
6.72 g/kg group: 20/20 (days 1-6) - Clinical signs:
- other: Reduced activity, reduced muscle tonus, toe walking, diarrhea, incontinence, haemic nasal discharge, emaciation
- Gross pathology:
- Dead animals
Dose-related discolored liver, discolored and atrophic spleen and kidneys, reddish purple appearance of the adrenal glands, hypertrophy of the adrenal glands, scattered reddish spots in and reddish appearance of the lungs, hemorrhage in the thymus gland
Surviving animals
Occasionally in male, but not in female animals, scattered reddish spots in the lung, atrophy of spleen and hypertrophy of mesentery lymph nodes.
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- In a valid acute oral toxicity study according to OECD test guideline 401, LD50 values of 4130 and 4320 mg/kg bw were determined for male and female rats respectively.
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