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EC number: 500-446-0 | CAS number: 160901-09-7 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
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- Partition coefficient
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- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
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- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
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- Dissociation constant
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
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- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
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- Endpoint summary
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 Aug 2020 - 15 Apr 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations
- Sampling method: sampling at 0h and 24 (fresh solutions) and after 24 and 48 h (aged solutions)
- Sample storage conditions before analysis: The samples were mixed with 10 mL acetonitrile and stored deep-frozen (≤ - 18 °C) until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution with a nominal concentration of 25 mg/L was prepared by adding the necessary amount of test item to a volumetric flask. Test medium was added and the solution was homogenised by shaking. The solution was observed to be clear and transparent; foam was observed. Test item concentrations were prepared by dilution with test medium. Test solutions were observed to be clear and transparent. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Clone V
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: Daphnia magna was bred as single culture (1 daphnid per 100 mL) in Elendt M4 medium. pH-value: 6.0 – 9.0, dissolved oxygen: > 60 % saturation, total hardness 140 - 250 mg/L (as CaCO3), fed with single cell green algae (Desmodesmus subspicatus, formerly Scenedesmus subspicatus) at least three times a week.
- Source: bred at testing faciltiy, originally obtained from the Federal Environment Agency in Berlin/Germany.
- Feeding during test: none
ACCLIMATION
- Acclimation period: none - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 232 mg/L CaCO3
- Test temperature:
- 19.8 - 20.4 °C
- pH:
- 7.78 – 8.08
- Dissolved oxygen:
- 8.74 ± 0.17 mg/L
- Nominal and measured concentrations:
- Nominal concentrations of 5.00, 2.50, 1.25, 0.625, 0.313 mg/L
Time weighted mean concentrations: 3.62, 1.54, 0.753, 0.366, 0.183 mg/L (based on 3 representative constituents) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers (nominal: 100 mL)
- Type (delete if not applicable): covered by glass plate
- Volume of solution: ≥ 50 mL
- Aeration: none
- Renewal rate of test solution (frequency): after 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Loading rate: ≥ 10 mL / animal
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: Temperature, pH-value and oxygen concentration were measured after 0, 24 hours (aged and fresh) and 48 hours
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light and 8 hours darkness
- Light intensity: 903-1443 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mobility and behavioural changes at 24 and 48 h
RANGE-FINDING STUDY
- Test concentrations: 0.050, 0.50, 5.00, 50.0 mg/L
- Results used to determine the conditions for the definitive study: EC50 (48 h) >0.50 mg/L < 5 mg/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.95 mg/L
- 95% CI:
- >= 1.67 - <= 2.29
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: based on three representative constituents
- Remarks:
- C10EO2, C10EO3, C10EO6
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.753 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: based on three representative constituents
- Remarks:
- C10EO2, C10EO3, C10EO6
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control:
no
- Other adverse effects control: After 48 hours two mobile daphnids in the control group and one mobile daphnid at the test item concentration 0.313 mg/L were observed as being stuck at the surface.
The appearance of test solutions was clear and transparent in all nominal test item concentrations at all observations. - Results with reference substance (positive control):
- EC50 (24 h) of the reference item potassium dichromate: 0.6 - 2.1 mg/L (test started on 18 Aug 2020).
- Reported statistics and error estimates:
- The NOEC was determined based on the highest test concentration at which no significant immobilisation was determined after 24 and 48 hours test duration.
The EC50 after 24 was not determined statistically. The 48 hours EC50 was determined by trimmed Spearman-Karber procedure. - Validity criteria fulfilled:
- yes
- Remarks:
- Please see table 1 at "any other information on results incl. tables"
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 26 Oct - 28 Oct 1984
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The acute toxicity to Daphnia magna was determined under static conditions.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone
- Concentration of vehicle in test medium (final test solution): 0.1 ml/L - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: Laboratory culture obtained from ICI Brixham Laboratory; original source: I.R.Ch.A., France
- Age at study initiation (mean and range, SD): < 24 h
CULTURE
- Culture conditions: glass vessels (0.5 - 4 L) containing reconstituted fresh water. New cultures are started with animals < 24 h old, at a density of about 20 per litre. Cultures are held in a temperature controlled room at 18 – 22 °C and artificial light in a 16 h light/8 h dark cycle. The water in the vessels is renewed weekly and the cultures are discarded when they are 5 weeks old or if there appear ephippia.
- Type and amount of food: Chlorella vulgaris, approx. 1E06 cells/mL in the Daphnia vessels
- Feeding frequency: daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 160 mg/L as CaCO3
- Test temperature:
- 18 - 22 °C
- pH:
- 8.2 - 8.3
- Dissolved oxygen:
- 8.5 - 9.2 mg/L
- Nominal and measured concentrations:
- Nominal: 0.5, 1.1, 2.3, 4.8, 10, 22, 47, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Crystallising dishes
- Material, fill volume: glass, fill vol. 150 mL
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per vehicle control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted fresh water containing 192 mg/L NaHCO3, 120 mg/L CaSO4*2H2O, 120 mg/L MgSO4, 8 mg/L KCl
- Aeration: Test water was aerated 24 h before use and was assumed to be saturated with dissolved oxygen
- Ca/Mg ratio: 1:1
- Culture medium different from test medium: no
- Intervals of water quality measurement: Monitoring of temperature in one test vessel every 4 h, pH and dissolved oxygen was measured in one control and the highest test concentration at test start and test end.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobilisation was recorded after 24 and 48 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. 2.0 - Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.5 mg/L
- 95% CI:
- >= 2.1 - <= 2.9
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- not specified
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 26 Oct - 30 Oct 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: Guidelines issued by the Ministry of Agriculture, Fisheries and Food, Burnham-on-Crouch, UK
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dispersion in water - Test organisms (species):
- Crangon crangon
- Details on test organisms:
- TEST ORGANISM
- Common name: Brown shrimp
- Source: P. Garnett, King's Lynn, Norfolk, UK
- Weight at study initiation (mean and range, SD): mean 1.32 g, SD 0.41
- Length at study initiation (length definition, mean, range and SD): mean 4.5 cm, SD 0.6
ACCLIMATION
- Acclimation period: 19 Oct - 26 Oct 1989
- Acclimation conditions (same as test or not): 14 °C, dissolved oxygen ≥ 8.1 mg/L
- Health during acclimation (any mortality observed): < 5% in 4 days prior to test - Test type:
- semi-static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 14 °C
- Nominal and measured concentrations:
- Nominal: 1.8, 3.2, 5.6, 10 and 18 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: aquarium
- Material, size, headspace, fill volume: glass, fill volume 20 L
- Aeration: Via narrow bore glas tubes
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1.3 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic sea water at 32 o/oo S
- Aeration: ≥ 12 h prior to use
OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality and abnormal behaviour was recorded after 3, 6, 24, 48, 72 and 96 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8 - Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 9.9 mg/L
- 95% CI:
- >= 8.9 - <= 11
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Behavioural abnormalities: Lethargy was observed at 10 mg/L
- Mortality of control: no mortality and no abnormality - Validity criteria fulfilled:
- not specified
Referenceopen allclose all
Table 1: Validity criteria for OECD 202 (2004)
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0% |
yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
≥ 8.2 mg/L |
yes |
Table 2: Results of the test, 48 h values
|
TWA [mg/L] |
|||||
|
Control |
0.183 |
0.366 |
0.753 |
2.54 |
3.62 |
|
Immobilised daphnids after 48 h |
|||||
Replicate 1 |
0 |
0 |
0 |
0 |
0 |
5 |
Replicate 2 |
0 |
0 |
1 |
0 |
0 |
5 |
Replicate 3 |
0 |
0 |
0 |
0 |
2 |
5 |
Replicate 4 |
0 |
1 |
0 |
0 |
1 |
5 |
Σ |
0 |
1 |
1 |
0 |
3 |
20 |
% |
0 |
5 |
5 |
0 |
15 |
100 |
24 h EC50 value: 4.8 mg/L, 95% CL = 4.0 - 5.8 mg/L
Table: Immobilisation of D. magna
Concentration of test substance (mg/L) |
Number of D. magna |
Number immobilised |
|
24 h |
48 h |
||
Control |
10 |
0 |
0 |
Control |
10 |
0 |
0 |
Control |
10 |
0 |
0 |
0.5 |
10 |
0 |
0 |
0.5 |
10 |
0 |
0 |
0.5 |
10 |
0 |
0 |
1.1 |
10 |
0 |
2 |
1.1 |
10 |
0 |
0 |
1.1 |
10 |
0 |
0 |
2.3 |
10 |
1 |
3 |
2.3 |
10 |
2 |
4 |
2.3 |
10 |
1 |
5 |
4.8 |
10 |
4 |
8 |
4.8 |
10 |
6 |
10 |
4.8 |
10 |
6 |
10 |
10 |
10 |
9 |
10 |
10 |
10 |
8 |
10 |
10 |
10 |
8 |
10 |
22 |
10 |
10 |
10 |
22 |
10 |
10 |
10 |
22 |
10 |
10 |
10 |
47 |
10 |
10 |
10 |
47 |
10 |
10 |
10 |
47 |
10 |
10 |
10 |
100 |
10 |
10 |
10 |
100 |
10 |
10 |
10 |
100 |
10 |
10 |
10 |
Table 1: Effect concentrations for time points < 96 h
Duration |
Endpoint |
Effect conc. |
Nom./Meas. |
Conc. based on |
Basis for effect |
Remarks |
3 h |
LC50 |
15mg/L |
nominal |
test mat. |
mortality |
95% CL = 14 - 16 mg/L |
6 h |
LC50 |
14mg/L |
nominal |
test mat. |
mortality |
95% CL = 13 - 15 mg/L |
24 h |
LC50 |
12 mg/L |
nominal |
test mat. |
mortality |
95% CL = 11 - 13 mg/L |
48 h |
LC50 |
11 mg/L |
nominal |
test mat. |
mortality |
95% CL = 9.9 - 12 mg/L |
72 h |
LC50 |
10 mg/L |
nominal |
test mat. |
mortality |
95% CL = 9.3 - 11 mg/L |
Table 2: Lethal time for the two highest concentrations
Concentration |
LT50 (h) |
95% CL (h) |
10 |
88 |
44 - 180 |
18 |
0.5 |
0.2 – 1.2 |
Table 3: Cumulative mortality data for brown shrimp exposed for 96 h
Concentration mg/L |
Cumulative mortality (initial population: 20) |
|||||
3 h |
6 h |
24 h |
48 h |
72 h |
96 h |
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
1.8 |
0 |
0 |
0 |
0 |
0 |
0 |
3.2 |
0 |
0 |
0 |
0 |
1 |
1 |
5.6 |
0 |
0 |
0 |
0 |
0 |
2 |
10.0 |
2 |
2 |
4 |
5 |
6 |
7 |
18.0 |
17 |
20 |
20 |
20 |
20 |
20 |
Description of key information
EC50 (48 h): 1.95 mg/L (TWA)
Key value for chemical safety assessment
Additional information
One key study investigating the acute toxicity of Alcohols, C9-11, branched and linear, ethoxylated to aquatic invertebrates is available. The GLP guideline study was conducted under semi-static conditions according to OECD 202. The test organism, Daphnia magna, was exposed to nominal test item concentrations of 0.313, 0.625, 1.25, 2.50 and 5.00 mg/L. Test solutions were renewed every 24 hours.
The test concentrations were analytically monitored by HPLC-MS/MS. The time weighted mean measured concentrations, based on three representative constituents of the UVCB substance, were 0.183, 0.366, 0.753, 1.54 and 3.62 mg/L The determined EC50 (48 h) was 1.95 mg/L (TWA).
Two supporting studies are available. In a static test on conducted with Daphnia magna an EC50 (48 h) of 2.5 mg/L (nominal) was determined. A semi-static test on the marine species Crangon crangon determined a LC50 (96 h) of 9.9 mg/L (nominal).
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