Pentyl propionate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to EPA-ASTM procedures and the report contains sufficient information to permit a meaningful evaluation of study results

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

other: EPA-ASTM guidelines

Deviations:

not specified

Principles of method if other than guideline:

not applicable

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Analytical monitoring:

no

Details on sampling:

no data

Vehicle:

no

Details on test solutions:

no data

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: fathead minnows
- Source: in-house
- Length at study initiation: size of the minnows ranged from 1.5-3.0 cm

ACCLIMATION
- Health during acclimation (any mortality observed): no information. Any chemical treatment required to control or prevent disease was stopped at least 5 days prior to testing

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

not applicable

Hardness:

40-60 mg/l as CaCO3

Test temperature:

no data available

pH:

no data available

Dissolved oxygen:

Minimal aeration was supplied when dissolved oxygen was below about 4 mg/l

Salinity:

not applicable

Nominal and measured concentrations:

no data

Details on test conditions:

The definitive test was conducted by EPA-ASTM procedures using 10 fathead minnows per test concentration in a total volume of 0.75 liters. Replicates were usually prepared. Temperature, fish survival, pH and dissolved oxygen levels were monitored during the test period of 96 hours. Minimal aeration was supplied when the dissolved oxygen was below about 4 mg/l.
Dechlorinated Charleston municipal water was used in tests as dilution water and in experimental controls and the same water was used for maintaining the fathead minnow population in-house. The incoming water was treated with activated carbon in two gallon filter yielding a chlorine-free dilution water with a pH very close to 7.0. Total hardness of the water measured 40-60 mg/l as CaCO3. The carbon beds were changed as needed to maintain water quality.

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

55.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 43.2-72.0 mg/l

Details on results:

No data

Results with reference substance (positive control):

not applicable

Reported statistics and error estimates:

no data

Sublethal observations / clinical signs:

None

Validity criteria fulfilled:

not specified

Conclusions:

Under the conditions of the study, the 96-hour LC50 of n-pentyl propionate to fathead minnows is 55.9 mg/l with 95% confidence limits ranging between 43.2 - 72.0 mg/l.

Executive summary:

The definitive test was conducted by EPA-ASTM procedures using 10 fathead minnows per test concentration in a total volume of 0.75 liters. Replicates were usually prepared. Temperature, fish survival, pH and dissolved oxygen levels were monitored during the test period of 96 hours. Minimal aeration was supplied when the dissolved oxygen was below about 4 mg/l. Dechlorinated Charleston municipal water was used in tests as dilution water and in experimental controls and the same water was used for maintaining the fathead minnow population in-house. The incoming water was treated with activated carbon in two gallon filter yielding a chlorine-free dilution water with a pH very close to 7.0. Total hardness of the water measured 40-60 mg/l as CaCO3. The carbon beds were changed as needed to maintain water quality.

Under the conditions of the study, the 96-hour LC50 of n-pentyl propionate to fathead minnows is 55.9 mg/l with 95% confidence limits ranging between 43.2 - 72.0 mg/l.

Description of key information

In a GLP study conducted following EPA-ASTM guidelines, the 96-hour LC50 of n-pentyl propionate was reported as 55.9 mg/l with 95% fiducial limits ranging between 43.2 - 72.0 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

55.9 mg/L

Additional information

In a GLP study conducted following EPA-ASTM guidelines and rated a Klimisch score of 2, the 96-hour LC50 of n-pentyl propionate to fathead minnows (Pimephales promelas) was reported as 55.9 mg/l with 95% fiducial limits ranging between 43.2 - 72.0 mg/l.