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EC number: 248-394-5 | CAS number: 27310-25-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted following OECD guideline, but report does not mention whether GLP was followed and sufficient data is available for interpretation of results.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Principles of method if other than guideline:
- OECD guideline 209 followed.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 7-aminonaphthalene-1,3,5-trisulphonic acid
- EC Number:
- 248-394-5
- EC Name:
- 7-aminonaphthalene-1,3,5-trisulphonic acid
- Cas Number:
- 27310-25-4
- Molecular formula:
- C10H9NO9S3
- IUPAC Name:
- 7-aminonaphthalene-1,3,5-trisulfonic acid
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): FAT 93527/A
- Physical state: Powder
- Analytical purity: ca. 50%
- Lot/batch No.: 0031026A0
- Expiration date of the lot/batch: January 10, 2006
- Storage condition of test material: room temperature
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
None
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: 1,10, and 100 mg test item/L
- Sampling method: To obtain the nominal test concentrations of: 1,10, and 100 mg test item/L the following volumes were taken from a stock solution of 0.2g/L: 0.5, 5, and 50 ml_ and filled up to 100 ml_ with tap water after adding of 3.2 mL synthetic sewage feed and 30 ml_ sludge.
- Sample storage conditions before analysis: The test item solutions were prepared close to the test start.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Water
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): To obtain the nominal test concentrations of: 1,10, and 100 mg test item/L the following volumes were taken from a stock solution of 0.2 g/L: 0.5, 5, and 50 ml_ and filled up to 100 ml_ with tap water after
adding of 3.2 mL synthetic sewage feed and 30 ml_ sludge.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: Not a laboratory culture. -Activated sludge of a communal sewage treatment plant (ARA Therwil) collected on April 09, 2001
- Preparation of inoculum for exposure: The inoculum was prepared 2 days before test begin.
- Pretreatment: The freshly collected activated sludge was washed 3 times with tap water. Subsequently the sludge was aerated. A sample of the activated
sludge was taken to determine the dry weight of the suspended solids.
- Initial biomass concentration: The final concentrations of the inoculum was about 4.0 g/L suspended solids.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- No data
- Test temperature:
- 23.2°C
- pH:
- 7.9 - 8
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- nominal test concentrations: 1,10, and 100 mg test item/L
- Details on test conditions:
- TEST SYSTEM
- Type (delete if not applicable): closed
- Aeration: 0.1 - 0.2 L/min. by Pasteurpipette
TEST MEDIUM / WATER PARAMETERS
Test Medium: A synthetic sewage feed was made by dissolving the following amounts of substances in 1 litre of deionized water:
peptone: 16.0 g
meat extract: 11.0g
urea: 3.0 g
NaCI: 0.7 g
CaCI2. 2H2O: 0.4 g
MgSO4 . 7H2O: 0.2 g
K2HPO4: 2.8 g - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: IC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The respiration rate of an activated sludge fed with a standard amount of synthetic sewage feed was measured after a contact time of 3 hours. The respiration rate of the same activated sludge in the presence of various concentrations of the test item under otherwise identical conditions was also measured. The inhibitory effect of test item at a particular concentrations was expressed as a percentage of the mean respiration rates of two controls. If possible IC50 and IC20 were calculated from inhibition values at different concentrations.
- Results with reference substance (positive control):
- None
- Reported statistics and error estimates:
- No statistics applied
Any other information on results incl. tables
Calculation of the IC50 and IC20 values:
The respiration rate of each test concentration was calculated as a percentage of the mean of the two control respiration rates. Since no 50% and 20% inhibition were observed, the IC50 and IC20 could not be calculated.
Validity of the test:
The test was considered valid because the respiration rates of the two controls were within 15% of each other.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- IC50 > 100 mg/L
IC20 > 100 mg/L - Executive summary:
The objective of this study was the estimation of toxicity of FAT 93527/A to activated sludge containing micro-organisms following OECD Guidelines for the Testing of Chemicals, Number 209. Activated sludge was exposed to different concentrations of the test item. The toxic effect was determined by measuring the respiration rate in relation to controls.
Activated sludge of a communal sewage treatment plant (ARA Therwil) collected on April 09, 2001 The inoculum was prepared 2 days before test begin. The freshly collected activated sludge was washed 3 times with tap water. Subsequently the sludge was aerated. A sample of the activated sludge was taken to determine the dry weight of the suspended solids.
The final concentrations of the inoculum was about 4.0 g/L suspended solids. To obtain the nominal test concentrations of: 1,10, and 100 mg test item/L the following volumes were taken from a stock solution of 0.2 g/L: 0.5, 5, and 50 ml and filled up to 100 ml with tap water after adding of 3.2 mL synthetic sewage feed and 30 ml sludge.
The respiration rate of an activated sludge fed with a standard amount of synthetic sewage feed was measured after a contact time of 3 hours. The respiration rate of the same activated sludge in the presence of various concentrations of the test item under otherwise identical conditions was also measured. The inhibitory effect of test item at a particular concentrations was expressed as a percentage of the mean respiration rates of two controls.
The respiration rate of each test concentration was calculated as a percentage of the mean of the two control respiration rates. Since no 50% and 20% inhibition were observed, the IC50and IC20could not be calculated.
Hence it was concluded that:
IC50 (3h) of the test item FAT 93527/A: > 100 mg/L
IC20(3h) of the test item FAT 93527/A: > 100 mg/L
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