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EC number: 700-567-0 | CAS number: 1231728-34-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Read-across from a well documented publication
Data source
Reference
- Reference Type:
- publication
- Title:
- Chemical Mutagenesis at the Thymidine Kinase Locus in L5178Y Mouse Lymphoma Cells: Results for 31 Coded Compounds in the National Toxicology Program
- Author:
- Myhr B C and Caspary W J
- Year:
- 1 991
- Bibliographic source:
- Environmental and Molecular Mutagenesis 18; 51-83 (1991)
Materials and methods
- Principles of method if other than guideline:
- Method: Clive et al. (1979), Mitchell et al. (1988), and Myhr and Caspary (1988)
- GLP compliance:
- not specified
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Tin dichloride
- EC Number:
- 231-868-0
- EC Name:
- Tin dichloride
- Cas Number:
- 7772-99-8
- Details on test material:
- Tin Dichloride [CAS No. 7772-99-8]; purity not reported, source: National Toxicology Program Chemical Repository.
Constituent 1
Method
- Target gene:
- thymidine kinase (TK) locus
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Details on mammalian cell type (if applicable):
- - Type and identity of media: RPM1 1640 or Fischer's medium
- Properly maintained: yes
- Periodically checked for Mycoplasma contamination: yes ervery 2-3 month
- Periodically checked for karyotype stability: yes/no
- Periodically "cleansed" against high spontaneous background: yes/no
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 homogenate was prepared from the livers of Aroclor 1254-induced male Fischer 344 rats.
- Test concentrations with justification for top dose:
- 0-80 ug/ml
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: not soluble in water
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Remarks:
- without S9
Migrated to IUCLID6: 5 nL/mL
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: MCA (3-methylcholanthrene, 2.5 ug/mL
- Remarks:
- with S9
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium(Fisher's treatment medium)
DURATION
- Exposure duration: incubation 4 hours
- Expression time (cells in growth medium): 48 h
- Selection time (if incubation with a selection agent): 48 h
SELECTION AGENT (mutation assays): trifluorothymidine (TFT)
NUMBER OF REPLICATIONS: two
NUMBER OF CELLS EVALUATED: 300000/plate seeded
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth, cloning efficiency - Evaluation criteria:
- Each experiment was evaluated for compliance with the quality control criteria before it was accepted for evaluation of the response. The upper limit for an acceptable CE was increased from 115% to 120%. The overall evaluation of the test chemical in the mouse lymphoma assay was based on the test condition yielding the greatest response.
- Statistics:
- Statistical analyses were performed using a computer program for both the MF trend and for comparisons between each dose level and the solvent control.
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- Stannous chloride was not mutagenic to L5178Y mouse lymphoma cells.
The lowest average RTG values obtained for treatments with soluble concentrations ranged from 30 -35 % without S9 to ca. 60 % with S9.
Precipitation was observed at 80 µg/mL in culture medium, and a slightly acidic pH shift in the medium was noted at 50 µg/mL. - Remarks on result:
- other: strain/cell type: mouse lymphoma L5178Y cells
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Tin dichloride is considered to be non-mutagenic, in the L5178Y mouse lymphoma cell mutation assay. - Executive summary:
In a mammalian gene mutation assay, mouse lymphoma cells (L5178 Y; thymidine kinase locus) cultured in vitro were exposed to tin dichloride up to a precipitating concentration of 80 µg/mL. The induction of small and large mutant colony populations was analyzed in the present and absence of mammalian metabolic activation.
Some erratic increases in MF of 1.5 to 1.8 fold were observed and these changes bore no relation to toxicity and were not repeatable among the three nonactivation assays and two S9 activation assays. The lowest average RTG values obtained for treatments with soluble concentrations ranged from 30 -35% without S9 to ca. 60% with S9. Precipitation was observed at 80 µg/mL in culture medium, and a slightly acidic pH shift in the medium was noted at 50 µg/mL.
The test chemical was dosed into medium from a solution in DMSO because it was not soluble in water, even at 100 µg/mL, which shifted the pH to 3.5. This observation suggested that the actual material tested was tin dichloride, which forms an insoluble basic salt in excess water. Since tin dichloride itself is soluble in water, possibly different toxic (and mutagenic) properties would have been obtained had the test chemical been more soluble and accessible to the cells. Also, the reduced toxicity in the presence of S9 mix suggested that tin dichloride is capable of interacting (in as yest unknown ways) with metabolic activation systems.
In conclusion, in the L5178Y mouse lymphoma cell mutation assay, tin dichloride is considered to be non-mutagenic.
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