Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 279-015-1 | CAS number: 78952-61-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 October 1989 to 18 January 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable. - Analytical monitoring:
- yes
- Details on sampling:
- Test concentrations: 0/560/ 1000 mg/L
- Vehicle:
- no
- Details on test solutions:
- The relevant amount of the test substance was weighed into a graduated flask (200 mL), filled up with test water and stirred for about 5 minutes with a magnetic stirrer. The test batches were present as red solutions. No segregation or formation of precipitates could be observed.
In order to determine the test substance concentration, water samples were taken at study end. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna (STRAUS)
Origin: Stock culture of Department of Toxicology (Hoechst AG)
Breeding conditions: beakers (height 235 mm, diameter 128 mm), filled with 2L test water (according ISO/DIS 7346/1) served as culture vessels. 20 adult daphnids were used per culture vessel. Animals were transferred to fresh prepared vessels once a week. The room temperature was at 20 ± 1 °C. The breeding room was illuminated by diffuse light in a day/night cycle of 12 hours. The animals were fed with monocellular green algae (Scenedemus subspicatus) and a fine suspension of Tetra min dry food (Tetra Werke, Melle).
To collect test animals with an age of at least 6 hours and a maximum of 24 hours, approx. 3 weeks old parent animals were separated in a cristallising dish (3L volume) filled with test medium for 18 hours. After 18 hours the adults were separted from the young daphnids by sieving through sieves of different mesh size. Adults remain on the upper crude sieve while the young daphnids are found on the fine sieve. These animals were used for the study after further 6 hours. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No post exposure observation period
- Hardness:
- No data
- Test temperature:
- 20.4 - 21.3°C
- pH:
- 7.7 - 8.3
- Dissolved oxygen:
- 8.6 - 9.5 mg/L
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- 0, 560 and 1000 mg/L.
- Details on test conditions:
- The study was conducted in 2 stages. 20 daphnids, divided into two parallel groups of 10 animals, were used for each concentration.
Water for dilution
Reconstituted water, composition according to ISO/DIS 7346/1 was used as water for dilution.
Preparation was carried out in a unit consisting of two Hostalen®- lined steel vessels with a capacity of 1700 litres each.
The dilution water was prepared as described and was aerated until oxygen saturation. The pH was measured before the use of each study batch. It was at 8.3.
Test conditions
The study was conducted in a static system. As test chambers 300 mL cristallising dishes were used (height 55 mm, diameter 95 mm, water level about 32 mm), each filled with 200 mL test water. The room temperature was about 21 °C. The room was illuminated by fluorescent tubes for 12 hours each day.
Test procedure
After the test concentrations had been prepared and water parameters were recorded, 10 daphnids were used in each test chamber. The daphnids were not fed during the entire study period. Inspection of the daphnids took place after 24 and 48 hours and involved recording the immobility and further possible deviations to the control group. Immobile daphnids were removed from the chambers. Those animals which were not able to swim within 15 seconds after gentle agitation of the test container were considered to be immobile. The water parameters were measured after adding the substance and at the end of the test. - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Probit analysis could not be carried out due to the lethality rate. As the factor for the concentration steps was 1.8 no further concentrations were tested. The EC50 range was given.
Under the conditions of this test with Reaktiv-Rot F-66 813 FW the following immobility rates were observed after 24 and 48 hours:
Concentration (mg/l) 24 hours 48 hours
Absolute % Absolute %
0 0/40 0 0/40 0
560 0/20 0 0/20 0
1000 0/20 0 2/20 10
EC0 (mg/L)
highest tested conc. With no immobility 1000 560
EC50 (mg/L) > 1000 > 1000 - Results with reference substance (positive control):
- Not applicable.
- Reported statistics and error estimates:
- No data
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 > 1000 mg/L
- Executive summary:
The study on acute toxicity to daphnids with the Test Substance meets the requirements of EEC Directive 79/831 Annex V Methods for the determination of ecotoxicity 5.1.2 Acute toxicity for daphnia and the OECD Guidelines for Testing of Chemicals, Section 2 - Effects on biotic systems 202 Daphnia sp., 14-day reproduction test (including an acute immobilisation test). Study conducted in compliance with GLP.
Testing for acute toxicity in Daphnia magna (water flea) over a total of 48 hours yielded the following immobility values:
after 24 hours (mg/L) after 48 hours (mg/L)
EC0 1000 560
EC50 > 1000 > 1000
The concentrations tested were 0 / 560 / 1000 mg/L. Immobility was observed at 1000 mg/L.
The test batches were present as red solutions. No segregation or formation of precipitates could be observed.
In order to determine the substance concentration, water samples were taken at study end. All mean values were above 80% of the nominal concentration.
Reference
Immobility – individual values
Concentration (mg/L) |
Test-chamber No. |
Study-start (date) |
Immobility hours after study start |
|
24 |
48 |
|||
0 |
80a |
10-Jan-90 |
0/10 |
0/10 |
0 |
80b |
10-Jan-90 |
0/10 |
0/10 |
1000 |
36a |
10-Jan-90 |
0/10 |
0/10 |
1000 |
36b |
10-Jan-90 |
0/10 |
2/10 |
0 |
90a |
16-Jan-90 |
0/10 |
0/10 |
0 |
90b |
16-Jan-90 |
0/10 |
0/10 |
560 |
1a |
16-Jan-90 |
0/10 |
0/10 |
560 |
1b |
16-Jan-90 |
0/10 |
0/10 |
Description of key information
EC50 (48h): > 1000 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Remarks:
- (48 h)
- Effect concentration:
- > 1 000 mg/L
Additional information
The study on acute toxicity to daphnids with the structural analogue meets the requirements of EEC Directive 79/831 Annex V Methods for the determination of ecotoxicity 5.1.2 Acute toxicity for daphnia and the OECD Guidelines for Testing of Chemicals, Section 2 - Effects on biotic systems 202 Daphnia sp., 14-day reproduction test (including an acute immobilisation test). Study conducted in compliance with GLP.
Testing for acute toxicity in Daphnia magna (water flea) over a total of 48 hours yielded the following immobility values:
|
after 24 hours (mg/L) |
after 48 hours (mg/L) |
EC0 |
≥ 1000 |
560 |
EC50 |
> 1000 |
> 1000 |
The concentrations tested were 0, 560, and 1000 mg/L. Immobility was observed at 1000 mg/L.
The test batches were present as red solutions. No segregation or formation of precipitates could be observed.
In order to determine the substance concentration, water samples were taken at study end. All mean values were above 80% of the nominal concentration.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)