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EC number: 211-687-3 | CAS number: 686-31-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not required
- Details on sampling:
- A sample of each mixture is poured into a BOD bottle (following the order of preparation). An oxygen electrode is inserted into the bottle. The oxygen concentration is measured every minute during a period of 10 min.
- Vehicle:
- no
- Details on test solutions:
- N-Allylthiourea solution
Following the guideline, a 2.32 g/L solution of N-allylthiourea (ATU) was prepared. Addition of 2.5 mL of this solution into a final volume of 500 mL gives a final concentration of 11.6 mg/L (0.1 mM) of ATU known to be sufficient to cause 100 % inhibition of 1.5 g/L of suspended solids.
Introduction of the test item
The test item was directly introduced into the flasks, as its low solubility did not allow preparation of a stock solution.
Nitrification inhibitor (ATU):
Chemical name: N-Allylthiourea
CAS number: 109-57-9
EC number: 203-683-5
Date of reception in the test facility: 03/01/11
Expiration date of the test item: 03/01/11
Batch number/supplier: 10J130009 / VWR
Characteristics: white to light yellow crystals
Empirical formula: C4H8N2S
Purity: 99 %
Molecular weight: 116.18 g/mol. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The inoculum comes from the activated sludge of the biological domestic wastewater treatment plant from Abidos. The effluent, obtained one days before seeding the flasks, is centrifuged at approximately 20 °C for 10 min at 1000 g; the base is re-suspended in ultra-pure water in order to keep the concentration unchanged with respect to the sample. The amount of dry material in the inoculum, is determined by dessiccation at 105 °C until a constant weight. From this result the amount of wet sludge can be calculated in order to obtain an activated sludge with a mixed liquor suspended solids level of 4 g/L (+/- 10%). The inoculum is preconditioned (aeration). Every day, synthetic sewage feed are added until the day the flasks are inoculated.
Value of suspended solids was 5.11 g/L. Then a dilution was performed to obtain 3 g/L. This level corresponds to a concentration of 1.5 g/L in the final test medium. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- incubated at 20 +/- 2 °C
- Dissolved oxygen:
- The dissolved oxygen concentrations are determined electrochemically using an oxygen electrode (WTW Stirrox G) and meter (WTW OXI 538).
- Nominal and measured concentrations:
- The test item was directly introduced into the flasks in order to obtain concentrations from 50 to 1000 mg/L (50, 105.7, 223.6, 472.9 and 1000 mg/L) with a factor of 2.11.
- Details on test conditions:
- The tests are carried out in 500 mL flasks, with the following repartition:
- 16 ml of synthetic sewage feed
- adequate volume of test item and ultra-pure water for different concentrations
- 250 ml of sludge suspension
At T0, the first control (control1) without item is prepared. Then every 15 min. the others vessels with different concentrations of item are prepared in turn. Finally, the second control (control 2) is prepared.
All suspensions are aerated and stirred during 3 hours and incubated at 20 +/- 2 °C without light. Then a sample of each mixture is poured into a BOD bottle (following the order of preparation). An oxygen electrode is inserted into the bottle. The oxygen concentration is measured every minute during a period of 10 min. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 480 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Details on results:
- See tables below.
EC10 were also determined, however calculations are considered not reliable. - Results with reference substance (positive control):
- See tables below
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 for the test item was determined to be higher than the highest tested concentration: EC50 > 1000 mg/L
- Executive summary:
The purpose of this study was to determine the effect of the test item on bacteria according to the OECD guideline 209 “Activated Sludge, Respiration Inhibition Test”. The effect is assessed by measuring the micro-organisms respiration rates on a sample of activated sludge during an incubation period of 3 hours. Three respiration rates were evaluated : total respiration rate, heterotrophic respiration rate (in presence of N-Allylthiourea) and nitrification respiration rate by difference between total and heterotrophic respiration rates.
EC50 of the reference item 3,5-dichlorophenol was 9.8 mg/L, between 2 and 25 mg/L for total respiration;15 mg/L, between 5 and 40 mg/l for heterotrophic respiration, but could not be determined for nitrification respiration.
EC50 for the test item was determined to be higher than the highest tested concentration: EC50 > 1000 mg/L
Reference
TERT-AMYL PEROXY-2-ETHYLHEXANOATE INHIBITION RATE
Nominal concentrations (mg/L) |
T0 |
T = 180 (min.) |
Taux de respiration (O2 mg/L/h) |
% inhibition |
Control 1 |
7,4 |
1,1 |
47,3 |
- |
Control 2 |
7,5 |
1,4 |
45,8 |
- |
50 |
7,5 |
1,3 |
41,3 |
11,1 |
105,7 |
7,5 |
1,5 |
40,0 |
14,0 |
223,6 |
7,3 |
1,2 |
40,7 |
12,5 |
472,8 |
7,3 |
1,4 |
39,3 |
15,4 |
1000 |
7,6 |
2,1 |
36,7 |
21,1 |
TERT-AMYL PEROXY-2-ETHYLHEXANOATE + ATU INHIBITION RATE Oxygen measurements (mg O2 /L) at 3 hours
Nominal concentrations (mg/L) |
T0 |
T = 180 (min.) |
Taux de respiration (O2 mg/L/h) |
% inhibition |
Control 1 |
7,8 |
2,3 |
36,7 |
- |
Control 2 |
7,5 |
1,7 |
38,7 |
- |
50 |
7,6 |
2,2 |
36,0 |
4,4 |
107,5 |
7,6 |
2,2 |
36,0 |
4,4 |
223,6 |
8,0 |
3,1 |
32,7 |
13,3 |
472,8 |
7,4 |
2,0 |
36,0 |
4,4 |
1000 |
7,8 |
2,8 |
33,3 |
11,5 |
Respiration rates recapitulation and EC50 for3 ,5-dichlorophenol
|
Total Respiration without ATU |
Heterotrophic Respiration with ATU |
Nitrification Respiration by difference |
|||
Control |
TR mg/L.h |
CV % |
HR mg/L.h |
CV % |
NR mg/L.h |
CV % |
C1 |
46,3 |
|
60,0 |
|
-13,7 |
|
C2 |
47,3 |
1,5 |
60,9 |
1,1 |
-13,6 |
- |
Mean |
46,8 |
|
60,5 |
|
-13,7 |
|
DCP (mg/L) |
TR mg/L.h |
% Inh. |
HR mg/L.h |
% Inh. |
NR mg/L.h |
% Inh. |
0,098 |
44,0 |
6,0 |
59,1 |
2,2 |
-15,10 |
|
0,439 |
44,0 |
6,0 |
59,1 |
2,2 |
-15,10 |
|
1,975 |
38,4 |
17,9 |
59,1 |
2,2 |
-20,70 |
- |
8,889 |
31,2 |
33,3 |
42,0 |
30,5 |
-10,80 |
|
40 |
7,2 |
84,6 |
9,3 |
84,6 |
-2,10 |
|
|
Total Respiration |
Heterotrophic Respiration |
Nitrification Respiration |
|||
EC50 (mg/L) |
9,80 |
15,0 |
ND |
Respiration rates recapitulation, EC50 for TERT-AMYL PEROXY-2-ETHYLHEXANOATE
|
Total Respiration without ATU |
Heterotrophic Respiration with ATU |
Nitrification Respiration by difference |
|||
Control |
TR mg/L.h |
CV % |
HR mg/L.h |
CV % |
NR mg/L.h |
CV % |
C1 |
47,3 |
|
36.7 |
|
10.6 |
|
C2 |
45,8 |
2.3 |
38.7 |
3.8 |
7.1 |
28.0 |
Mean |
46,6 |
|
37.7 |
|
8.8 |
|
Luperox 575 (mg/L) |
TR mg/L.h |
% Inh. |
HR mg/L.h |
% Inh. |
NR mg/L.h |
% Inh. |
50 |
41,3 |
11,3 |
36,0 |
4,5 |
5,30 |
0,00 |
105,7 |
40,0 |
14,1 |
36,0 |
4,5 |
4,00 |
54,80 |
223,6 |
40,7 |
12,6 |
32,7 |
13,3 |
8,00 |
9,60 |
472,8 |
39,3 |
15,6 |
36,0 |
4,5 |
3,30 |
62,71 |
1000 |
36,7 |
21,2 |
33,3 |
11,7 |
3,40 |
61,58 |
|
Total Respiration |
Heterotrophic Respiration |
Nitrification Respiration |
|||
EC50 (mg/L) |
> 1000 |
> 1000 |
480 |
Description of key information
OECD 209, GLP, key study, validity 1:
EC50> 1000 mg/L
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
The toxicity of TERT-AMYL PEROXY-2-ETHYLHEXANOATE to microorganism was estimated in a study performed according to OECD 209 guideline and GLP requirements. EC50 for the test item was determined to be higher than the highest tested concentration: EC50 > 1000 mg/L
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