5-(methoxymethyl)-2-furaldehyde

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 6, 2011 - August 4, 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: This study has been performed in accordance with OECD 301B (1992) and according to GLP principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Version / remarks:

(1992)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Version / remarks:

(2008)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: ISO Standard 9439 “Water Quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - carbon dioxide evolution test (1999)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

5-(methoxymethyl)furan-2-carbaldehyde

EC Number:

700-511-5

Cas Number:

1917-64-2

Molecular formula:

C7H8O3

IUPAC Name:

5-(methoxymethyl)furan-2-carbaldehyde

Details on test material:

- Name of test material (as cited in study report): MMF
- Description: Clear yellow liquid
- CAS Number: 1917-64-2

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source: The source of test organisms was activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Treatment: The freshly obtained sludge was kept under continuous aeration until further treatment. The concentration of suspended solids was 4.2 g/l in the concentrated sludge (information obtained from the municipal sewage treatment plant). Before use, the sludge was allowed to settle (60 minutes) and the liquid was decanted for use as inoculum at the amount of 10 ml/l of mineral medium.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

20 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance

Reference substance:

acetic acid, sodium salt

Remarks:

(40 mg/L)

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

1

Sampling time:

2 d

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

4

Sampling time:

9 d

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

4

Sampling time:

14 d

% Degradation 4

Parameter:

% degradation (CO2 evolution)

Value:

6.5

Sampling time:

19 d

% Degradation 5

Parameter:

% degradation (CO2 evolution)

Value:

10

Sampling time:

29 d

Details on results:

- In the toxicity control more than 25% biodegradation occurred within 14 days (38%, based on ThCO2). Therefore, the test substance was assumed not to inhibit microbial activity.

BOD5 / COD results

Results with reference substance:

The positive control substance was biodegraded by 83% within 14 days.

Any other information on results incl. tables

Theoretical CO2 production

- The ThCO2 of MMF was calculated to be 2.20 mg CO2/mg.

- The ThCO2 of sodium acetate was calculated to be 1.07 mg CO2/mg.

pH values 

Test medium:	At the start of the test:	On day 28:
Blank control (A)	7.6	7.6
Blank control (B)	7.6	7.6
Positive control	7.6	7.9
MMF (A)	7.6	7.6
MMF (B)	7.6	7.6
Toxicity control	7.6	7.9

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable under the conditions of the modified Sturm test

Conclusions:

In accordance with OECD 301B (1992) and according to GLP principles the ready biodegradability of MMF was investigated. It was determined that MMF is not readily biodegradable under the conditions of the modified Sturm test.

Executive summary:

In this study the ready biodegradability of MMF was investigated. The study procedures were based on OECD 301B. The test substance was tested in duplicate at 20 mg/l, corresponding to 12 mg TOC/l. Test duration was 28 days (last CO2-measurement on the 29th day). The relative biodegradation values calculated from the measurements performed during the test period revealed 10% biodegradation of MMF in both test bottles. In the toxicity control, MMF was found not to inhibit microbial activity. All the validity criteria were fulfilled. It was determined that MMF is not readily biodegradable under the conditions of the modified Sturm test.