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EC number: 223-289-7 | CAS number: 3811-04-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No GLP and no standard test protocol, but test described in enough detail.
- Guideline:
- other: Standard 72h growth inhibition bioassay (Stauber etal., 1994)
- Principles of method if other than guideline:
- Toxicity of chlorate at 3 nitrate concentrations (<0.005, 1 and 15 mg/l NO3) was studies using a standard 72 hours growth inhibtion bioassay (Stauber et al., 1994). At a nitrate concentration of <0.005 mg/l the toxicity of chlorate at 5 concentrations (0.5, 1, 5, 10 and 20 mg/l) was determined. At higher nitrate concentration (1 and 15 mg/l), 5 concentrations of chlorate were tested ranging from 1-1000 mg/l depending on the alga and nitrate concentration in each experiment.
Cell densities were determined daily using a Coulter Multisizer IIE with 70 µm aperture. - GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- See details in analytical methods.
- Vehicle:
- no
- Details on test solutions:
- Three nitrate levels were used: <0.005, 1 and 15 mg/l NO3. At nitrate concentrations of <0.005 mg/l the toxicity of chlorate was tested at 0.5, 1, 5, 10 and 20 mg/l ClO3-. At nitrate concentrations of 1 and 15 mg/l the test concentrations were ranging from 1 to 1000 mg/l ClO3-, depending on the alga and nitrate concentration in each experiment.
- Test organisms (species):
- other: other algae: Nitzschia closterium
- Details on test organisms:
- Nitzschia closterium (Ehrenberg) W. Smith, originally isolated from Port Hacking, NSW, Strain CS-5c.
Culture medium: Medium f (Guillard and Ryther, 1962) with the iron and trace element concentrations halved. - Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Nominal and measured concentrations:
- At nitrate concentrations of <0.005 mg/l the toxicity of chlorate was tested at 0.5, 1, 5, 10 and 20 mg/l ClO3-.
At nitrate concentrations of 1 and 15 mg/l the test concentrations were ranging from 1 to 1000 mg/l ClO3-, depending on the alga and nitrate concentration in each experiment.
Chlorate was measured in each bioassay flask at the beginning and end of the test and was always within 10% of the nominal chlorate concentration. - Details on test conditions:
- Seawater for the preparation of the test medium was collected 0-1 km offshore from Port Hacking, NSW. The seawater was filtered through a 0.45 µm membrane filter and stored at 4 °C.
- Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: chlorate ion
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 1.6 - 2.3 mg/l Nitrate conc: < 0.005 mg/l
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: chlorate ion
- Basis for effect:
- growth rate
- Remarks on result:
- other: Nitrate conc: 1 mg/l
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: chlorate ion
- Basis for effect:
- growth rate
- Remarks on result:
- other: Nitrate conc: 15 mg/l
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: chlorate ion
- Basis for effect:
- growth rate
- Remarks on result:
- other: Nitrate conc: 15 mg/l
- Details on results:
- - Analytical results: Chlorate was measured in each bioassay flask at the beginning and end of the test and was always within 10% of the nominal chlorate concentration. Chlorate concentrations remained stable over the 3 days and chlorate was not reduced to chlorite or chloride in the light either in the presence or absence of algal cells.
- Reported statistics and error estimates:
- Data analyses were performed using Toxcalc version 5.0 (Tidepool scientific software).
- Validity criteria fulfilled:
- not specified
- Conclusions:
- EC50:
- 1 mg nitrate/l: 10 mg ClO3-/l corresponds to 15 mg KClO3/l
- 15 mg nitrate/l: >500 mg ClO3-/l corresponds to >734 mg KClO3/l
NOEC:- 15 mg nitrate/l: 100 mg ClO3-/l corresponds to 147 mg KClO3/l
The results obtained at a nitrate concentration of <0.005 mg/l were not considered to be valid due to a low doubling rate in the control vessels and can not be used, therefore. - Executive summary:
Toxicity of chlorate at 3 nitrate concentrations (<0.005, 1 and 15 mg/l NO3) was studies using a standard 72 hours growth inhibtion bioassay (Stauber et al., 1994). At a nitrate concentration of <0.005 mg/l the toxicity of chlorate at 5 concentrations (0.5, 1, 5, 10 and 20 mg/l) was determined. At higher nitrate concentration (1 and 15 mg/l), 5 concentrations of chlorate were tested ranging from 1-1000 mg/l depending on the alga and nitrate concentration in each experiment.
Cell densities were determined daily using a Coulter Multisizer IIE with 70 µm aperture.
Nitzschia closterium was used as test organism.
EC50:
- <0.005 mg nitrate/l: 1.9 mg ClO3-/l (95% c.i. 1.6-2.3 mg/l) corresponds to 2.8 mg KClO3/l (95% c.i. 2.3-3.4 mg/l)
- 1 mg nitrate/l: 10 mg ClO3-/l corresponds to 15 mg KClO3/l
- 15 mg nitrate/l: >500 mg ClO3-/l corresponds to >734 mg KClO3/l
NOEC:- 15 mg nitrate/l: 100 mg ClO3-/l corresponds to 147 mg KClO3/l
The results obtained at a nitrate concentration of <0.005 mg/l were not considered to be valid due to a low doubling rate in the control vessels and can not be used, therefore.
Reference
Recalculation of the results from mg/l ClO3- to KClO3. EC50:The biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period. This corresponds to a specific growth rate of 0.92 day-1. For the test performed at <0.005 mg nitrate/l this was not the case and therefore this test result is not valid and can not be used.
- <0.005 mg nitrate/l: 1.9 mg ClO3-/l (95% c.i. 1.6-2.3 mg/l) corresponds to 2.8 mg KClO3/l (95% c.i. 2.3-3.4 mg/l)
- 1 mg nitrate/l: 10 mg ClO3-/l corresponds to 15 mg KClO3/l - 15 mg nitrate/l: >500 mg ClO3-/l corresponds to >735 mg KClO3/l NOEC:- 15 mg nitrate/l: 100 mg ClO3-/l corresponds to 147 mg KClO3/l Control cell division rate (doublings/day) - <0.005 mg nitrate/l: 0.8 - 1 mg nitrate/l: 1.1 - 15 mg nitrate/l: 1.3 Remark from reviewer: According to OECD guideline 201 Freshwater Alga and Cyanobacteria, Growth Inhibition Test the
Description of key information
The lowest endpoint for freshwater algae and plants is determined for Lemna minor which is described in section 6.1.6.
The lowest endpoint for marine algae with a more standard nitrate concentration was found for Nitzschia closterium, with a NOEC of 100 mg ClO3-/l, which is equal to 147 mg KClO3/l.
Key value for chemical safety assessment
- EC50 for marine water algae:
- 735 mg/L
- EC10 or NOEC for marine water algae:
- 147 mg/L
Additional information
No studies with freshwater aquatic algae are available on potassium chlorate. The key study for the endpoint aquatic algae and plants is a study with Lemna minor described in section 6.1.6.
Two tests (valid with restriction) were found for marine algae performed with potassium chlorate (Stauber, 1998). The endpoints were expressed in mg chlorate/l. The endpoints were recalculated to mg potassium chlorate/l. Stauber performed the test at three different nitrate levels namely <0.005, 1 and 15 mg nitrate/l. At the lower nitrate concentrations cell growth in the controls was not according to the standard criteria and these results cannot be used therefore.
The original data for the EC50was for Dunaliella tertiolecta > 1000 mg chlorate/l (15 mg/l nitrate) and for Nitzschia closterium 10 mg chlorate/l and > 500 mg chlorate/l, 1 and 15 mg/l nitrate respectively. The NOEC values were 500 mg chlorate/l for Dunaliella tertiolecta and 100 mg chlorate/l for Nitzschia closterium.
The lowest short-term value for marine algae species was found for Nitzschia closterium, the EC50was 15 mg/l at 1 mg nitrate/l.
The difference in toxicity noted between marine and freshwater algae, appears to be related more to the relative difference in concentration of nitrate in freshwater and marine compartments than to different mechanisms of toxicity between species. The concentration of nitrate in the test water influences the effect concentration of chlorate (Stauber, 1998) indicating that competitive inhibition occurs between nitrate and chlorate with excess nitrate inhibiting chlorate toxicity. This is supported by the acute studies on marine species using chlorate performed by Stauber at several nitrate concentrations.
When comparing studies, comparison should be made of studies performed under the same standard conditions as much as possible. A more standard nitrate concentration is 15 mg/l, therefore this test result should be used. For Nitzschia closterium a NOEC of 100 mg ClO3-/l, which is 147 mg/ potassium chlorate was determined under more standard conditions.
One valid test with marine algae is available (Tobiesen, 2010a) for sodium chlorate. Tobiesen performed a standard test with Skeletonema costatum according to guideline and GLP. Chemical analyses were performed on the test concentrations. He found that S. Costatum was not sensitive to sodium chlorate, with a NOEC greater than or equal to 1000 mg/l. On a molecular weight basis this would be 1151 mg/l potassium chlorate.
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