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EC number: 473-160-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2007-09-14 to 2007-12-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 B (Bioaccumulation: Semi-static Fish Test)
- Version / remarks:
- 1996
- Deviations:
- no
- GLP compliance:
- yes
- Details on sampling:
- - Samples method: Samples from both test solutions and test organisms were taken daily from the 26th day to the last day during the exposure test period. The samples from test organisms were treated by extracting their lipid component that was collected from the test fish, and the extracted solutions of the test substance were obtained. The concentrations of the test substance in exposure solutions and extracted solutions were measured.
- Vehicle:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: Amazonas Commercial Aquarium, Shanghai
- Mean body length: 2.54 ± 0.11 cm
- Mean body weight: 0.25 ± 0.06 g
- Feeding during test: No
ACCLIMATION
- Acclimation period: One week
- Acclimation conditions: Prior to the test start, the fish were acclimated under conditions of water quality and illumination similar to those used in the test.
- Type and amount of food: During holding and acclimatization until one day before the start of the test the fish were fed with a commercial fish diet.
- Health during acclimation: No mortality was observed during the acclimatation period. During holding and acclimatization no fish died in the test fish batch and all fish were healthy. - Route of exposure:
- aqueous
- Test type:
- semi-static
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 28 d
- Hardness:
- 10 - 250 mg CaCO3 per liter
- Test temperature:
- 23 ± 1 °C
- pH:
- The pH values in the treatments and the controls ranged from 7.44 to 8.05
- Dissolved oxygen:
- the dissolved oxygen concentration was always 80 % or higher
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass vessel
- Type: open
- Volume: 15 L
- Renewal rate of test solution (frequency/flow rate): 2 days
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control / vehicle control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated drinking water is used in the test.
- Holding medium different from test medium: no
- Intervals of water quality measurement: The dissolved oxygen concentration, the pH and temperature in the controls and the test
solutions should be measured once every two days during the test.
- Intervals of test medium replacement: 2 days
OTHER TEST CONDITIONS No
- Adjustment of pH: No
- Photoperiod: light-dark cycle was 12h: 12h.
RANGE-FINDING / PRELIMINARY STUDY
Not performed - Nominal and measured concentrations:
- - Nominal concentration: 0 (control), 5 and 50 mg/L
- Measured concentration (mean): 6.2 and 59.1 mg/L from the first day to the third dayof sampling. - Reference substance (positive control):
- yes
- Key result
- Conc. / dose:
- 5 mg/L
- Type:
- BCF
- Value:
- < 0.05 dimensionless
- Key result
- Conc. / dose:
- 50 mg/L
- Type:
- BCF
- Value:
- < 0.46 dimensionless
- Results with reference substance (positive control):
- The sensitivity of the Brachydanio rerio and conformity with the procedure were supported by the result of the reference substance, δ-666 standard solution. The BCF of δ-666 standard solution was calculated as 2.1 x 10E4 which fulfilled the criteria for a valid test that the BCF of δ-666 standard solution should range in 1.5 x 10E4~3.0 x 10E4.
- Details on results:
- In the controls and the treatments at the treatment of 50 and 5 mg/L (mean measured concentration 59.1 and 6.2 mg/L) no fish death was determined during the limit test period of 28 days. Based on the measured concentrations in the exposure test medium and the test organism, the bioconcentration factor (BCF) of the test substance to zebra fish was calculated as < 0.05 and < 0.46, respectively at the treatment of 50 and 5 mg/L (mean measured concentration 59.1 and 6.2 mg/L). Therefore, the bioconcentration factor (BCF) of the test substance to zebra fish was < 100. In conclusion, the test substance had no obvious bioconcentration effects on zebra fish under the present conditions of the test.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The bioconcentration factor (BCF) of the test substance Blue MGi 1037-Na to zebra fish was <100.
- Executive summary:
The bioconcentration effect of the test substance Blue MGi 1037-Na to zebra fish (Brachydanio rerio) was determined in a 28-day semi-static test with a test medium renewal every two days. An exposure test at concentrations of 50 mg/L and 5 mg/L was performed in accordance with the result of the fish acute toxicity test to check out the bioconcentration effect of test substance on the test organisms. Thus, the treatments at concentrations of 50 mg/L and 5 mg/L and the controls were tested. Samples from both test solutions and test organisms were taken daily from the 26th day to the last day during the exposure test period. The samples from test organisms were treated by extracting their lipid component that was collected from the test fish, and the extracted solutions of the test substance were obtained. The concentrations of the test substance in exposure solutions and extracted solutions were measured.
The analytically measured test substance concentrations in the exposure test medium were 59.0 mg/L and 6.1 mg/L, 59.0 mg/L and 6.2 mg/L, 59.2 mg/L and 6.2 mg/L, respectively from the first day to the third day during the sampling period. All of the analytically measured test substance concentrations in the extracted solution were <0.05 mg/L from the first day to the third day of sampling. In the controls and the treatments at the treatment of 50mg/L and 5mg/L (mean measured concentration 59.1 mg/L and 6.2mg/L) no fish death was determined during the limit test period of 28 days. Based on the measured concentrations in the exposure test medium and the test organism, the bioconcentration factor (BCF) of the test substance to zebra fish was calculated as <0.05 and <0.46, respectively at the treatment of 50 mg/L and 5 mg/L (mean measured concentration 59.1mg/L and 6.2mg/L). Therefore, the bioconcentration factor (BCF) of the test substance Blue MGi 1037-Na to zebra fish was <100. In conclusion, the test substance Blue MGi 1037-Na had no obvious bioconcentration effects on zebra fish under the present conditions of the test.
Reference
Description of key information
The bioconcentration factor (BCF) of the test substance Blue MGi 1037-Na to zebra fish was determined to be < 100.
Key value for chemical safety assessment
Additional information
The bioconcentration effect of the test substance Blue MGi 1037-Na to zebra fish (Brachydanio rerio) was determined in a 28-day semi-static test with a test medium renewal every two days. An exposure test at concentrations of 50 and 5 mg/L was performed in accordance with the result of the fish acute toxicity test to check out the bioconcentration effect of test substance on the test organisms. Thus, the treatments at concentrations of 50 and 5 mg/L and the controls were tested.
Samples from both test solutions and test organisms were taken daily from the 26th day to the last day during the exposure test period. The samples from test organisms were treated by extracting their lipid component that was collected from the test fish, and the extracted solutions of the test substance were obtained. The concentrations of the test substance in exposure solutions and extracted solutions were measured.
The analytically measured test substance concentrations in the exposure test medium were 59.0 and 6.1 mg/L, 59.0 and 6.2 mg/L, 59.2 and 6.2 mg/L, respectively from the first day to the third day during the sampling period. All of the analytically measured test substance concentrations in the extracted solution were < 0.05 mg/L from the first day to the third day of sampling.
In the controls and the treatments at the treatment of 50 and 5 mg/L (mean measured concentration 59.1 and 6.2 mg/L) no fish death was determined during the limit test period of 28 days. Based on the measured concentrations in the exposure test medium and the test organism, the bioconcentration factor (BCF) of the test substance to zebra fish was calculated as < 0.05 and < 0.46, respectively at the treatment of 50 and 5 mg/L (mean measured concentration 59.1 and 6.2 mg/L). Therefore, the bioconcentration factor (BCF) of the test substance Blue MGi 1037-Na to zebra fish was determined to be <100.
In conclusion, the test substance Blue MGi 1037-Na had no obvious bioconcentration effects on zebra fish under the present conditions of the test. Based on these results and the bioconcentration toxicity grading criteria in the Guidelines for the Hazard Evaluation of New Chemical Substances (HJ/T154-2004), the hazardous level of the test substance is medium.
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