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EC number: 213-107-4 | CAS number: 924-50-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 798.5265 (The Salmonella typhimurium Bacterial Reverse Mutation Test)
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Directive 92/69, L 383 A, Annex B14
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3-Methylcrotonsäuremethylester
- IUPAC Name:
- 3-Methylcrotonsäuremethylester
- Reference substance name:
- Methyl 3-methyl-2-butenoate
- EC Number:
- 213-107-4
- EC Name:
- Methyl 3-methyl-2-butenoate
- Cas Number:
- 924-50-5
- Molecular formula:
- C6H10O2
- IUPAC Name:
- methyl 3-methylbut-2-enoate
- Test material form:
- other: Clear colourless to yellowish liquid
- Details on test material:
- Purity: 98.7 %, Storage: Darkness at approximately 20°C in a fume cupboard
Constituent 1
Constituent 2
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- Formulation of the test compounds: Dissolved in DMSO at appropriate concentrations immediately before use.
4,20,100,500,2500, 5000 µg/plate with and without metabolic acivation system. control groups: with and without metabolic activation, - Vehicle / solvent:
- DMSO to dissolve the test substance.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- other: 2- Aminoanthracene
- Details on test system and experimental conditions:
- Sterility of S9-mix and the test compound were indicated by the absence of contamination on the test material and S9-mix sterility check plates.
Experimental conditions in vitro: 37°C in the incubator. - Evaluation criteria:
- A test compound is evaluated as mutagenic if it has:
a) A 2 fold increase in number of the revertants per plate of at least one of the test strains over the mean number of revertants per plate of the appropriate vehicle control at complete bacterial background lawn.
b) A test compound induces a dose-relevant increase in the mean number of revertants per plate of at least one of the tester strains over the mean number of revertants per plate of appropriate vehicle control in at least two or three concentrations of the test compound at complete bacterial lawns.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Additional information on results:
- See attachment (Attached background material)
Applicant's summary and conclusion
- Conclusions:
- The test item is not mutagenic in these bacterial test systems either with or without exogenous metabolic activation.
- Executive summary:
Dimethylacrylsäuremethylester was tested for mutagenicity with the strains TA 100, TA 1535,TA 1537 and TA 98 of Salmonella Typhimurium.
In the toxicity test using histidine enriched agar plates and a dilution of the tester strain TA 100, which was performed with the secound experiment, toxicity was found at concentrations of 2500 µg/plate and above in the absence.
Mutagenicity: In the absence and in the presence of the metabolic activiation system Diemethylacrylsäuremethylester did not result in any relevant increases in the number of revertants in any of the bacterial strains.
Summarizing it can be stated that Dimethylacrylsäuremethylester is not mutagenic in these bacterial test systems either with or without exogenous metabolic activation at the dose level investigated.
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