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EC number: 211-470-3 | CAS number: 646-24-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-08-31 to 2012-10-24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
PHYSICO-CHEMICAL PROPERTIES
- Water solubility: 80 g /100 g - Vehicle:
- no
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchnerella subcapitata, old name: Selenastrum capricornutum
- Strain: subcapitata (CCAP 278/4)
- Age of inoculum (at test initiation): 3 days
- Any deformed or abnormal cells observed: Microscopic observation to verify a normal and healthy appearance of the inoculum culture and to observe any abnormal appearance of the algae (as may be caused by the exposure to the test substance) at the end of the test was performed. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 25 ± 2°C
- pH:
- Negative Control Group - pH at Day 0 : 6.27
Positive Control Group (Potassium dichromate) - pH at Day 0 : 5.58
NMDA, 50mg/l - pH at Day 0 : 7.01
NMDA, 100mg/l - pH at Day 0 : 7.76
NMDA, 125mg/l - pH at Day 0 : 8.45
NMDA, 150mg/l - pH at Day 0 : 9.45
NMDA, 175mg/l - pH at Day 0 : 9.89 - Nominal and measured concentrations:
- nominal concentrations: 50 mg/l, 100 mg/l, 125 mg/l, 150 mg/l and 175 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: flask
- Initial cells density: 5,000 to 50,000 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3
TEST SYSTEM VALIDATION
Three negative control replicates were performed. The biomass in the negative control cultures should increase exponentially within the 48-hour test period with a specific growth rate of at least 0.92 day-1.
The mean coefficient of variation for section-by-section specific growth rates (days 0-1 and 1-2 for 48-hour tests) in the negative control cultures must not exceed 35 %. This criterion applies to the mean value of coefficients of variation
calculated for replicate control cultures.
The coefficient of variation of average specific growth rates during the whole test period in replicate negative control cultures must not exceed 7 % in tests with Pseudokirchneriella subcapitata (Selenastrum capricornutum).
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used: 3N-BBM+V (Bold Basal Medium with 3-fold Nitrogen and Vitamins; modified) recommended by the Culture Collection Supplier for the Selenastrum capricornutum:
Stock solutions:
1 NaNO3 5.0g/200ml distilled water
2 CaCl2.2H2O 0.5g/200ml distilled water
3 MgSO4. 7H2O 1.5g/200ml distilled water
4 K2HPO4. 3H2O 1.5g/200ml distilled water
5 KH2PO4 3.5g/200ml distilled water
6 NaCl 0.5g/200ml distilled water
7 Trace element solution (Na2EDTA): add 0.375g Na2EDTA to 500 ml of distilled water first, and then add the minerals in exactly the following sequence: FeCl3.6H2O 48.5 mg, MnCl2.4H2O 20.5 mg, ZnCl2 2.5 mg, CoCl2.6H2O 1.0 mg, Na2MoO4.2H2O 2.0 mg.
8 Vitamin B1 stock solution: 0.012g Thiaminhydrochloride in 10 ml distilled water.
9 Vitamin B12 stock solution: 0.01g Cyanobalamin in 10 ml distilled water.
For 100 ml final medium:
1 NaNO3: 3.0 ml of stock
2 CaCl2.2H2O: 1.0 ml of stock
3 MgSO4. 7H2O: 1.0 ml of stock
4 K2HPO4. 3H2O: 1.0 ml of stock
5 KH2PO4: 1.0 ml of stock
6 NaCl: 1.0 ml of stock
7 Trace element solution: 0.6 ml of stock
8 Vitamin B1 stock solution: 0.1 ml of stock
9 Vitamin B12 stock solution: 0.1 ml of stock
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: by Epifluorescence Microscopy
TEST CONCENTRATIONS
- Range finding study: The concentration of 100 mg/l of the test item in algal culture media was used for the limit test.
- Test concentrations: 50 mg /l, 100 mg/l, 125 mg/l, 150 mg/l and 175 mg/l - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (K2Cr2O7)
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 93.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no - Validity criteria fulfilled:
- yes
- Remarks:
- Negative Control: specific growth rate of at least 0.92 day-1; the mean coefficient of variation for section-by-section specific growth rates of not exceeding 35 % and the coefficient of variation of average specific growth rates of not exceeding 7 %.
- Conclusions:
- Based on the above study, the EC50 of the test item nonamethylenediamine (NMDA) is 93.5mg/l.
Reference
% Inhibition of specific average growth rate related to the concentration of nonamethylenediamine.
Concentration of NMDA in algal culture media (mg/l) |
Logarithm10 of concentration |
% Inhibition of specific average growth rate |
50 |
1.70 |
7.8 |
100 |
2.00 |
33.0 |
125 |
2.10 |
80.6 |
150 |
2.18 |
100 |
Description of key information
Based on a reliable study according to OECD 201 the EC50 of the test item NMDA is 93.5mg/l.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 93.5 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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