Dihydrogen (ethyl)[4-[[4-[ethyl(3-sulphonatobenzyl)amino]phenyl](2-sulphonatophenyl)methylene]cyclohexa-2,5-dien-1-ylidene](3-sulphonatobenzyl)ammonium, aluminium salt

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

experimental data of read across substances

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on four toxicity to microorganism studies as-
WoE 2 and WoE 3.

GLP compliance:

not specified

Analytical monitoring:

not specified

Details on sampling:

WoE 2 and WoE 3:
- Concentrations: Test chemical concentration used for the study was 0.1% (1000 mg/l) and 1% (10000 mg/l), respectively.

Vehicle:

not specified

Test organisms (species):

Paramaecium caudatum

Details on inoculum:

WoE 2 and WoE 3:
- Other: Cultures of Paramecium caudatum (PC): PC was maintained at 22°C on 0.15 % dried lettuce infusion and fed with Aerobacter aerogenes.

Test type:

static

Water media type:

freshwater

Total exposure duration:

20 min

Test temperature:

WoE 2 and WoE 3: 22°C

pH:

WoE 2 and WoE 3: 7.0

Details on test conditions:

WoE 2 and WoE 3:
TEST SYSTEM
- Test vessel: Slide glass
- Biomass loading rate: 30 to 40 Paramaecium caudatum/conc.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The mean survival time and the death rate were calculated. The survivial time was defined as the time required until death was observed for each concentration. Death was assumed to have occurred when there was no movement. The death rate was defined as the precentage of deaths observed during 20 min.

Reference substance (positive control):

not specified

Key result

Duration:

20 min

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Mortality

Remarks on result:

other: WoE 2

Key result

Duration:

20 min

Dose descriptor:

other: EC

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Mortality

Remarks on result:

other: WoE 2: Death rate = 63.3%

Key result

Duration:

20 min

Dose descriptor:

other: EC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Mortality

Remarks on result:

other: WoE 3: Death rate = 36.7%

WoE 2:

Table: Effect of food dyes on the mean survival time and death rate of Paramecium caudatum.

 

Food dyes	Test chemical concentration
	1.0%		0.1%
	Mean survival time (sec)	Death rate (%)	Mean survival time (sec)	Death rate (%)
Test chemical	881	63.3	No deaths were observed for 20 mins	7.7

WoE 3:

Table:Effect of food dyes on the mean survival time and death rate of Paramecium caudatum.

 

Food dyes	Test chemical concentration
	1.0%		0.1%
	Mean survival time (sec)	Death rate (%)	Mean survival time (sec)	Death rate (%)
Test chemical	Impossible to observe microscopically because of high concentration.	Impossible to observe microscopically because of high concentration.	1037	36.7

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 20 mins NOEC value can be expected to be 1000 mg/l and EC50 value can be expected to be in the range 1000 to 10000 mg/l, respectively.

Executive summary:

Data available of the structurally and functionally similar read across chemicals has been reviewed to determine the effect of the test chemical on toxicity to microorganisms. The studies are as mentioned below:

Toxicity to microorganisms study was carried out for 20 mins. Paramaecium caudatum (Pc) was used as a test organism. Test organism was maintained at 22°C on 0.15 % dried lettuce infusion and fed with Aerobacter aerogenes. Test organisms were exposed to chemical conc. (0.1% (1000 mg/l) and 1% (10000 mg/l)) in a hollow glass slide. An equal volume of 0.04 M phosphate buffer (pH 7.0) was added. in the test vessel. After 5 to 10 Pc were added, their survival time were measured microscopically. 30 to 40 Paramaecium caudatum/conc. were tested by the same method. After an exposure period of 20 mins, the mean survival time and the death rate were calculated. The survival time was defined as the time required until death was observed for each concentration. Death was assumed to have occurred when there was no movement. The death rate was defined as the percentage of deaths observed during 20 min. On the basis of the effect of test chemical on mortality of the test organism Paramaecium caudatum, the 20 mins NOEC and EC value was determined to be 1000 and 10000 mg/l, respectively.

Another toxicity to microorganisms study was carried out for 20 mins. Paramaecium caudatum (Pc) was used as a test organism. Test organism was maintained at 22°C on 0.15 % dried lettuce infusion and fed with Aerobacter aerogenes. Study was performed using the same method as mentioned in above study. On the basis of the effect of test chemical on mortality of the test organism Paramaecium caudatum, the 20 mins EC value was determined to be 1000 mg/l.

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 20 mins NOEC value can be expected to be 1000 mg/l and EC50 value can be expected to be in the range 1000 to 10000 mg/l, respectively.

Description of key information

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 20 mins NOEC value can be expected to be 1000 mg/l and EC50 value can be expected to be in the range 1000 to 10000 mg/l, respectively.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

1 000 mg/L

Additional information

Data available of the structurally and functionally similar read across chemicals has been reviewed to determine the effect of the test chemical on toxicity to microorganisms. The studies are as mentioned below:

 

Toxicity to microorganism study was carried out for 20 mins. Paramaecium caudatum (Pc) was used as a test organism. Test organism was maintained at 22°C on 0.15 % dried lettuce infusion and fed with Aerobacter aerogenes. Test organisms were exposed to chemical conc. (0.1% (1000 mg/l) and 1% (10000 mg/l)) in a hollow glass slide. An equal volume of 0.04 M phosphate buffer (pH 7.0) was added. in the test vessel. After 5 to 10 Pc were added, their survival time were measured microscopically. 30 to 40 Paramaecium caudatum/conc. were tested by the same method. After an exposure period of 20 mins, the mean survival time and the death rate were calculated. The survival time was defined as the time required until death was observed for each concentration. Death was assumed to have occurred when there was no movement. The death rate was defined as the percentage of deaths observed during 20 min. On the basis of the effect of test chemical on mortality of the test organism Paramaecium caudatum, the 20 mins NOEC and EC value was determined to be 1000 and 10000 mg/l, respectively.

 

Another toxicity to microorganism study was carried out for 20 mins. Paramaecium caudatum (Pc) was used as a test organism. Test organism was maintained at 22°C on 0.15 % dried lettuce infusion and fed with Aerobacter aerogenes. Study was performed using the same method as mentioned in above study. On the basis of the effect of test chemical on mortality of the test organism Paramaecium caudatum, the 20 mins EC value was determined to be 1000 mg/l.

 

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 20 mins NOEC value can be expected to be 1000 mg/l and EC50 value can be expected to be in the range 1000 to 10000 mg/l, respectively.