Benzenethiol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2001

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Benzenethiol

EC Number:

203-635-3

EC Name:

Benzenethiol

Cas Number:

108-98-5

Molecular formula:

C6H6S

IUPAC Name:

benzenethiol

Details on test material:

- Name of test material : benzenthiol
- Molecular formula : C6H6S
- Molecular weight : 110.18
- Substance type: liquid (a clear, colorless)
- Analytical purity: >=98.0%

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
- Melting point: -14.8℃
- Boiling point: 168℃
- Vapour pressure:0.13kPa (18℃)
- Water solubility (under test conditions): 836mg/L
- log Pow:2.52
- pKa: 6.62

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Test solution (1.0mL~1.5mL) was obtained each test aquarium and analyzed by HPLC.
Graph of Standard curve which was obtained by standard concentration (20,60,100mg/L) led standard factor,and this factor used to obtained concentration of test solution.

Test solutions

Vehicle:

yes

Details on test solutions:

- Method:
(1)After adding test substances(30.3 mg/L) to dilution water,each concentration of test solutions prepared .
(2)The dilution water only had positive control.
(3)Each test vessels was checked in appearance.

Test organisms

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Oryzias latipes
- Length at study initiation : 2.27 cm (2.10~2.43 cm), n=10
- Weight at study initiation : 0.1842g (0.1474 ~0.2514 g), n=10
- Lot : 000402
- Feeding during test : no
- Food type: TetraMin
- Method of breeding: 10 animals/one concentration
- Light intensity : interior light ,16-hour light/8-hour dark cycle
- Amout of test solution: 3L test solution

ACCLIMATION
- Acclimation period: more than 12 days
- Acclimation conditions : same as test
- Type and amount of food: TetraMin (freeding until 24 hours before the test was started)
- Health during acclimation :0.0% mortality 7days before exposure

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

no

Test conditions

Hardness:

41.0mg (as CaCO3 ) (dilution water)

Test temperature:

24± 1℃ Temperature was cheked 24 hour intervals.

pH:

pH6.8 (dilution water) pH was cheked 24 hour intervals.

Dissolved oxygen:

Dissolved oxygen was cheked 24 hour intervals.

Salinity:

not applicable

Nominal and measured concentrations:

not applicable

Details on test conditions:

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

TEST SYSTEM
- Test vessel:3L glass beaker
- Material, size, headspace, fill volume: 3L
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): semi-static
- Renewal rate of test solution : 24 hours

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water was dechlorination with tap water in Nagoya city.
- Intervals of water quality measurement: 24 hour interval

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.009 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% (0.003~0.018 mg/L)

Details on results:

- Behavioural abnormalities: Abnormal breathing,abnormal immobilisation were observed at 0.018mg/Land,0.056mg/L, but control and less than 0.003mg/L concentrations were obseved nothing.

-Test conditions: measured pH was pH6.8~7.1 during 96 hour, measured dissolved oxgen was 5.6~9.7 mg/L and all test aquarium was no less than 60%. measured temparature was 23.4~23.9 ℃ during 96 hour.

- maximum concentration causing no mortality after 96 hours : 0.003 mg/L, minimum concentration causing 100% mortality after 96 hour : 0.056 mg/L

-test subustance concentration : the begining of test:<0.0008~0.489 mg/L ,24 hour later :<0.0008~0.284 mg/L. The nominal concentration was deviated from ±20%, the results was geometric mean of measured concentration.

Reported statistics and error estimates:

Binomial method was employed to caluculation LD50 and confidence limits(p=0.95).
Binomial method was used TOXDAT Multi-Method Program.