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EC number: 248-394-5 | CAS number: 27310-25-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted following OECD guideline, but report does not mention whether GLP was followed and sufficient data is available for interpretation of results.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- no
- Principles of method if other than guideline:
- OECD guideline 302 B followed.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 7-aminonaphthalene-1,3,5-trisulphonic acid
- EC Number:
- 248-394-5
- EC Name:
- 7-aminonaphthalene-1,3,5-trisulphonic acid
- Cas Number:
- 27310-25-4
- Molecular formula:
- C10H9NO9S3
- IUPAC Name:
- 7-aminonaphthalene-1,3,5-trisulfonic acid
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): FAT 93527/A
- Physical state: Powder
- Analytical purity: approx. 50%
- Lot/batch No.: 0031026A0
- Expiration date of the lot/batch: 10.01.2006
- Storage condition of test material: room temperature
- Solubility: approx. 120 g/L
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
None
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Mixture of polyvalent bacteria (activated sludge) collected on May 08, 2001 from the aeration tank of a domestic sewage treatment plant, PRO RHENO Basel communal.
- Preparation of inoculum for exposure: The activated sludge was washed twice with tap water one day before the test was started. Subsequently the sludge was aerated until use. A sample of the activated sludge was taken to determine the dry weight of the suspended solids.
- Concentration of sludge: 0.47 g/L suspended solids The bacteria concentration was obtained by adding 88 mL of the pre-treated sludge (11.4 g/L SS) per 2 l itres test medium.
- Initial cell/biomass concentration: 153.5 and 153.8 mg/L DOC of the test item - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 153.5 mg/L
- Based on:
- DOC
- Initial conc.:
- 153.8 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Test medium-The mineral salts for each of the following stock solutions were dissolved in 1 litre of distilled water:
Stock solution A
8.5 g of potassium acid phosphate, 21.75 g of di-potassium hydrogen phosphate, 33.4 g of di-sodium hydrogenpnospnate dihydrate and 0.5 g of ammonium chloride.
The pH value of this solution was 7.4.
Stock solution B
36.4 g of calcium chloride dihydrate
Stock solution C
22.5 g of magnesium sulphate heptahydrate
Stock solution D
0.25 g of ferric chloride hexahydrate
10 ml of solution (A) and 1 ml_ of solutions (B), (C) and (D) were added per 1 litre distilled water to form the test medium.
- Test temperature: 20 - 25 °C
- pH: 7.0 - 8.0
- pH adjusted: yes with NaOH
- Suspended solids concentration: 0.47 g/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus:Glass vessel
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: agitated and aerated
- Measuring equipment: TOC/DOC Analyzer SHIMADZU TOC 500
STATISTICAL METHODS: No Statistics applied
Results and discussion
- Preliminary study:
- None
- Test performance:
- 0% adsorption of FAT 93527/A after 3 hours
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 11
- Sampling time:
- 28 d
- Details on results:
- Adsorption:
0% adsorption of FAT 93527/A after 3 hours
Biodegradation:
11 % biodegradation of FAT 93527/A after 28 days
Total elimination:
(regarding adsorption and biodégradation)
Total elimination of FAT 93527/A after 28 days is 8 %
BOD5 / COD results
- Results with reference substance:
- Biodegradation of the reference item after 19 days is 99%
Any other information on results incl. tables
None
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- 11% biodegradation was observed after 28 days.
- Executive summary:
FAT 93527/A was tested for inherent biodegradability following OECD test guideline 302 B.
Mixture of polyvalent bacteria (activated sludge) collected on May 08, 2001 from the aeration tank of a domestic sewage treatment plant, PRO RHENO Basel communal. The activated sludge was washed twice with tap water one day before the test was started. Subsequently the sludge was aerated until use. A sample of the activated sludge was taken to determine the dry weight of the suspended solids.
The day prior to the start of the test, the stock solutions were prepared. The stock solution was diluted 1 : 1 with test water including activated sludge to get the final test concentration of 153.5 and 153.8 mg/L DOC of the test item.
A mixture of a total test volume of 2 litres each containing test item, mineral medium and activated sludge was agitated and aerated at 20 - 25 °C in two glass vessels for 28 days. A reference control running in duplicate containing activated sludge, mineral medium and the reference item, and a blank control containing activated sludge and mineral medium were run under the same test conditions. The biodegradation process was monitored by determination of the dissolved organic carbon concentrations (DOC). The ratio of eliminated DOC, corrected for the blank after each time interval, to the initial DOC value was expressed as the percentage biodegradation at each sampling time.
Prior to the DOC-determinations the particles of the activated sludge were separated from the samples by centrifugation ( 4000 rpm for 20 minutes). DOC determination was done using TOC/DOC Analyzer (SHIMADZU TOC 500).
At the end of the 28day test period 11% Biodegradation of FAT 93527/A was observed.
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