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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
abstract

Data source

Reference
Reference Type:
other: abstract
Title:
Unnamed
Year:
1993
Report date:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
4-(hydroxymethyl)-4-methyl-1-phenylpyrazolidin-3-one
EC Number:
235-920-3
EC Name:
4-(hydroxymethyl)-4-methyl-1-phenylpyrazolidin-3-one
Cas Number:
13047-13-7
Molecular formula:
C11H14N2O2
IUPAC Name:
4-(hydroxymethyl)-4-methyl-1-phenylpyrazolidin-3-one
Test material form:
solid: particulate/powder
Specific details on test material used for the study:
no batch number in this part of the reported data

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
used as such

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Pseudonomas putida (MIGULA, Stamm Berlin)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h

Test conditions

Test temperature:
21 ± 1 °C
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
16 h
Dose descriptor:
EC10
Effect conc.:
230 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
16 h
Dose descriptor:
EC50
Effect conc.:
480 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Any other information on results incl. tables

Concentration (mg/L)

Percentage inhibition (compared to initial cell count)

74

7

148

10

297

19

594

63

 

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The EC50 as derived in this study is 480 mg/L