N,N'-1,4-phenylenebis[3-oxobutyramide]

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005-08-16 to 2005-10-21

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: according to international Guidelines without relevant deviations

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-1,4-phenylenebis[3-oxobutyramide]

EC Number:

246-438-8

EC Name:

N,N'-1,4-phenylenebis[3-oxobutyramide]

Cas Number:

24731-73-5

Molecular formula:

C14H16N2O4

IUPAC Name:

3-oxo-N-[4-(3-oxobutanamido)phenyl]butanamide

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: direct application resp. dilution of stock solution
- Eluate:
- Differential loading: yes: 1 - 10 - 100 - 1000 mg/L
- Controls: yes

- Evidence of undissolved material (e.g. precipitate, surface film, etc): yes

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Remarks:

organic carbon

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
Effect concentrations exceeding solubility of substance in test medium: yes

Results with reference substance (positive control):

- Results with reference substance valid? yes

Any other information on results incl. tables

Respiration Rates and Inhibition of the Respiration

From the oxygen depletion the respiration rates were calculated in mg O2/L⋅h (Table 1).

The inhibition of the respiration rates from the test and reference item concentrations in % was calculated in comparison with the respiration rates from the control.

Table 1:Respiration Rates and Inhibition of Respiration of Test and Reference Item Concentrations

	Concentration [mg/L]	Respiration rate [mgO2/L⋅h]	Inhibition [%]
Test item	1000	24.8	-5
	100	23.2	2
	10	20.0	15
	1	24.4	-3
Control 1	–	23.2	–
Control 2	–	24.0	–
Reference item	180	6.8	71
	100	14.8	37
	58	19.6	17

 

Difference of the control respiration rates: 3 %

Promotion is signed with minus (-)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test item Diacetessig-p-phenylendiamid is not toxic at concentrations ≤ 1000 mg/L to activated sludge of a municipal sewage treatment plant.

Executive summary:

A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was performed with the test item Diacetessig-p-phenylendiamid on 2005-09-30 at DR.U.NOACK-LABORATORIEN, D-31157 Sarstedt. Test system was activated sludge of the municipal treatment plant of D-31157 Sarstedt. The test was performed under static conditions with the test item concentrations 1 - 10 - 100 - 1000 mg/L. The respiration rates of control, reference and test item concentrations were measured after a contact time of three hours, and the inhibitory effects of the test and reference concentrations were determined in comparison to the control respiration rates. No inhibition was observed at the tested concentrations. In order to check the activity of the test system and the test conditions a reference test was carried out with copper-(II)-sulphate-pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 122 mg/L.