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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 432-170-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation / corrosion
- Remarks:
- in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to OECD guideline 431 and EC method B40
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.40 (In Vitro Skin Corrosion: Transcutaneous Electrical Resistance Test (TER))
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Human Skin Model Test)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Harlan cytotest cell research GmbH
Test material
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- orange solid powder
Constituent 1
Test animals
- Details on test animals or test system and environmental conditions:
- Human skin model test.
Test system
- Amount / concentration applied:
- 25 mg test material per tissue.
- Duration of treatment / exposure:
- 3 minutes and 1 hour.
Results and discussion
In vivo
Results
- Irritation parameter:
- other: Relative absorbance
- Basis:
- mean
- Time point:
- other: 3
- Score:
- > 50
- Max. score:
- 80
- Reversibility:
- no data
- Remarks on result:
- other: In vitro study
- Irritant / corrosive response data:
- Not corrosive to skin according UN GHS / EU CLP Cat.1 / DSD
Any other information on results incl. tables
Dose group | Exposure interval | Absorbance 570 nm Tissue 1* | Absorbance 570 nm Tissue 2* | Mean absorbance of 2 tissues | Rel. Absorbance [% of negative control]** |
Negative control | 3 min | 1.345 | 1.377 | 1.361 | 100 |
Positive control | 3 min | 0.073 | 0.090 | 0.081 | 6.0 |
D-100 | 3 min | 1.074 | 0.904 | 0.989 | 72.7 |
Negative control | 1 hour | 1.152 | 1.229 | 1.190 | 100.0 |
Positive control | 1 hour | 0.021 | 0.011 | 0.016 | 1.3 |
D-100 | 1 hour | 1.005 | 0.916 | 0.960 | 80.7 |
* Mean of three replicate wells after blank correction
**relative absorbance: = 100 x (absorbance of test item)/ (absorbance of negative control)
The optical evaluation of the MTT-reducing capacity of the test item after one hour incubation with MTT-reagent did not show evidence of a blue colour and thereby was not considered to be a MTT reducer.
The test item D-100 is considered to be non-corrosive to skin:
- since the viability after 3 minutes exposure is greater than 50 % and
- the viability after 1 hour exposure is greater than 15%.
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Remarks:
- Migrated information
- Conclusions:
- Not corrosive to skin according UN GHS / EU CLP Cat.1 / DSD
- Executive summary:
This in vitro study was performed to assess the corrosive potential of D-100 by means of the Human Skin Model Test. Independent duplicate tissues of the human skin model EST-1000™ were exposed to the test item, the negative control or the positive control for 3 minutes and 1 hour, respectively. About 25 mg of the solid test material were applied to the tissues and wetted with 50 μL deionised water. A volume of 50 μL of either the negative control (deionised water) or the positive control (8.0 N KOH) was applied to each tissue. After exposure to the negative control the absorbance values exceeded the required acceptability criterion of mean OD570 ≥ 0.8 for both treatment intervals thereby confirming the acceptable quality of the tissues. Exposure to the positive control induced a decrease in the relative absorbance as compared to the negative control, both for the 3 minutes exposure period and for the 1 hour exposure period thus confirming the validity of the test system. After exposure to the test item D-100 the relative absorbance values decreased to 72.7% after 3 minutes. After the 1 hour exposure relative absorbance values were reduced to
80.7%. Nevertheless, both values are well above the threshold of 50% for the 3 minutes exposure and above 15% for the 1 hour exposure. Therefore, the test item was considered to be not corrosive. In conclusion, it can be stated that in this study and under the reported experimental conditions, the test item D-100 was non corrosive to skin according to UN GHS / EU CLP Cat.1 / DSD (67/548/EEC).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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