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EC number: 268-069-1 | CAS number: 68002-54-0
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
Under the conditions of this study, the EL50 for growth was determined to be 31 mg/L loading rate WAF, with the NOELR and LOELR 0.32 and 1.0 mg/L, respectively.
As a result of the EL50 value, the test material requires classification for being hazardous to the aqueous environment and as such is categorised as Chronic Category 3 in accordance with EU criteria.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 31 mg/L
- EC10 or NOEC for freshwater algae:
- 0.32 mg/L
Additional information
The key study was conducted in line with GLP and the standardised guidelines OECD 201and EU Method C.3 and assessed the effect of the test material on the growth of the green alga Pseudokirchneriella subcapitata. It was assigned a reliability score of 1 in accordance with the criteria detailed by Klimisch (1997).
Due to the low aqueous solubility and complex nature of the test material, it was prepared as a Water Accommodated Fraction (WAF).
Following preliminary range-finding tests, the algal cells were exposed to WAFs of the test material over a range of nominal loading rates of 0.10, 0.32, 1.0, 3.2 and 10 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ± 1 °C.
Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group.
Under the conditions of this study, the EL50 for growth was determined to be 31 mg/L loading rate WAF, with the NOELR and LOELR 0.32 and 1.0 mg/L, respectively.
Analysis of the test preparations at 0 hours showed measured test concentrations to range from less than the limit of quantitation of the analytical method employed (which was determined to be 0.25 mg/L) at 0.10 mg/L loading rate WAF through to 11 mg/L at 10 mg/L loading rate WAF. A significant decline in measured test concentration was observed at 72 hours to less than the LOQ for all loading rates.
Given that the toxicity cannot be attributed to a single component or a mixture of components but to the test item as a whole the results were based on nominal loading rates only.
As a result of the EL50 value, the test material requires classification for being hazardous to the aqueous environment and as such is categorised as Chronic Category 3 in accordance with EU criteria.
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